Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri
Abstract Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-05-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.706 |
| _version_ | 1818190047749341184 |
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| author | Malgorzata M. Kopacz André S. G. Lorenzoni Carlos R. Polaquini Luis O. Regasini Dirk‐Jan Scheffers |
| author_facet | Malgorzata M. Kopacz André S. G. Lorenzoni Carlos R. Polaquini Luis O. Regasini Dirk‐Jan Scheffers |
| author_sort | Malgorzata M. Kopacz |
| collection | DOAJ |
| description | Abstract Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds. |
| first_indexed | 2024-12-11T23:52:30Z |
| format | Article |
| id | doaj.art-9ac1b1ecf9f44df982dcccd6a149c18b |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-11T23:52:30Z |
| publishDate | 2019-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-9ac1b1ecf9f44df982dcccd6a149c18b2022-12-22T00:45:27ZengWileyMicrobiologyOpen2045-88272019-05-0185n/an/a10.1002/mbo3.706Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citriMalgorzata M. Kopacz0André S. G. Lorenzoni1Carlos R. Polaquini2Luis O. Regasini3Dirk‐Jan Scheffers4Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen The NetherlandsDepartment of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen The NetherlandsLaboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences, Humanities and Exact Sciences São Paulo State University (UNESP) São José do Rio Preto SP BrazilLaboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences, Humanities and Exact Sciences São Paulo State University (UNESP) São José do Rio Preto SP BrazilDepartment of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen The NetherlandsAbstract Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.https://doi.org/10.1002/mbo3.706antibacterialscell divisionFtsZgallatesgallic acidGTPase |
| spellingShingle | Malgorzata M. Kopacz André S. G. Lorenzoni Carlos R. Polaquini Luis O. Regasini Dirk‐Jan Scheffers Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri MicrobiologyOpen antibacterials cell division FtsZ gallates gallic acid GTPase |
| title | Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
| title_full | Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
| title_fullStr | Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
| title_full_unstemmed | Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
| title_short | Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
| title_sort | purification and characterization of ftsz from the citrus canker pathogen xanthomonas citri subsp citri |
| topic | antibacterials cell division FtsZ gallates gallic acid GTPase |
| url | https://doi.org/10.1002/mbo3.706 |
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