Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR

Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR

Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;q11). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia....

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Bibliographic Details
Main Authors: Aya Bonilla, Carlos Alberto, Domingo Torres, José, Muskus, Carlos Enrique, Ramírez Gaviria, Gloria, Cuervo Sierra, Jorge, Sierra Sánchez, Margarita, Cuéllar-Ambrosi, Francisco, Botero Garcés, Jorge Humberto, Gloria Artigas A., Carmen, Muñetón, Carlos Mario, Vásquez Palacio, Gonzalo
Format: Article
Language:Spanish
Published: Universidad de Antioquia 2014-10-01
Series:Iatreia
Subjects:
Leucemia Mieloide Crónica
PCR Anidada
Proteínas de Fusión bcr-abl
Leukemia
Myelogenous
Chronic
Nested PCR
Fusion Proteins
bcr-abl
Leucemia Mielóide Crônica
PCR Aninhada
Proteínas de Fusão bcr-abl
Online Access:http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/15539/17392
Description
Summary:Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;q11). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia. Objective: To determine the frequency of BCR-ABL gene fusions encoding the p210BCR-ABL y p190 BCR-ABL transcripts in Colombian patients diagnosed with CML in different stages of the disease and/or its treatment. Materials and methods: Cross sectional, descriptive study of thirty one CML patients (aged 15-78). Analysis was carried out through qualitative nested PCR for the isoforms P210 BCR-ABL (b3a2 e b2a2) and P190 BCR-ABL (e1a2), and based on peripheral blood samples. Results: In 29 of the 31 patients (93.6%) transcript p210BCR-ABL was detected; b2a2 and b3a2 gene fusions and the coexpression b3a2 y b2a2 were identified in 55.2% (16/29), 34.5% (10/29) and 10.3% (3/29) of the cases, respectively. Conclusion: b2a2 gene fusion was the most frequent in this CML population.
ISSN:0121-0793
2011-7965

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