Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR
Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;q11). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad de Antioquia
2014-10-01
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| Series: | Iatreia |
| Subjects: | |
| Online Access: | http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/15539/17392 |
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| author | Aya Bonilla, Carlos Alberto Domingo Torres, José Muskus, Carlos Enrique Ramírez Gaviria, Gloria Cuervo Sierra, Jorge Sierra Sánchez, Margarita Cuéllar-Ambrosi, Francisco Botero Garcés, Jorge Humberto Gloria Artigas A., Carmen Muñetón, Carlos Mario Vásquez Palacio, Gonzalo |
| author_facet | Aya Bonilla, Carlos Alberto Domingo Torres, José Muskus, Carlos Enrique Ramírez Gaviria, Gloria Cuervo Sierra, Jorge Sierra Sánchez, Margarita Cuéllar-Ambrosi, Francisco Botero Garcés, Jorge Humberto Gloria Artigas A., Carmen Muñetón, Carlos Mario Vásquez Palacio, Gonzalo |
| author_sort | Aya Bonilla, Carlos Alberto |
| collection | DOAJ |
| description | Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;q11). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia.
Objective: To determine the frequency of BCR-ABL gene fusions encoding the p210BCR-ABL y p190 BCR-ABL transcripts in Colombian patients diagnosed with CML in different stages of the disease and/or its treatment.
Materials and methods: Cross sectional, descriptive study of thirty one CML patients (aged 15-78). Analysis was carried out through qualitative nested PCR for the isoforms P210 BCR-ABL (b3a2 e b2a2) and P190 BCR-ABL (e1a2), and based on peripheral blood samples.
Results: In 29 of the 31 patients (93.6%) transcript p210BCR-ABL was detected; b2a2 and b3a2 gene fusions and the coexpression b3a2 y b2a2 were identified in 55.2% (16/29), 34.5% (10/29) and 10.3% (3/29) of the cases, respectively.
Conclusion: b2a2 gene fusion was the most frequent in this CML population. |
| first_indexed | 2024-12-11T03:40:44Z |
| format | Article |
| id | doaj.art-9ac32f8e3d3b4e64aa5110ee24dd7c93 |
| institution | Directory Open Access Journal |
| issn | 0121-0793 2011-7965 |
| language | Spanish |
| last_indexed | 2024-12-11T03:40:44Z |
| publishDate | 2014-10-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Iatreia |
| spelling | doaj.art-9ac32f8e3d3b4e64aa5110ee24dd7c932022-12-22T01:22:09ZspaUniversidad de AntioquiaIatreia0121-07932011-79652014-10-01274398409Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCRAya Bonilla, Carlos Alberto 0Domingo Torres, José 1 Muskus, Carlos Enrique2Ramírez Gaviria, Gloria 3Cuervo Sierra, Jorge 4Sierra Sánchez, Margarita 5Cuéllar-Ambrosi, Francisco 6Botero Garcés, Jorge Humberto7Gloria Artigas A., Carmen 8Muñetón, Carlos Mario 9Vásquez Palacio, Gonzalo10Universidad de AntioquiaUniversidad de AntioquiaUniversidad de AntioquiaUniversidad de AntioquiaHospital Universitario UANL de MonterreyHospital Universitario San Vicente FundaciónUniversidad de AntioquiaUniversidad de AntioquiaUniversidad San SebastiánUniversidad de AntioquiaUniversidad de AntioquiaIntroduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;q11). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia. Objective: To determine the frequency of BCR-ABL gene fusions encoding the p210BCR-ABL y p190 BCR-ABL transcripts in Colombian patients diagnosed with CML in different stages of the disease and/or its treatment. Materials and methods: Cross sectional, descriptive study of thirty one CML patients (aged 15-78). Analysis was carried out through qualitative nested PCR for the isoforms P210 BCR-ABL (b3a2 e b2a2) and P190 BCR-ABL (e1a2), and based on peripheral blood samples. Results: In 29 of the 31 patients (93.6%) transcript p210BCR-ABL was detected; b2a2 and b3a2 gene fusions and the coexpression b3a2 y b2a2 were identified in 55.2% (16/29), 34.5% (10/29) and 10.3% (3/29) of the cases, respectively. Conclusion: b2a2 gene fusion was the most frequent in this CML population.http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/15539/17392Leucemia Mieloide CrónicaPCR AnidadaProteínas de Fusión bcr-ablLeukemiaMyelogenousChronicNested PCRFusion Proteinsbcr-ablLeucemia Mielóide CrônicaPCR AninhadaProteínas de Fusão bcr-abl |
| spellingShingle | Aya Bonilla, Carlos Alberto Domingo Torres, José Muskus, Carlos Enrique Ramírez Gaviria, Gloria Cuervo Sierra, Jorge Sierra Sánchez, Margarita Cuéllar-Ambrosi, Francisco Botero Garcés, Jorge Humberto Gloria Artigas A., Carmen Muñetón, Carlos Mario Vásquez Palacio, Gonzalo Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR Iatreia Leucemia Mieloide Crónica PCR Anidada Proteínas de Fusión bcr-abl Leukemia Myelogenous Chronic Nested PCR Fusion Proteins bcr-abl Leucemia Mielóide Crônica PCR Aninhada Proteínas de Fusão bcr-abl |
| title | Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR |
| title_full | Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR |
| title_fullStr | Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR |
| title_full_unstemmed | Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR |
| title_short | Detection of p190BCR-ABL AND p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR |
| title_sort | detection of p190bcr abl and p210bcr abl fusion transcripts in patients with chronic myeloid leukemia cml using qualitative rt pcr |
| topic | Leucemia Mieloide Crónica PCR Anidada Proteínas de Fusión bcr-abl Leukemia Myelogenous Chronic Nested PCR Fusion Proteins bcr-abl Leucemia Mielóide Crônica PCR Aninhada Proteínas de Fusão bcr-abl |
| url | http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/15539/17392 |
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