Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway
Abstract Background Wu Mei Pills (WMP) is a traditional Chinese medication that exhibits considerable anti-inflammatory effects. While WMP has been documented for its efficacy in treating RA, its mechanism of action on the condition remains unestablished. Methods The chemical composition of WMP was...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-04551-z |
| _version_ | 1797355663459876864 |
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| author | Yuheng Fu Chunyu Gao Xialin Sun Yan Zhao Haibo Zhang |
| author_facet | Yuheng Fu Chunyu Gao Xialin Sun Yan Zhao Haibo Zhang |
| author_sort | Yuheng Fu |
| collection | DOAJ |
| description | Abstract Background Wu Mei Pills (WMP) is a traditional Chinese medication that exhibits considerable anti-inflammatory effects. While WMP has been documented for its efficacy in treating RA, its mechanism of action on the condition remains unestablished. Methods The chemical composition of WMP was analyzed through UPLC-MS. Next, the enzyme-linked immunosorbent assay, cell scratch, Transwell, and Western blotting techniques were used to investigate its intrinsic mechanism. Lastly, the effect of WMP in inhibiting RA was explored by applying it to CIA rats. Result UPLC-MS analysis detected 181 compounds in WMP. RA-FLS migration and invasion mechanisms were significantly hindered by serum containing WMP (2%, 8%). Moreover, WMP (0.5 g/kg, 2 g/kg) restricted arthritis and immune organ indices in CIA rats with type II collagen-induced rheumatoid arthritis by blocking TLR4-NF-κB inflammatory pathway activation. Conclusions WMP is valuable in mitigating the course of RA through inhibiting the classical TLR4-NF-κB inflammatory pathway and reducing the secretion of inflammatory factors in the serum of RA-FLS and CIA rats. Moreover, it regulates the dynamic balance of MMP-2/TIMP-2, MMP-9/TIMP-1, modulates the mechanism of RA-FLS invasion, and safeguards articular cartilage tissues in RA. |
| first_indexed | 2024-03-08T14:14:23Z |
| format | Article |
| id | doaj.art-9ac60a5c244c405b8b2b96e18170a791 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-08T14:14:23Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-9ac60a5c244c405b8b2b96e18170a7912024-01-14T12:30:17ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-01-0119111210.1186/s13018-024-04551-zStudy on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathwayYuheng Fu0Chunyu Gao1Xialin Sun2Yan Zhao3Haibo Zhang4Tianjin University of Traditional Chinese MedicineCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityCollege of Pharmacy, Jilin Medical UniversityCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityChangchun University of Chinese MedicineAbstract Background Wu Mei Pills (WMP) is a traditional Chinese medication that exhibits considerable anti-inflammatory effects. While WMP has been documented for its efficacy in treating RA, its mechanism of action on the condition remains unestablished. Methods The chemical composition of WMP was analyzed through UPLC-MS. Next, the enzyme-linked immunosorbent assay, cell scratch, Transwell, and Western blotting techniques were used to investigate its intrinsic mechanism. Lastly, the effect of WMP in inhibiting RA was explored by applying it to CIA rats. Result UPLC-MS analysis detected 181 compounds in WMP. RA-FLS migration and invasion mechanisms were significantly hindered by serum containing WMP (2%, 8%). Moreover, WMP (0.5 g/kg, 2 g/kg) restricted arthritis and immune organ indices in CIA rats with type II collagen-induced rheumatoid arthritis by blocking TLR4-NF-κB inflammatory pathway activation. Conclusions WMP is valuable in mitigating the course of RA through inhibiting the classical TLR4-NF-κB inflammatory pathway and reducing the secretion of inflammatory factors in the serum of RA-FLS and CIA rats. Moreover, it regulates the dynamic balance of MMP-2/TIMP-2, MMP-9/TIMP-1, modulates the mechanism of RA-FLS invasion, and safeguards articular cartilage tissues in RA.https://doi.org/10.1186/s13018-024-04551-zWu Mei PillRheumatoid arthritisFibroblastic synovial cellsInvasion and migrationTLR4-NF-κB pathway |
| spellingShingle | Yuheng Fu Chunyu Gao Xialin Sun Yan Zhao Haibo Zhang Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway Journal of Orthopaedic Surgery and Research Wu Mei Pill Rheumatoid arthritis Fibroblastic synovial cells Invasion and migration TLR4-NF-κB pathway |
| title | Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway |
| title_full | Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway |
| title_fullStr | Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway |
| title_full_unstemmed | Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway |
| title_short | Study on the mechanism of action of Wu Mei Pill in inhibiting rheumatoid arthritis through TLR4-NF-κB pathway |
| title_sort | study on the mechanism of action of wu mei pill in inhibiting rheumatoid arthritis through tlr4 nf κb pathway |
| topic | Wu Mei Pill Rheumatoid arthritis Fibroblastic synovial cells Invasion and migration TLR4-NF-κB pathway |
| url | https://doi.org/10.1186/s13018-024-04551-z |
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