GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells
Maryam Hafizi,1 Somayeh Kalanaky,1 Saideh Fakharzadeh,1 Ehsan Janzamin,2 Tarlan Arjmandi,1 Amir Atashi,3,* Mohammad Hassan Nazaran1,* 1Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran; 2Flowcyt Science-Based Company, Tehran, Iran; 3Stem Cell and Tissue Engineering Re...
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2020-08-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/gfc7-as-a-smart-growth-nanofactor-for-ex-vivo-expansion-and-cryoprotec-peer-reviewed-article-IJN |
| _version_ | 1818733905227808768 |
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| author | Hafizi M Kalanaky S Fakharzadeh S Janzamin E Arjmandi T Atashi A Nazaran MH |
| author_facet | Hafizi M Kalanaky S Fakharzadeh S Janzamin E Arjmandi T Atashi A Nazaran MH |
| author_sort | Hafizi M |
| collection | DOAJ |
| description | Maryam Hafizi,1 Somayeh Kalanaky,1 Saideh Fakharzadeh,1 Ehsan Janzamin,2 Tarlan Arjmandi,1 Amir Atashi,3,* Mohammad Hassan Nazaran1,* 1Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran; 2Flowcyt Science-Based Company, Tehran, Iran; 3Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran*These authors contributed equally to this workCorrespondence: Amir Atashi; Mohammad Hassan Nazaran Tel/Fax +98 23 3239 45000; +98 21 8899 2123Email atashia@shmu.ac.ir; mnazaran@nanochelatingtechnology.comBackground: Nowadays, smart synthesized nanostructures have attracted wide attention in the field of stem cell nanotechnology due to their effect on different properties of stem cells.Methods: GFc7 growth nanofactor was synthesized based on nanochelating technology as an iron-containing copper chelator nanocomplex. The effect of this nanocomplex on the expansion and differentiation of hematopoietic stem cells (HSCs) as well as its performance as a cryoprotectant was evaluated in the present study.Results: The results showed that the absolute count of CD34+ and CD34+CD38− cells on days 4, 7, 10 and 13; the percentage of lactate dehydrogenase enzyme on the same days and CD34+CXCR4 population on day 10 were significantly increased when they were treated with GFc7 growth nanofactor in a fetal bovine serum (FBS)-free medium. This medium also led to delayed differentiation in HSCs. One noticeable result was that CD34+CD38− cells cultured in an FBS medium were immediately differentiated into CD34+CD38+ cells, while CD34+CD38− cells treated with GFc7 growth nanofactor in FBS medium did not show such an immediate significant differentiation. De-freezing GFc7-treated CD34+ cells, which were already frozen according to cord blood bank protocols, showed a higher percentage of cell viability and a larger number of colonies according to colony-forming cell assay as compared to control.Conclusion: It can be claimed that treating HSCs with GFc7 growth nanofactor leads to quality and quantity improvement of HSCs, both in terms of expansion in vitro and freezing and de-freezing processes.Keywords: expansion, GFc7, human hematopoietic stem cells, nanocomplex, nanochelating technology |
| first_indexed | 2024-12-17T23:56:53Z |
| format | Article |
| id | doaj.art-9ac6a82000e14c3f860f805c4274f650 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T23:56:53Z |
| publishDate | 2020-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-9ac6a82000e14c3f860f805c4274f6502022-12-21T21:28:03ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-08-01Volume 156263627756469GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem CellsHafizi MKalanaky SFakharzadeh SJanzamin EArjmandi TAtashi ANazaran MHMaryam Hafizi,1 Somayeh Kalanaky,1 Saideh Fakharzadeh,1 Ehsan Janzamin,2 Tarlan Arjmandi,1 Amir Atashi,3,* Mohammad Hassan Nazaran1,* 1Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran; 2Flowcyt Science-Based Company, Tehran, Iran; 3Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran*These authors contributed equally to this workCorrespondence: Amir Atashi; Mohammad Hassan Nazaran Tel/Fax +98 23 3239 45000; +98 21 8899 2123Email atashia@shmu.ac.ir; mnazaran@nanochelatingtechnology.comBackground: Nowadays, smart synthesized nanostructures have attracted wide attention in the field of stem cell nanotechnology due to their effect on different properties of stem cells.Methods: GFc7 growth nanofactor was synthesized based on nanochelating technology as an iron-containing copper chelator nanocomplex. The effect of this nanocomplex on the expansion and differentiation of hematopoietic stem cells (HSCs) as well as its performance as a cryoprotectant was evaluated in the present study.Results: The results showed that the absolute count of CD34+ and CD34+CD38− cells on days 4, 7, 10 and 13; the percentage of lactate dehydrogenase enzyme on the same days and CD34+CXCR4 population on day 10 were significantly increased when they were treated with GFc7 growth nanofactor in a fetal bovine serum (FBS)-free medium. This medium also led to delayed differentiation in HSCs. One noticeable result was that CD34+CD38− cells cultured in an FBS medium were immediately differentiated into CD34+CD38+ cells, while CD34+CD38− cells treated with GFc7 growth nanofactor in FBS medium did not show such an immediate significant differentiation. De-freezing GFc7-treated CD34+ cells, which were already frozen according to cord blood bank protocols, showed a higher percentage of cell viability and a larger number of colonies according to colony-forming cell assay as compared to control.Conclusion: It can be claimed that treating HSCs with GFc7 growth nanofactor leads to quality and quantity improvement of HSCs, both in terms of expansion in vitro and freezing and de-freezing processes.Keywords: expansion, GFc7, human hematopoietic stem cells, nanocomplex, nanochelating technologyhttps://www.dovepress.com/gfc7-as-a-smart-growth-nanofactor-for-ex-vivo-expansion-and-cryoprotec-peer-reviewed-article-IJNexpansiongfc7human hematopoietic stem cellsnanocomplexnanochelating technology. |
| spellingShingle | Hafizi M Kalanaky S Fakharzadeh S Janzamin E Arjmandi T Atashi A Nazaran MH GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells International Journal of Nanomedicine expansion gfc7 human hematopoietic stem cells nanocomplex nanochelating technology. |
| title | GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells |
| title_full | GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells |
| title_fullStr | GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells |
| title_full_unstemmed | GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells |
| title_short | GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells |
| title_sort | gfc7 as a smart growth nanofactor for ex vivo expansion and cryoprotection of humans rsquo hematopoietic stem cells |
| topic | expansion gfc7 human hematopoietic stem cells nanocomplex nanochelating technology. |
| url | https://www.dovepress.com/gfc7-as-a-smart-growth-nanofactor-for-ex-vivo-expansion-and-cryoprotec-peer-reviewed-article-IJN |
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