Constitutive and evoked release of ATP in adult mouse olfactory epithelium
In adult olfactory epithelium (OE), ATP plays a role in constant cell turnover and post-injury neuroregeneration. We previously demonstrated that constitutive and ATP-evoked ATP release are present in neonatal mouse OE and underlie continuous cell turn-over and post-injury neuroregeneration, and tha...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-01-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0811 |
| _version_ | 1797349315090317312 |
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| author | Hayoz Sébastien Jia Cuihong Hegg Colleen Cosgrove |
| author_facet | Hayoz Sébastien Jia Cuihong Hegg Colleen Cosgrove |
| author_sort | Hayoz Sébastien |
| collection | DOAJ |
| description | In adult olfactory epithelium (OE), ATP plays a role in constant cell turnover and post-injury neuroregeneration. We previously demonstrated that constitutive and ATP-evoked ATP release are present in neonatal mouse OE and underlie continuous cell turn-over and post-injury neuroregeneration, and that activation of purinergic P2X7 receptors is involved in the evoked release. We hypothesized that both releases are present in adult mouse OE. To study the putative contribution of olfactory sensory neurons to ATP release, we used olfactory sensory neuronal-like OP6 cells derived from the embryonic olfactory placode cells. Calcium imaging showed that OP6 cells and primary adult OE cell cultures express functional purinergic receptors. We monitored ATP release from OP6 cells and whole adult OE turbinates using HEK cells as biosensors and luciferin–luciferase assays. Constitutive ATP release occurs in OP6 cells and whole adult mouse OE turbinates, and P2X7 receptors mediated evoked ATP release occurs only in turbinates. The mechanisms of ATP release described in the present study might underlie the constant cell turn-over and post-injury neuroregeneration present in adult OE and thus, further studies of these mechanisms are warranted as it will improve our knowledge of OE tissue homeostasis and post-injury regeneration. |
| first_indexed | 2024-03-08T12:28:33Z |
| format | Article |
| id | doaj.art-9ac89ef7c81444828854e528e522fe69 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-08T12:28:33Z |
| publishDate | 2024-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-9ac89ef7c81444828854e528e522fe692024-01-22T07:04:05ZengDe GruyterOpen Life Sciences2391-54122024-01-011911154010.1515/biol-2022-0811Constitutive and evoked release of ATP in adult mouse olfactory epitheliumHayoz Sébastien0Jia Cuihong1Hegg Colleen Cosgrove2Department of Physiology, University of Arizona, Tucson, Arizona 85724, USADepartment of Biomedical Sciences, East Tennessee State University, Johnson City, Tennessee 37614, USADepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USAIn adult olfactory epithelium (OE), ATP plays a role in constant cell turnover and post-injury neuroregeneration. We previously demonstrated that constitutive and ATP-evoked ATP release are present in neonatal mouse OE and underlie continuous cell turn-over and post-injury neuroregeneration, and that activation of purinergic P2X7 receptors is involved in the evoked release. We hypothesized that both releases are present in adult mouse OE. To study the putative contribution of olfactory sensory neurons to ATP release, we used olfactory sensory neuronal-like OP6 cells derived from the embryonic olfactory placode cells. Calcium imaging showed that OP6 cells and primary adult OE cell cultures express functional purinergic receptors. We monitored ATP release from OP6 cells and whole adult OE turbinates using HEK cells as biosensors and luciferin–luciferase assays. Constitutive ATP release occurs in OP6 cells and whole adult mouse OE turbinates, and P2X7 receptors mediated evoked ATP release occurs only in turbinates. The mechanisms of ATP release described in the present study might underlie the constant cell turn-over and post-injury neuroregeneration present in adult OE and thus, further studies of these mechanisms are warranted as it will improve our knowledge of OE tissue homeostasis and post-injury regeneration.https://doi.org/10.1515/biol-2022-0811primary cell culturesop6 cellsconfocal imagingimmunohistochemistryluciferin–luciferase assay |
| spellingShingle | Hayoz Sébastien Jia Cuihong Hegg Colleen Cosgrove Constitutive and evoked release of ATP in adult mouse olfactory epithelium Open Life Sciences primary cell cultures op6 cells confocal imaging immunohistochemistry luciferin–luciferase assay |
| title | Constitutive and evoked release of ATP in adult mouse olfactory epithelium |
| title_full | Constitutive and evoked release of ATP in adult mouse olfactory epithelium |
| title_fullStr | Constitutive and evoked release of ATP in adult mouse olfactory epithelium |
| title_full_unstemmed | Constitutive and evoked release of ATP in adult mouse olfactory epithelium |
| title_short | Constitutive and evoked release of ATP in adult mouse olfactory epithelium |
| title_sort | constitutive and evoked release of atp in adult mouse olfactory epithelium |
| topic | primary cell cultures op6 cells confocal imaging immunohistochemistry luciferin–luciferase assay |
| url | https://doi.org/10.1515/biol-2022-0811 |
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