Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat
Abstract Lateral fluid percussion injury (LFPI) in rats is used to model post‐traumatic epilepsy (PTE), with spontaneous seizures occurring in up to ½ of the subjects. Using the kindling paradigm, we examined whether animals without detectable seizures had an altered seizure susceptibility. Male Spr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | Epilepsia Open |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/epi4.12472 |
| _version_ | 1818454976966426624 |
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| author | Jesús‐Servando Medel‐Matus Don Shin Raman Sankar Andrey Mazarati |
| author_facet | Jesús‐Servando Medel‐Matus Don Shin Raman Sankar Andrey Mazarati |
| author_sort | Jesús‐Servando Medel‐Matus |
| collection | DOAJ |
| description | Abstract Lateral fluid percussion injury (LFPI) in rats is used to model post‐traumatic epilepsy (PTE), with spontaneous seizures occurring in up to ½ of the subjects. Using the kindling paradigm, we examined whether animals without detectable seizures had an altered seizure susceptibility. Male Sprague Dawley rats were subjected to LFPI. Seven‐nine months later, spontaneous seizures were monitored for two weeks. Afterward, the animals underwent kindling of basolateral amygdala. For kindling outcomes, the animals were categorized based on the 95% confidence intervals of mean number trials to kindling (ie 3 consecutive stage 4‐5 seizures). Spontaneous seizures were detected in 7 out of 24 rats. There was no correlation between the severity of LFPI and either baseline afterdischarge properties, or kindling rates. Six LFPI rats kindled at a rate comparable to those in sham‐LFPI (n = 10) and in naïve (n = 7) subjects. Ten LFPI rats kindled faster and 8—slower than controls. None of slow‐kindling rats had spontaneous seizures during the prekindling monitoring. During the same period, six fast‐kindling and three normal‐kindling rats had been seizure‐free. Thus, kindling reveals a diversity to seizure susceptibility after LFPI beyond an overt seizure symptomatology, ranging from the increased susceptibility to the increased resistance. |
| first_indexed | 2024-12-14T22:03:27Z |
| format | Article |
| id | doaj.art-9aca0fa69bc545009eb213bf182ca633 |
| institution | Directory Open Access Journal |
| issn | 2470-9239 |
| language | English |
| last_indexed | 2024-12-14T22:03:27Z |
| publishDate | 2021-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Epilepsia Open |
| spelling | doaj.art-9aca0fa69bc545009eb213bf182ca6332022-12-21T22:45:56ZengWileyEpilepsia Open2470-92392021-06-016241341810.1002/epi4.12472Diversity of kindling of limbic seizures after lateral fluid percussion injury in the ratJesús‐Servando Medel‐Matus0Don Shin1Raman Sankar2Andrey Mazarati3Department of Pediatrics David Geffen School of Medicine at UCLA Los Angeles CA USADepartment of Pediatrics David Geffen School of Medicine at UCLA Los Angeles CA USADepartment of Pediatrics David Geffen School of Medicine at UCLA Los Angeles CA USADepartment of Pediatrics David Geffen School of Medicine at UCLA Los Angeles CA USAAbstract Lateral fluid percussion injury (LFPI) in rats is used to model post‐traumatic epilepsy (PTE), with spontaneous seizures occurring in up to ½ of the subjects. Using the kindling paradigm, we examined whether animals without detectable seizures had an altered seizure susceptibility. Male Sprague Dawley rats were subjected to LFPI. Seven‐nine months later, spontaneous seizures were monitored for two weeks. Afterward, the animals underwent kindling of basolateral amygdala. For kindling outcomes, the animals were categorized based on the 95% confidence intervals of mean number trials to kindling (ie 3 consecutive stage 4‐5 seizures). Spontaneous seizures were detected in 7 out of 24 rats. There was no correlation between the severity of LFPI and either baseline afterdischarge properties, or kindling rates. Six LFPI rats kindled at a rate comparable to those in sham‐LFPI (n = 10) and in naïve (n = 7) subjects. Ten LFPI rats kindled faster and 8—slower than controls. None of slow‐kindling rats had spontaneous seizures during the prekindling monitoring. During the same period, six fast‐kindling and three normal‐kindling rats had been seizure‐free. Thus, kindling reveals a diversity to seizure susceptibility after LFPI beyond an overt seizure symptomatology, ranging from the increased susceptibility to the increased resistance.https://doi.org/10.1002/epi4.12472epileptogenesispost‐traumatic epilepsytraumatic brain injury |
| spellingShingle | Jesús‐Servando Medel‐Matus Don Shin Raman Sankar Andrey Mazarati Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat Epilepsia Open epileptogenesis post‐traumatic epilepsy traumatic brain injury |
| title | Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| title_full | Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| title_fullStr | Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| title_full_unstemmed | Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| title_short | Diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| title_sort | diversity of kindling of limbic seizures after lateral fluid percussion injury in the rat |
| topic | epileptogenesis post‐traumatic epilepsy traumatic brain injury |
| url | https://doi.org/10.1002/epi4.12472 |
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