Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays
Cytotoxic effects of the market leading broad-spectrum, synthetic herbicide product Roundup Classic, its active ingredient glyphosate (in a form of its isopropylamine (IPA) salt) and its formulating surfactant polyethoxylated tallowamine (POE-15) were determined on two murine cell lines, a neuroecto...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221475002200097X |
| _version_ | 1811295713324171264 |
|---|---|
| author | Marianna Oláh Enikő Farkas Inna Székács Robert Horvath András Székács |
| author_facet | Marianna Oláh Enikő Farkas Inna Székács Robert Horvath András Székács |
| author_sort | Marianna Oláh |
| collection | DOAJ |
| description | Cytotoxic effects of the market leading broad-spectrum, synthetic herbicide product Roundup Classic, its active ingredient glyphosate (in a form of its isopropylamine (IPA) salt) and its formulating surfactant polyethoxylated tallowamine (POE-15) were determined on two murine cell lines, a neuroectodermal stem cell-like (NE-4C) and a high alkaline phosphatase activity osteoblastic cell line (MC3T3-E1). Cytotoxicity, genotoxicity, effects on cell viability and cell cycles were examined in five flow cytometry tests, the two former of which were compared by the enzymatic-assay and the alkaline single cell gel electrophoresis (Comet) assay. All of the tests indicated the NE-4C cells being more sensitive, than the MC3T3-E1 cell line to the treatments with the target compounds. Higher sensitivity differences were detected in the viability test by flow cytometry (7–9-fold), than by the MTT assay (1.5–3-fold); in the genotoxicity test by the Comet assay (3.5–403-fold), than by the DNA-damage test (9.3–158-fold); and in the apoptosis test by the Annexin V dead cell kit (1.1–12.7-fold), than by the Caspase 3/7 kit (1–6.5-fold). Cell cycle assays indicated high count of cells (~70%) in the G0/G1 phase for MC3T3-E1 cells, than in NE-4C cell (~40%) after 24 h. The order of the inhibitory potency of the target substances has unequivocally been POE-15 > Roundup Classic > > glyphosate IPA salt. |
| first_indexed | 2024-04-13T05:37:35Z |
| format | Article |
| id | doaj.art-9acb1a350c5b4a6aa49a3871f9d97599 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-13T05:37:35Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-9acb1a350c5b4a6aa49a3871f9d975992022-12-22T03:00:14ZengElsevierToxicology Reports2214-75002022-01-019914926Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assaysMarianna Oláh0Enikő Farkas1Inna Székács2Robert Horvath3András Székács4Agro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman Ottó u. 15, H-1022 Budapest, HungaryNanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Centre for Energy Research, Konkoly-Thege M. u. 29-33, H-1121 Budapest, HungaryNanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Centre for Energy Research, Konkoly-Thege M. u. 29-33, H-1121 Budapest, HungaryNanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Centre for Energy Research, Konkoly-Thege M. u. 29-33, H-1121 Budapest, HungaryAgro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman Ottó u. 15, H-1022 Budapest, Hungary; Corresponding author.Cytotoxic effects of the market leading broad-spectrum, synthetic herbicide product Roundup Classic, its active ingredient glyphosate (in a form of its isopropylamine (IPA) salt) and its formulating surfactant polyethoxylated tallowamine (POE-15) were determined on two murine cell lines, a neuroectodermal stem cell-like (NE-4C) and a high alkaline phosphatase activity osteoblastic cell line (MC3T3-E1). Cytotoxicity, genotoxicity, effects on cell viability and cell cycles were examined in five flow cytometry tests, the two former of which were compared by the enzymatic-assay and the alkaline single cell gel electrophoresis (Comet) assay. All of the tests indicated the NE-4C cells being more sensitive, than the MC3T3-E1 cell line to the treatments with the target compounds. Higher sensitivity differences were detected in the viability test by flow cytometry (7–9-fold), than by the MTT assay (1.5–3-fold); in the genotoxicity test by the Comet assay (3.5–403-fold), than by the DNA-damage test (9.3–158-fold); and in the apoptosis test by the Annexin V dead cell kit (1.1–12.7-fold), than by the Caspase 3/7 kit (1–6.5-fold). Cell cycle assays indicated high count of cells (~70%) in the G0/G1 phase for MC3T3-E1 cells, than in NE-4C cell (~40%) after 24 h. The order of the inhibitory potency of the target substances has unequivocally been POE-15 > Roundup Classic > > glyphosate IPA salt.http://www.sciencedirect.com/science/article/pii/S221475002200097XCell viabilityApoptosisCell cycleDNA damageGlyphosateCo-formulant |
| spellingShingle | Marianna Oláh Enikő Farkas Inna Székács Robert Horvath András Székács Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays Toxicology Reports Cell viability Apoptosis Cell cycle DNA damage Glyphosate Co-formulant |
| title | Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays |
| title_full | Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays |
| title_fullStr | Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays |
| title_full_unstemmed | Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays |
| title_short | Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays |
| title_sort | cytotoxic effects of roundup classic and its components on ne 4c and mc3t3 e1 cell lines determined by biochemical and flow cytometric assays |
| topic | Cell viability Apoptosis Cell cycle DNA damage Glyphosate Co-formulant |
| url | http://www.sciencedirect.com/science/article/pii/S221475002200097X |
| work_keys_str_mv | AT mariannaolah cytotoxiceffectsofroundupclassicanditscomponentsonne4candmc3t3e1celllinesdeterminedbybiochemicalandflowcytometricassays AT enikofarkas cytotoxiceffectsofroundupclassicanditscomponentsonne4candmc3t3e1celllinesdeterminedbybiochemicalandflowcytometricassays AT innaszekacs cytotoxiceffectsofroundupclassicanditscomponentsonne4candmc3t3e1celllinesdeterminedbybiochemicalandflowcytometricassays AT roberthorvath cytotoxiceffectsofroundupclassicanditscomponentsonne4candmc3t3e1celllinesdeterminedbybiochemicalandflowcytometricassays AT andrasszekacs cytotoxiceffectsofroundupclassicanditscomponentsonne4candmc3t3e1celllinesdeterminedbybiochemicalandflowcytometricassays |