Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM

Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvatur...

Full description

Bibliographic Details
Main Authors: Isabell Begemann, Ulrike Keller, Harald Nüsse, Jürgen Klingauf, Milos Galic
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Cells
Subjects:
Online Access:https://www.mdpi.com/2073-4409/9/6/1329