Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis
Insecticide-based vector control measures play an important role in the prevention and control of insect-borne infectious diseases such as malaria; however, insecticide resistance has become a severe global problem for vector control. To date, the metabolic mechanism by which Anopheles sinensis, the...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322003931 |
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| author | Yueyue Li Yashu Li Guanxi Wang Julin Li Meihua Zhang Jingyao Wu Cheng Liang Huayun Zhou Jianxia Tang Guoding Zhu |
| author_facet | Yueyue Li Yashu Li Guanxi Wang Julin Li Meihua Zhang Jingyao Wu Cheng Liang Huayun Zhou Jianxia Tang Guoding Zhu |
| author_sort | Yueyue Li |
| collection | DOAJ |
| description | Insecticide-based vector control measures play an important role in the prevention and control of insect-borne infectious diseases such as malaria; however, insecticide resistance has become a severe global problem for vector control. To date, the metabolic mechanism by which Anopheles sinensis, the most widely distributed malaria vector in China and Asia, detoxifies insecticides is not clear. In this study, the molecular metabolite changes in both the larval and adult stages of deltamethrin susceptible (DS) and deltamethrin-resistant (DR) An. sinensis mosquitoes were analysed by using liquid chromatography tandem mass spectrometry (LC-MS/MS) after exposure to deltamethrin. There were 127 differential metabolites in larval DR An. sinensis and 168 in adults. Five metabolites (glycerophosphocholine, deoxyguanosine, DL-methionine sulfoxide, D-myo-inositol-3-phosphate and N-acetyl-alpha-D-glucosamine1-phosphate) were downregulated in both DR larvae and adults, and one metabolite (aspartyl-glutamine) was upregulated, and the ratio of down- and up-regulation of these metabolites was 5:1. The differential metabolites between the DS and DR mosquitos were mainly classified into organic oxygen compounds, carboxylic acids and their derivatives, glycerophospholipids and purine nucleotides, and the common pathway enriched in both the larval and adult DR An. sinensis was glycerophospholipid metabolism. The findings of this study provide further mechanistic understanding of insecticide resistance in An. sinensis. |
| first_indexed | 2024-12-14T18:52:49Z |
| format | Article |
| id | doaj.art-9accb0b678244138a8f6d3425f6bf531 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-14T18:52:49Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-9accb0b678244138a8f6d3425f6bf5312022-12-21T22:51:11ZengElsevierEcotoxicology and Environmental Safety0147-65132022-06-01237113553Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensisYueyue Li0Yashu Li1Guanxi Wang2Julin Li3Meihua Zhang4Jingyao Wu5Cheng Liang6Huayun Zhou7Jianxia Tang8Guoding Zhu9National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Corresponding authors at: National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaNational Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Corresponding authors at: National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, ChinaInsecticide-based vector control measures play an important role in the prevention and control of insect-borne infectious diseases such as malaria; however, insecticide resistance has become a severe global problem for vector control. To date, the metabolic mechanism by which Anopheles sinensis, the most widely distributed malaria vector in China and Asia, detoxifies insecticides is not clear. In this study, the molecular metabolite changes in both the larval and adult stages of deltamethrin susceptible (DS) and deltamethrin-resistant (DR) An. sinensis mosquitoes were analysed by using liquid chromatography tandem mass spectrometry (LC-MS/MS) after exposure to deltamethrin. There were 127 differential metabolites in larval DR An. sinensis and 168 in adults. Five metabolites (glycerophosphocholine, deoxyguanosine, DL-methionine sulfoxide, D-myo-inositol-3-phosphate and N-acetyl-alpha-D-glucosamine1-phosphate) were downregulated in both DR larvae and adults, and one metabolite (aspartyl-glutamine) was upregulated, and the ratio of down- and up-regulation of these metabolites was 5:1. The differential metabolites between the DS and DR mosquitos were mainly classified into organic oxygen compounds, carboxylic acids and their derivatives, glycerophospholipids and purine nucleotides, and the common pathway enriched in both the larval and adult DR An. sinensis was glycerophospholipid metabolism. The findings of this study provide further mechanistic understanding of insecticide resistance in An. sinensis.http://www.sciencedirect.com/science/article/pii/S0147651322003931Anopheles sinensisDeltamethrinInsecticide resistanceLC-MS/MSMetabolomics |
| spellingShingle | Yueyue Li Yashu Li Guanxi Wang Julin Li Meihua Zhang Jingyao Wu Cheng Liang Huayun Zhou Jianxia Tang Guoding Zhu Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis Ecotoxicology and Environmental Safety Anopheles sinensis Deltamethrin Insecticide resistance LC-MS/MS Metabolomics |
| title | Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis |
| title_full | Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis |
| title_fullStr | Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis |
| title_full_unstemmed | Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis |
| title_short | Differential metabolome responses to deltamethrin between resistant and susceptible Anopheles sinensis |
| title_sort | differential metabolome responses to deltamethrin between resistant and susceptible anopheles sinensis |
| topic | Anopheles sinensis Deltamethrin Insecticide resistance LC-MS/MS Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322003931 |
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