Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material
Despite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/9/764 |
| _version_ | 1827705952003948544 |
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| author | Kymora B Scotland Sonia HY Kung Ben H Chew Dirk Lange |
| author_facet | Kymora B Scotland Sonia HY Kung Ben H Chew Dirk Lange |
| author_sort | Kymora B Scotland |
| collection | DOAJ |
| description | Despite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating bacterial adhesion and biofilm formation remains largely unknown. Here, we examined the interaction of previously identified stent-bound conditioning film components (fibrinogen, uromodulin, and albumin) with bacterial uropathogens. Cytoscopically removed stents were incubated with common uropathogens (<i>Escherichia coli</i>, <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>). Immunofluorescent double staining was performed to study the localization of uropathogens relative to stent-bound conditioning film proteins. Conditioning film components were identified on the external stent surface with some deposition in the inner lumen. Bacteria co-localized with fibrinogen, uromodulin, and albumin, suggesting a potential mechanism for stent-associated infections. Here, we determine strong co-localization between common uropathogenic bacterial species with prominent conditioning film components on ureteral stents. Further functional validation of interactions amongst these uropathogens and conditioning film proteins may enhance clinical management for stent-associated infections and development of improved stent technologies. |
| first_indexed | 2024-03-10T16:13:46Z |
| format | Article |
| id | doaj.art-9acccec52f16486b8e3b34b4b5ff164c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T16:13:46Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-9acccec52f16486b8e3b34b4b5ff164c2023-11-20T14:14:37ZengMDPI AGPathogens2076-08172020-09-019976410.3390/pathogens9090764Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent MaterialKymora B Scotland0Sonia HY Kung1Ben H Chew2Dirk Lange3Department of Urology, University of California Los Angeles, Los Angeles, CA 90024, USAVancouver Prostate Centre, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, CanadaThe Stone Centre at Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, CanadaThe Stone Centre at Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, CanadaDespite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating bacterial adhesion and biofilm formation remains largely unknown. Here, we examined the interaction of previously identified stent-bound conditioning film components (fibrinogen, uromodulin, and albumin) with bacterial uropathogens. Cytoscopically removed stents were incubated with common uropathogens (<i>Escherichia coli</i>, <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>). Immunofluorescent double staining was performed to study the localization of uropathogens relative to stent-bound conditioning film proteins. Conditioning film components were identified on the external stent surface with some deposition in the inner lumen. Bacteria co-localized with fibrinogen, uromodulin, and albumin, suggesting a potential mechanism for stent-associated infections. Here, we determine strong co-localization between common uropathogenic bacterial species with prominent conditioning film components on ureteral stents. Further functional validation of interactions amongst these uropathogens and conditioning film proteins may enhance clinical management for stent-associated infections and development of improved stent technologies.https://www.mdpi.com/2076-0817/9/9/764urinary tract infectionureteral stentbiofilm |
| spellingShingle | Kymora B Scotland Sonia HY Kung Ben H Chew Dirk Lange Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material Pathogens urinary tract infection ureteral stent biofilm |
| title | Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material |
| title_full | Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material |
| title_fullStr | Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material |
| title_full_unstemmed | Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material |
| title_short | Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material |
| title_sort | uropathogens preferrentially interact with conditioning film components on the surface of indwelling ureteral stents rather than stent material |
| topic | urinary tract infection ureteral stent biofilm |
| url | https://www.mdpi.com/2076-0817/9/9/764 |
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