A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection
Summary: Prompt diagnosis is essential for managing herpes simplex virus types 1 and 2 (HSV-1/2). Existing diagnostic methods are not widely available that required expensive or additional equipment for conducting examinations and result readouts, which can limit their utility in resource-constraine...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223026585 |
| _version_ | 1797398577444552704 |
|---|---|
| author | Xiaona Yin Hao Luo Han Zhou Ziyan Zhang Yinyuan Lan Zhanqin Feng Wentao Chen Heping Zheng |
| author_facet | Xiaona Yin Hao Luo Han Zhou Ziyan Zhang Yinyuan Lan Zhanqin Feng Wentao Chen Heping Zheng |
| author_sort | Xiaona Yin |
| collection | DOAJ |
| description | Summary: Prompt diagnosis is essential for managing herpes simplex virus types 1 and 2 (HSV-1/2). Existing diagnostic methods are not widely available that required expensive or additional equipment for conducting examinations and result readouts, which can limit their utility in resource-constrained settings. We successfully developed a CRISPR-Cas13a-based assay for the detection and genotyping of HSV. Our assay demonstrated a high sensitivity of 96.15% and 95.15% for HSV-1 and HSV-2, respectively, with a specificity of 100% compared to a commercial qPCR assay when tested on 194 clinical samples. Remarkably, the assay enables a limit of detection of 1 copy/μL of viral DNA, facilitated by an enhanced input of RPA product and is designed for both mobile app integration and colorimetric interpretation, allowing for semiquantitative readings. These findings highlight the excellent performance of our CRISPR-based diagnostic in detecting HSV and its potential for point-of-care testing in resource-constrained settings. |
| first_indexed | 2024-03-09T01:27:30Z |
| format | Article |
| id | doaj.art-9ad1b53753924458bb92b7763f499109 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-09T01:27:30Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9ad1b53753924458bb92b7763f4991092023-12-10T06:16:58ZengElsevieriScience2589-00422024-01-01271108581A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infectionXiaona Yin0Hao Luo1Han Zhou2Ziyan Zhang3Yinyuan Lan4Zhanqin Feng5Wentao Chen6Heping Zheng7Dermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, China; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China; Corresponding authorDermatology Hospital, Southern Medical University, Guangzhou 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou 510091, China; Corresponding authorSummary: Prompt diagnosis is essential for managing herpes simplex virus types 1 and 2 (HSV-1/2). Existing diagnostic methods are not widely available that required expensive or additional equipment for conducting examinations and result readouts, which can limit their utility in resource-constrained settings. We successfully developed a CRISPR-Cas13a-based assay for the detection and genotyping of HSV. Our assay demonstrated a high sensitivity of 96.15% and 95.15% for HSV-1 and HSV-2, respectively, with a specificity of 100% compared to a commercial qPCR assay when tested on 194 clinical samples. Remarkably, the assay enables a limit of detection of 1 copy/μL of viral DNA, facilitated by an enhanced input of RPA product and is designed for both mobile app integration and colorimetric interpretation, allowing for semiquantitative readings. These findings highlight the excellent performance of our CRISPR-based diagnostic in detecting HSV and its potential for point-of-care testing in resource-constrained settings.http://www.sciencedirect.com/science/article/pii/S2589004223026585Public healthDiagnosticsGenetic engineering |
| spellingShingle | Xiaona Yin Hao Luo Han Zhou Ziyan Zhang Yinyuan Lan Zhanqin Feng Wentao Chen Heping Zheng A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection iScience Public health Diagnostics Genetic engineering |
| title | A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection |
| title_full | A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection |
| title_fullStr | A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection |
| title_full_unstemmed | A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection |
| title_short | A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection |
| title_sort | rapid isothermal crispr cas13a diagnostic test for genital herpes simplex virus infection |
| topic | Public health Diagnostics Genetic engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223026585 |
| work_keys_str_mv | AT xiaonayin arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT haoluo arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT hanzhou arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT ziyanzhang arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT yinyuanlan arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT zhanqinfeng arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT wentaochen arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT hepingzheng arapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT xiaonayin rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT haoluo rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT hanzhou rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT ziyanzhang rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT yinyuanlan rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT zhanqinfeng rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT wentaochen rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection AT hepingzheng rapidisothermalcrisprcas13adiagnostictestforgenitalherpessimplexvirusinfection |