UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet
In recent years, animal husbandry has aimed at improving the conditions of livestock animals useful for humans to solve environmental and health problems. The formulation of animal feeds or supplements based on antioxidant plant compounds is considered a valuable approach and an alternative for live...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/7/2217 |
| _version_ | 1797438398370152448 |
|---|---|
| author | Marialuisa Formato Simona Piccolella Christian Zidorn Alessandro Vastolo Serena Calabrò Monica Isabella Cutrignelli Severina Pacifico |
| author_facet | Marialuisa Formato Simona Piccolella Christian Zidorn Alessandro Vastolo Serena Calabrò Monica Isabella Cutrignelli Severina Pacifico |
| author_sort | Marialuisa Formato |
| collection | DOAJ |
| description | In recent years, animal husbandry has aimed at improving the conditions of livestock animals useful for humans to solve environmental and health problems. The formulation of animal feeds or supplements based on antioxidant plant compounds is considered a valuable approach and an alternative for livestock productivity. Forest biomass materials are an underestimated source of polyphenolic compounds whose sustainable recovery could provide direct benefits to animals and, indirectly, human nutrition. In this context, an alcohol extract from leaves of <i>Fagus sylvatica</i> L. was first investigated through an untargeted ultra-high-performance liquid chromatography–high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) approach. Then, it was fractionated into a fatty acid-rich and a polyphenolic fraction, as evidenced by total lipid, phenol, and flavonoid content assays, with antiradical and reducing activity positively correlated to the latter. When tested in vitro with rumen liquor to evaluate changes in the fermentative parameters, a significant detrimental effect was exerted by the lipid-rich fraction, whereas the flavonoid-rich one positively modulated the production of volatile fatty acids (i.e., acetate, butyrate, propionate, etc.). |
| first_indexed | 2024-03-09T11:37:20Z |
| format | Article |
| id | doaj.art-9ad28b182ecb417ba200053325d6e422 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:37:20Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9ad28b182ecb417ba200053325d6e4222023-11-30T23:41:12ZengMDPI AGMolecules1420-30492022-03-01277221710.3390/molecules27072217UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant DietMarialuisa Formato0Simona Piccolella1Christian Zidorn2Alessandro Vastolo3Serena Calabrò4Monica Isabella Cutrignelli5Severina Pacifico6Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania ‘Luigi Vanvitelli’, Via Vivaldi 43, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania ‘Luigi Vanvitelli’, Via Vivaldi 43, 81100 Caserta, ItalyPharmazeutisches Institut, Abteilung Pharmazeutische Biologie, Christian-Albrechts-Universität zu Kiel, Gutenbergstraße 76, 24118 Kiel, GermanyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137 Napoli, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137 Napoli, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137 Napoli, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania ‘Luigi Vanvitelli’, Via Vivaldi 43, 81100 Caserta, ItalyIn recent years, animal husbandry has aimed at improving the conditions of livestock animals useful for humans to solve environmental and health problems. The formulation of animal feeds or supplements based on antioxidant plant compounds is considered a valuable approach and an alternative for livestock productivity. Forest biomass materials are an underestimated source of polyphenolic compounds whose sustainable recovery could provide direct benefits to animals and, indirectly, human nutrition. In this context, an alcohol extract from leaves of <i>Fagus sylvatica</i> L. was first investigated through an untargeted ultra-high-performance liquid chromatography–high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) approach. Then, it was fractionated into a fatty acid-rich and a polyphenolic fraction, as evidenced by total lipid, phenol, and flavonoid content assays, with antiradical and reducing activity positively correlated to the latter. When tested in vitro with rumen liquor to evaluate changes in the fermentative parameters, a significant detrimental effect was exerted by the lipid-rich fraction, whereas the flavonoid-rich one positively modulated the production of volatile fatty acids (i.e., acetate, butyrate, propionate, etc.).https://www.mdpi.com/1420-3049/27/7/2217<i>Fagus sylvatica</i> L.UHPLC-ESI-Q<i>q</i>TOF analysispolyphenolsflavonoidsin vitro rumen fermentationvolatile fatty acids |
| spellingShingle | Marialuisa Formato Simona Piccolella Christian Zidorn Alessandro Vastolo Serena Calabrò Monica Isabella Cutrignelli Severina Pacifico UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet Molecules <i>Fagus sylvatica</i> L. UHPLC-ESI-Q<i>q</i>TOF analysis polyphenols flavonoids in vitro rumen fermentation volatile fatty acids |
| title | UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet |
| title_full | UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet |
| title_fullStr | UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet |
| title_full_unstemmed | UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet |
| title_short | UHPLC-ESI-Q<i>q</i>TOF Analysis and In Vitro Rumen Fermentation for Exploiting <i>Fagus sylvatica</i> Leaf in Ruminant Diet |
| title_sort | uhplc esi q i q i tof analysis and in vitro rumen fermentation for exploiting i fagus sylvatica i leaf in ruminant diet |
| topic | <i>Fagus sylvatica</i> L. UHPLC-ESI-Q<i>q</i>TOF analysis polyphenols flavonoids in vitro rumen fermentation volatile fatty acids |
| url | https://www.mdpi.com/1420-3049/27/7/2217 |
| work_keys_str_mv | AT marialuisaformato uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT simonapiccolella uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT christianzidorn uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT alessandrovastolo uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT serenacalabro uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT monicaisabellacutrignelli uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet AT severinapacifico uhplcesiqiqitofanalysisandinvitrorumenfermentationforexploitingifagussylvaticaileafinruminantdiet |