The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut
Background Low-temperatures have the potential to be a serious problem for plants and can negatively affect the normal growth and development of walnuts. DREB1/CBF (Dehydration Responsive Element Binding Protein 1/C-repeat Binding Factor), one of the most direct transcription factors in response to...
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PeerJ Inc.
2022-09-01
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| author | Liqun Han Kai Ma Yu Zhao Chuang Mei Aisajan Mamat Jixun Wang Ling Qin Tianming He |
| author_facet | Liqun Han Kai Ma Yu Zhao Chuang Mei Aisajan Mamat Jixun Wang Ling Qin Tianming He |
| author_sort | Liqun Han |
| collection | DOAJ |
| description | Background Low-temperatures have the potential to be a serious problem for plants and can negatively affect the normal growth and development of walnuts. DREB1/CBF (Dehydration Responsive Element Binding Protein 1/C-repeat Binding Factor), one of the most direct transcription factors in response to low-temperature stress, may improve the resistance of plants to low-temperatures by regulating their functional genes. However, few studies have been conducted in walnut. The Xinjiang wild walnut is a rare wild plant found in China, with a large number of excellent trait genes, and is hardier than cultivated walnuts in Xinjiang. Methods In this work, we identified all of the DREB1 members from the walnut genome and analyzed their expression levels in different tissues and during low-temperature stress on the Xinjiang wild walnut. The JfDREB1A gene of the Xinjiang wild walnut was cloned and transformed into Arabidopsis thaliana for functional verification. Results There were five DREB1 transcription factors in the walnut genome. Among them, the relative expression level of the DREB1A gene was significantly higher than other members in the different tissues (root, stem, leaf) and was immediately un-regulated under low-temperature stress. The overexpression of the JfDREB1A gene increased the survival rates of transgenic Arabidopsis lines, mainly through maintaining the stability of cell membrane, decreasing the electrical conductivity and increasing the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the expression levels of cold-inducible genes like AtKIN1, AtERD10, AtRD29A, AtCOR15A and AtCOR47, were significantly increased. These results showed that the JfDREB1A gene may play an important role in the response to cold stress of the Xinjiang wild walnut. This study contributes to our understanding of the molecular mechanism of the Xinjiang wild walnut’s response to low-temperature stress and will be beneficial for developing walnut cultivars with improved cold resistance. |
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| spelling | doaj.art-9ad3454707054b7ab19a2faf8d3be7a02023-12-02T21:50:08ZengPeerJ Inc.PeerJ2167-83592022-09-0110e1402110.7717/peerj.14021The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnutLiqun Han0Kai Ma1Yu Zhao2Chuang Mei3Aisajan Mamat4Jixun Wang5Ling Qin6Tianming He7College of Horticulture, Xinjiang Agricultural University, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables/Xinjiang Fruit Science Experiment Station, Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables/Xinjiang Fruit Science Experiment Station, Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables/Xinjiang Fruit Science Experiment Station, Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables/Xinjiang Fruit Science Experiment Station, Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables/Xinjiang Fruit Science Experiment Station, Ministry of Agriculture, Urumqi, ChinaCollege of Horticulture, Xinjiang Agricultural University, Urumqi, ChinaCollege of Horticulture, Xinjiang Agricultural University, Urumqi, ChinaBackground Low-temperatures have the potential to be a serious problem for plants and can negatively affect the normal growth and development of walnuts. DREB1/CBF (Dehydration Responsive Element Binding Protein 1/C-repeat Binding Factor), one of the most direct transcription factors in response to low-temperature stress, may improve the resistance of plants to low-temperatures by regulating their functional genes. However, few studies have been conducted in walnut. The Xinjiang wild walnut is a rare wild plant found in China, with a large number of excellent trait genes, and is hardier than cultivated walnuts in Xinjiang. Methods In this work, we identified all of the DREB1 members from the walnut genome and analyzed their expression levels in different tissues and during low-temperature stress on the Xinjiang wild walnut. The JfDREB1A gene of the Xinjiang wild walnut was cloned and transformed into Arabidopsis thaliana for functional verification. Results There were five DREB1 transcription factors in the walnut genome. Among them, the relative expression level of the DREB1A gene was significantly higher than other members in the different tissues (root, stem, leaf) and was immediately un-regulated under low-temperature stress. The overexpression of the JfDREB1A gene increased the survival rates of transgenic Arabidopsis lines, mainly through maintaining the stability of cell membrane, decreasing the electrical conductivity and increasing the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the expression levels of cold-inducible genes like AtKIN1, AtERD10, AtRD29A, AtCOR15A and AtCOR47, were significantly increased. These results showed that the JfDREB1A gene may play an important role in the response to cold stress of the Xinjiang wild walnut. This study contributes to our understanding of the molecular mechanism of the Xinjiang wild walnut’s response to low-temperature stress and will be beneficial for developing walnut cultivars with improved cold resistance.https://peerj.com/articles/14021.pdfXinjiang wild walnutJfDREB1A geneLow-temperature stressTransgenic ArabidopsisCold resistance |
| spellingShingle | Liqun Han Kai Ma Yu Zhao Chuang Mei Aisajan Mamat Jixun Wang Ling Qin Tianming He The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut PeerJ Xinjiang wild walnut JfDREB1A gene Low-temperature stress Transgenic Arabidopsis Cold resistance |
| title | The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut |
| title_full | The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut |
| title_fullStr | The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut |
| title_full_unstemmed | The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut |
| title_short | The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut |
| title_sort | cold stress responsive gene dreb1a involved in low temperature tolerance in xinjiang wild walnut |
| topic | Xinjiang wild walnut JfDREB1A gene Low-temperature stress Transgenic Arabidopsis Cold resistance |
| url | https://peerj.com/articles/14021.pdf |
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