Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds
Bovine herpesvirus type 4 (BoHV‑4) is a common virus in the world that is detected in clinically ill or in apparently healthy cattle. This study provides a molecular characterization of BoHV‑4 strains from 24 cattle some showing respiratory and/or reproductive problems and some without any apparent...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2021-05-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2095 |
| _version_ | 1828882668959301632 |
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| author | Seval Bilge Dağalp Firat Dogan Ali Riza Babaoglu Touraj Aligholipour Farzani Feray Alkan |
| author_facet | Seval Bilge Dağalp Firat Dogan Ali Riza Babaoglu Touraj Aligholipour Farzani Feray Alkan |
| author_sort | Seval Bilge Dağalp |
| collection | DOAJ |
| description | Bovine herpesvirus type 4 (BoHV‑4) is a common virus in the world that is detected in clinically ill or in apparently healthy cattle. This study provides a molecular characterization of BoHV‑4 strains from 24 cattle some showing respiratory and/or reproductive problems and some without any apparent clinical sign. This study also reported the growth properties of five BoHV‑4 field isolates. The 24 sampled cattle came from 13 different herds in 10 provinces collected between 2007 and 2018. Phylogenetic analysis using partially amplified nucleotide sequences of ORF8 genes coding glycoprotein B (n = 24) and ORF3 genes coding thymidine kinase (n = 9), demonstrated genetic variability among the BoHV‑4 strains analysed. The partial gB gene sequences clustered in three different genotypes (genotype I, II and III) were located within the genotype I cluster, such as Movar strain. The analysis of the five BoHV‑4 strains isolated from vaginal swabs (n = 2), nasal swab (n = 1), and brain samples (n = 2) revealed no significant differences in their growth properties in MDBK cell culture. |
| first_indexed | 2024-12-13T10:32:43Z |
| format | Article |
| id | doaj.art-9ad66deaf9884caeaaed5a2cf2fb4b13 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-13T10:32:43Z |
| publishDate | 2021-05-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-9ad66deaf9884caeaaed5a2cf2fb4b132022-12-21T23:50:49ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272021-05-0157110.12834/VetIt.2095.11150.1Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herdsSeval Bilge Dağalp0Firat Dogan1Ali Riza Babaoglu2Touraj Aligholipour Farzani3Feray Alkan4Virology Department, Ankara University, Veterinary Medicine, Ankara, TurkeyVirology Departmen, Hatay Mustafa Kemal University, Veterinary Medicine, Hatay, TurkeyVirology Department, Van Yuzuncuyıl University, Veterinary Medicine, Van TurkeyVirology Department, Ankara University, Veterinary Medicine, Ankara, TurkeyVirology Department, Ankara University, Veterinary Medicine, Ankara, TurkeyBovine herpesvirus type 4 (BoHV‑4) is a common virus in the world that is detected in clinically ill or in apparently healthy cattle. This study provides a molecular characterization of BoHV‑4 strains from 24 cattle some showing respiratory and/or reproductive problems and some without any apparent clinical sign. This study also reported the growth properties of five BoHV‑4 field isolates. The 24 sampled cattle came from 13 different herds in 10 provinces collected between 2007 and 2018. Phylogenetic analysis using partially amplified nucleotide sequences of ORF8 genes coding glycoprotein B (n = 24) and ORF3 genes coding thymidine kinase (n = 9), demonstrated genetic variability among the BoHV‑4 strains analysed. The partial gB gene sequences clustered in three different genotypes (genotype I, II and III) were located within the genotype I cluster, such as Movar strain. The analysis of the five BoHV‑4 strains isolated from vaginal swabs (n = 2), nasal swab (n = 1), and brain samples (n = 2) revealed no significant differences in their growth properties in MDBK cell culture.https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2095Bovine Herpesvirus Type 4glycoprotein B (gB)PCRsequencingthymidine kinase (TK)Virus isolation |
| spellingShingle | Seval Bilge Dağalp Firat Dogan Ali Riza Babaoglu Touraj Aligholipour Farzani Feray Alkan Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds Veterinaria Italiana Bovine Herpesvirus Type 4 glycoprotein B (gB) PCR sequencing thymidine kinase (TK) Virus isolation |
| title | Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds |
| title_full | Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds |
| title_fullStr | Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds |
| title_full_unstemmed | Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds |
| title_short | Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds |
| title_sort | genetic variability of bovine herpesvirus type 4 bohv 4 field strains from turkish cattle herds |
| topic | Bovine Herpesvirus Type 4 glycoprotein B (gB) PCR sequencing thymidine kinase (TK) Virus isolation |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2095 |
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