Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan
<p>Abstract</p> <p>A total of 318 <it>Escherichia coli </it>isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolat...
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| Format: | Article |
| Language: | English |
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BMC
2011-10-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://www.actavetscand.com/content/53/1/52 |
| _version_ | 1818675623562838016 |
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| author | Ozawa Manao Baba Kotaro Usui Masaru Hiki Mototaka Sato Chizuru Masani Kaori Asai Tetsuo Harada Kazuki Aoki Hiroshi Sawada Takuo |
| author_facet | Ozawa Manao Baba Kotaro Usui Masaru Hiki Mototaka Sato Chizuru Masani Kaori Asai Tetsuo Harada Kazuki Aoki Hiroshi Sawada Takuo |
| author_sort | Ozawa Manao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>A total of 318 <it>Escherichia coli </it>isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of <it>E. coli </it>from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced <it>bla<sub>CTX-M-2</sub></it>, while the nine poultry isolates produced <it>bla<sub>CTX-M-25 </sub></it>(4), <it>bla<sub>SHV-2 </sub></it>(3), <it>bla<sub>CTX-M-15 </sub></it>(1) and <it>bla<sub>CTX-M-2 </sub></it>(1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in <it>E. coli </it>from diseased animals in Japan.</p> |
| first_indexed | 2024-12-17T08:30:32Z |
| format | Article |
| id | doaj.art-9ad6a0539ae54e0a946e8b002e762de2 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-17T08:30:32Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-9ad6a0539ae54e0a946e8b002e762de22022-12-21T21:56:37ZengBMCActa Veterinaria Scandinavica1751-01472011-10-015315210.1186/1751-0147-53-52Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in JapanOzawa ManaoBaba KotaroUsui MasaruHiki MototakaSato ChizuruMasani KaoriAsai TetsuoHarada KazukiAoki HiroshiSawada Takuo<p>Abstract</p> <p>A total of 318 <it>Escherichia coli </it>isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of <it>E. coli </it>from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced <it>bla<sub>CTX-M-2</sub></it>, while the nine poultry isolates produced <it>bla<sub>CTX-M-25 </sub></it>(4), <it>bla<sub>SHV-2 </sub></it>(3), <it>bla<sub>CTX-M-15 </sub></it>(1) and <it>bla<sub>CTX-M-2 </sub></it>(1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in <it>E. coli </it>from diseased animals in Japan.</p>http://www.actavetscand.com/content/53/1/52<it>Escherichia coli</it>food-producing animalscephalosporin resistanceextended spectrum β-lactamaseAmpC β-lactamasephylogenetic grouping |
| spellingShingle | Ozawa Manao Baba Kotaro Usui Masaru Hiki Mototaka Sato Chizuru Masani Kaori Asai Tetsuo Harada Kazuki Aoki Hiroshi Sawada Takuo Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan Acta Veterinaria Scandinavica <it>Escherichia coli</it> food-producing animals cephalosporin resistance extended spectrum β-lactamase AmpC β-lactamase phylogenetic grouping |
| title | Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan |
| title_full | Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan |
| title_fullStr | Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan |
| title_full_unstemmed | Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan |
| title_short | Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan |
| title_sort | phylogenetic groups and cephalosporin resistance genes of it escherichia coli it from diseased food producing animals in japan |
| topic | <it>Escherichia coli</it> food-producing animals cephalosporin resistance extended spectrum β-lactamase AmpC β-lactamase phylogenetic grouping |
| url | http://www.actavetscand.com/content/53/1/52 |
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