The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer
Intratumor morphological heterogeneity reflects patterns of invasive growth and is an indicator of the metastatic potential of breast cancer. In this study, we used this heterogeneity to identify molecules associated with breast cancer invasion and metastasis. The gene expression microarray data wer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/7/1909 |
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| author | Tatiana S. Gerashchenko Sofia Y. Zolotaryova Artem M. Kiselev Liubov A. Tashireva Nikita M. Novikov Nadezhda V. Krakhmal Nadezhda V. Cherdyntseva Marina V. Zavyalova Vladimir M. Perelmuter Evgeny V. Denisov |
| author_facet | Tatiana S. Gerashchenko Sofia Y. Zolotaryova Artem M. Kiselev Liubov A. Tashireva Nikita M. Novikov Nadezhda V. Krakhmal Nadezhda V. Cherdyntseva Marina V. Zavyalova Vladimir M. Perelmuter Evgeny V. Denisov |
| author_sort | Tatiana S. Gerashchenko |
| collection | DOAJ |
| description | Intratumor morphological heterogeneity reflects patterns of invasive growth and is an indicator of the metastatic potential of breast cancer. In this study, we used this heterogeneity to identify molecules associated with breast cancer invasion and metastasis. The gene expression microarray data were used to identify genes differentially expressed between solid, trabecular, and other morphological arrangements of tumor cells. Immunohistochemistry was applied to evaluate the association of the selected proteins with metastasis. RNA-sequencing was performed to analyze the molecular makeup of metastatic tumor cells. High frequency of metastases and decreased metastasis-free survival were detected in patients either with positive expression of KIF14 or Mieap or negative expression of EZR at the tips of the torpedo-like structures in breast cancers. KIF14- and Mieap-positive and EZR-negative cells were mainly detected in the torpedo-like structures of the same breast tumors; however, their transcriptomic features differed. KIF14-positive cells showed a significant upregulation of genes involved in ether lipid metabolism. Mieap-positive cells were enriched in genes involved in mitophagy. EZR-negative cells displayed upregulated genes associated with phagocytosis and the chemokine-mediated signaling pathway. In conclusion, the positive expression of KIF14 and Mieap and negative expression of EZR at the tips of the torpedo-like structures are associated with breast cancer metastasis. |
| first_indexed | 2024-03-10T18:27:49Z |
| format | Article |
| id | doaj.art-9ad702c7133248e4aa0a01b582656cd0 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T18:27:49Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-9ad702c7133248e4aa0a01b582656cd02023-11-20T06:51:16ZengMDPI AGCancers2072-66942020-07-01127190910.3390/cancers12071909The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast CancerTatiana S. Gerashchenko0Sofia Y. Zolotaryova1Artem M. Kiselev2Liubov A. Tashireva3Nikita M. Novikov4Nadezhda V. Krakhmal5Nadezhda V. Cherdyntseva6Marina V. Zavyalova7Vladimir M. Perelmuter8Evgeny V. Denisov9Laboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaLaboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaLaboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaDepartment of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaLaboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaDepartment of Pathological Anatomy, Siberian State Medical University, 634050 Tomsk, RussiaLaboratory of Molecular Oncology and Immunology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaDepartment of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaDepartment of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaLaboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, RussiaIntratumor morphological heterogeneity reflects patterns of invasive growth and is an indicator of the metastatic potential of breast cancer. In this study, we used this heterogeneity to identify molecules associated with breast cancer invasion and metastasis. The gene expression microarray data were used to identify genes differentially expressed between solid, trabecular, and other morphological arrangements of tumor cells. Immunohistochemistry was applied to evaluate the association of the selected proteins with metastasis. RNA-sequencing was performed to analyze the molecular makeup of metastatic tumor cells. High frequency of metastases and decreased metastasis-free survival were detected in patients either with positive expression of KIF14 or Mieap or negative expression of EZR at the tips of the torpedo-like structures in breast cancers. KIF14- and Mieap-positive and EZR-negative cells were mainly detected in the torpedo-like structures of the same breast tumors; however, their transcriptomic features differed. KIF14-positive cells showed a significant upregulation of genes involved in ether lipid metabolism. Mieap-positive cells were enriched in genes involved in mitophagy. EZR-negative cells displayed upregulated genes associated with phagocytosis and the chemokine-mediated signaling pathway. In conclusion, the positive expression of KIF14 and Mieap and negative expression of EZR at the tips of the torpedo-like structures are associated with breast cancer metastasis.https://www.mdpi.com/2072-6694/12/7/1909breast cancermetastasisinvasionheterogeneitymorphologytorpedo-like structures |
| spellingShingle | Tatiana S. Gerashchenko Sofia Y. Zolotaryova Artem M. Kiselev Liubov A. Tashireva Nikita M. Novikov Nadezhda V. Krakhmal Nadezhda V. Cherdyntseva Marina V. Zavyalova Vladimir M. Perelmuter Evgeny V. Denisov The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer Cancers breast cancer metastasis invasion heterogeneity morphology torpedo-like structures |
| title | The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer |
| title_full | The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer |
| title_fullStr | The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer |
| title_full_unstemmed | The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer |
| title_short | The Activity of KIF14, Mieap, and EZR in a New Type of the Invasive Component, Torpedo-Like Structures, Predetermines the Metastatic Potential of Breast Cancer |
| title_sort | activity of kif14 mieap and ezr in a new type of the invasive component torpedo like structures predetermines the metastatic potential of breast cancer |
| topic | breast cancer metastasis invasion heterogeneity morphology torpedo-like structures |
| url | https://www.mdpi.com/2072-6694/12/7/1909 |
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