Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1066543/full |
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| author | Mingzheng Han Mingzheng Han Mingzheng Han Wenfeng Hu Wenfeng Hu Wenfeng Hu Tong Chen Tong Chen Hanxing Guo Hanxing Guo Hanxing Guo Jianfeng Zhu Jianfeng Zhu Feng Chen Feng Chen Feng Chen |
| author_facet | Mingzheng Han Mingzheng Han Mingzheng Han Wenfeng Hu Wenfeng Hu Wenfeng Hu Tong Chen Tong Chen Hanxing Guo Hanxing Guo Hanxing Guo Jianfeng Zhu Jianfeng Zhu Feng Chen Feng Chen Feng Chen |
| author_sort | Mingzheng Han |
| collection | DOAJ |
| description | Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight plant extracts and essential oils and their mixture on Eimeria tenella as potential treatments for coccidial infection. The anticoccidial effects of non-toxic concentrations of Punica granatum L. (0.005 mg/mL), Plantago asiatica L. (0.780 mg/mL), Bidens pilosa L. (0.390 mg/mL), Acalypha australis L. (0.390 mg/mL), Pteris multifida Poir (0.050 mg/mL), and Portulaca oleracea L. sp. Pl. (0.050 mg/mL) extracts; Artemisia argyi Levl. et Vant. (0.010 μL/mL) and Camellia sinensis (L.) O. Ktze (0.050 μL/mL) essential oils; and their mixture (0.500 mL/mL) on Eimeria tenella were determined using cell viability assays, flow cytometry, and in vivo studies. The eight plant extracts and essential oils and their mixture inhibited Eimeria tenella sporozoites from invading chicken embryo fibroblast cells in vitro. The extract and essential oil mixture improved the feed conversion ratio and body weight gain, reduced fecal oocyst excretion, substantially reduced the mortality of Eimeria tenella-infected chickens, and reduced Eimeria tenella-induced cecal damage in vivo. The results suggest that the extract and essential oil mixtures inhibit Eimeria tenella invasion both in vitro and in vivo, demonstrating their potential as anticoccidial agents. |
| first_indexed | 2024-04-11T06:43:55Z |
| format | Article |
| id | doaj.art-9ad8125fc2c548c0af8985439bce01ab |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T06:43:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-9ad8125fc2c548c0af8985439bce01ab2022-12-22T04:39:25ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.10665431066543Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo studyMingzheng Han0Mingzheng Han1Mingzheng Han2Wenfeng Hu3Wenfeng Hu4Wenfeng Hu5Tong Chen6Tong Chen7Hanxing Guo8Hanxing Guo9Hanxing Guo10Jianfeng Zhu11Jianfeng Zhu12Feng Chen13Feng Chen14Feng Chen15College of Animal Science, South China Agricultural University, Guangzhou, ChinaBioforte Biotechnology (Shenzhen) Co., Ltd., Shenzhen, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaBioforte Biotechnology (Shenzhen) Co., Ltd., Shenzhen, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaBioforte Biotechnology (Shenzhen) Co., Ltd., Shenzhen, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaBioforte Biotechnology (Shenzhen) Co., Ltd., Shenzhen, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaBioforte Biotechnology (Shenzhen) Co., Ltd., Shenzhen, ChinaResearch Institute, Wen's Foodstuffs Group Co., Ltd., Yunfu, ChinaCoccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight plant extracts and essential oils and their mixture on Eimeria tenella as potential treatments for coccidial infection. The anticoccidial effects of non-toxic concentrations of Punica granatum L. (0.005 mg/mL), Plantago asiatica L. (0.780 mg/mL), Bidens pilosa L. (0.390 mg/mL), Acalypha australis L. (0.390 mg/mL), Pteris multifida Poir (0.050 mg/mL), and Portulaca oleracea L. sp. Pl. (0.050 mg/mL) extracts; Artemisia argyi Levl. et Vant. (0.010 μL/mL) and Camellia sinensis (L.) O. Ktze (0.050 μL/mL) essential oils; and their mixture (0.500 mL/mL) on Eimeria tenella were determined using cell viability assays, flow cytometry, and in vivo studies. The eight plant extracts and essential oils and their mixture inhibited Eimeria tenella sporozoites from invading chicken embryo fibroblast cells in vitro. The extract and essential oil mixture improved the feed conversion ratio and body weight gain, reduced fecal oocyst excretion, substantially reduced the mortality of Eimeria tenella-infected chickens, and reduced Eimeria tenella-induced cecal damage in vivo. The results suggest that the extract and essential oil mixtures inhibit Eimeria tenella invasion both in vitro and in vivo, demonstrating their potential as anticoccidial agents.https://www.frontiersin.org/articles/10.3389/fvets.2022.1066543/fullanticoccidial agentbroilercoccidiosisEimeria tenellaessential oilplant extract |
| spellingShingle | Mingzheng Han Mingzheng Han Mingzheng Han Wenfeng Hu Wenfeng Hu Wenfeng Hu Tong Chen Tong Chen Hanxing Guo Hanxing Guo Hanxing Guo Jianfeng Zhu Jianfeng Zhu Feng Chen Feng Chen Feng Chen Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study Frontiers in Veterinary Science anticoccidial agent broiler coccidiosis Eimeria tenella essential oil plant extract |
| title | Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study |
| title_full | Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study |
| title_fullStr | Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study |
| title_full_unstemmed | Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study |
| title_short | Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study |
| title_sort | anticoccidial activity of natural plants extracts mixture against eimeria tenella an in vitro and in vivo study |
| topic | anticoccidial agent broiler coccidiosis Eimeria tenella essential oil plant extract |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1066543/full |
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