Process Optimization for the Extraction of Polyphenols from Okara

The objective of the present investigation is to examine okara, a suitable substrate for polyphenol extraction, and to develop a feasible eco-friendly process to maximize the yield of antioxidant phenolics. Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to investig...

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Main Authors: Geetanjali Yadav, Arindam Kuila, Anshu Singh, Rintu Banerjee
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2011-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/105763
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author Geetanjali Yadav
Arindam Kuila
Anshu Singh
Rintu Banerjee
author_facet Geetanjali Yadav
Arindam Kuila
Anshu Singh
Rintu Banerjee
author_sort Geetanjali Yadav
collection DOAJ
description The objective of the present investigation is to examine okara, a suitable substrate for polyphenol extraction, and to develop a feasible eco-friendly process to maximize the yield of antioxidant phenolics. Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to investigate the effect of temperature (°C), solvent fraction (%) and incubation time (min) on polyphenol extraction by using MINITAB 15 software. Acetone was used as solvent to extract the phenolic compounds possessing the antioxidant properties (DPPH radical scavenging activity, reducing power, and metal chelating activity). Extraction under the optimum conditions yielded total polyphenolic content of 1.16 mg/mL, DPPH radical scavenging activity of 61.07 %, metal chelating activity of 61.20 % and better reducing power. The effective model developed for antioxidant mining from okara under mild operational conditions can be a valuable technique for soybean-based food industry.
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spelling doaj.art-9ad948fb2836411896ba27bae1156bb22023-12-02T05:59:01ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062011-01-01493322328Process Optimization for the Extraction of Polyphenols from OkaraGeetanjali Yadav0Arindam Kuila1Anshu Singh2Rintu Banerjee3Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, 721302 Kharagpur, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, 721302 Kharagpur, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, 721302 Kharagpur, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, 721302 Kharagpur, IndiaThe objective of the present investigation is to examine okara, a suitable substrate for polyphenol extraction, and to develop a feasible eco-friendly process to maximize the yield of antioxidant phenolics. Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to investigate the effect of temperature (°C), solvent fraction (%) and incubation time (min) on polyphenol extraction by using MINITAB 15 software. Acetone was used as solvent to extract the phenolic compounds possessing the antioxidant properties (DPPH radical scavenging activity, reducing power, and metal chelating activity). Extraction under the optimum conditions yielded total polyphenolic content of 1.16 mg/mL, DPPH radical scavenging activity of 61.07 %, metal chelating activity of 61.20 % and better reducing power. The effective model developed for antioxidant mining from okara under mild operational conditions can be a valuable technique for soybean-based food industry.http://hrcak.srce.hr/file/105763natural antioxidantspolyphenolicssoybeanokararesponse surface methodology (RSM)
spellingShingle Geetanjali Yadav
Arindam Kuila
Anshu Singh
Rintu Banerjee
Process Optimization for the Extraction of Polyphenols from Okara
Food Technology and Biotechnology
natural antioxidants
polyphenolics
soybean
okara
response surface methodology (RSM)
title Process Optimization for the Extraction of Polyphenols from Okara
title_full Process Optimization for the Extraction of Polyphenols from Okara
title_fullStr Process Optimization for the Extraction of Polyphenols from Okara
title_full_unstemmed Process Optimization for the Extraction of Polyphenols from Okara
title_short Process Optimization for the Extraction of Polyphenols from Okara
title_sort process optimization for the extraction of polyphenols from okara
topic natural antioxidants
polyphenolics
soybean
okara
response surface methodology (RSM)
url http://hrcak.srce.hr/file/105763
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