DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS
Purpose. The routine diagnosis of enterobiasis consists of a microscopic examination of perianal imprints on a transparent scotch tape for the presence of parasitic eggs. However, this method has low sensitivity when the number of eggs is small. The development of highly sensitive and specific metho...
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| Formaat: | Artikel |
| Taal: | English |
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Peytchinski Publishing
2024-01-01
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| Reeks: | Journal of IMAB |
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| Online toegang: | https://www.journal-imab-bg.org/issues-2024/issue1/2024vol30-issue1-5295-5300.pdf |
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| author | Eleonora Kaneva Nina Tsvetkova Desislava Velcheva Rumen Harizanov lskren Kaftandjiev Raina Borisova Mihaela Videnova Aleksandra lvanova Ivailo Alexiev Reneta Dimitrova Maria Pavlova |
| author_facet | Eleonora Kaneva Nina Tsvetkova Desislava Velcheva Rumen Harizanov lskren Kaftandjiev Raina Borisova Mihaela Videnova Aleksandra lvanova Ivailo Alexiev Reneta Dimitrova Maria Pavlova |
| author_sort | Eleonora Kaneva |
| collection | DOAJ |
| description | Purpose. The routine diagnosis of enterobiasis consists of a microscopic examination of perianal imprints on a transparent scotch tape for the presence of parasitic eggs. However, this method has low sensitivity when the number of eggs is small. The development of highly sensitive and specific methods, such as PCR, will increase the possibilities of detecting the parasite and will enable its genetic characterization.
Material/Methods. The study was conducted from May 2022 to September 2022 and comprised 24 patients infected with Enterobius vermicularis. DNA was extracted from parasite eggs/adult worms using PureLink Genomic DNA Mini Kit (INVITROGEN), according to the manufacturer's instructions. Nested polymerase chain reaction (PCR) for detection of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was applied with two sets of primers.
Results. The developed and described method for genetic determination of Enterobius vermicularis in samples from infected individuals is suitable and applicable to improve the diagnosis of the disease, as well as for further phylogenetic research of the parasite, which is of great importance for its successful treatment and control.
Conclusions. Regardless of existing morphological diagnostic methods, genotyping studies concerning Enterobius vermicularis need to be deepened. Molecular-biological analyses increase diagnostic sensitivity, provide valuable knowledge about the geographic distribution and diversity of helminths, and allow the monitoring of therapy. |
| first_indexed | 2024-03-08T14:25:22Z |
| format | Article |
| id | doaj.art-9adbc8441218490ab110fa5fd4087dff |
| institution | Directory Open Access Journal |
| issn | 1312-773X |
| language | English |
| last_indexed | 2024-03-08T14:25:22Z |
| publishDate | 2024-01-01 |
| publisher | Peytchinski Publishing |
| record_format | Article |
| series | Journal of IMAB |
| spelling | doaj.art-9adbc8441218490ab110fa5fd4087dff2024-01-13T05:43:15ZengPeytchinski PublishingJournal of IMAB1312-773X2024-01-013015295530010.5272/jimab.2024301.5295DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSISEleonora Kaneva0https://orcid.org/0000-0003-3869-117XNina Tsvetkova1https://orcid.org/0000-0002-4638-9379Desislava Velcheva2https://orcid.org/0009-0009-4303-6218Rumen Harizanov3https://orcid.org/0000-0003-2745-3344lskren Kaftandjiev4https://orcid.org/0000-0001-7180-6739Raina Borisova5https://orcid.org/0000-0002-2096-9674Mihaela Videnova6https://orcid.org/0000-0003-4019-9395Aleksandra lvanova7https://orcid.org/0000-0002-9984-8639Ivailo Alexiev8https://orcid.org/0000-0002-3186-1124Reneta Dimitrova9https://orcid.org/0000-0001-7711-6798Maria Pavlova10https://orcid.org/0000-0003-2116-6264National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.MDL "Cibalab", Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.Purpose. The routine diagnosis of enterobiasis consists of a microscopic examination of perianal imprints on a transparent scotch tape for the presence of parasitic eggs. However, this method has low sensitivity when the number of eggs is small. The development of highly sensitive and specific methods, such as PCR, will increase the possibilities of detecting the parasite and will enable its genetic characterization. Material/Methods. The study was conducted from May 2022 to September 2022 and comprised 24 patients infected with Enterobius vermicularis. DNA was extracted from parasite eggs/adult worms using PureLink Genomic DNA Mini Kit (INVITROGEN), according to the manufacturer's instructions. Nested polymerase chain reaction (PCR) for detection of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was applied with two sets of primers. Results. The developed and described method for genetic determination of Enterobius vermicularis in samples from infected individuals is suitable and applicable to improve the diagnosis of the disease, as well as for further phylogenetic research of the parasite, which is of great importance for its successful treatment and control. Conclusions. Regardless of existing morphological diagnostic methods, genotyping studies concerning Enterobius vermicularis need to be deepened. Molecular-biological analyses increase diagnostic sensitivity, provide valuable knowledge about the geographic distribution and diversity of helminths, and allow the monitoring of therapy.https://www.journal-imab-bg.org/issues-2024/issue1/2024vol30-issue1-5295-5300.pdfenterobius vermicularisenterobiasisnested pcrcox1 gene |
| spellingShingle | Eleonora Kaneva Nina Tsvetkova Desislava Velcheva Rumen Harizanov lskren Kaftandjiev Raina Borisova Mihaela Videnova Aleksandra lvanova Ivailo Alexiev Reneta Dimitrova Maria Pavlova DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS Journal of IMAB enterobius vermicularis enterobiasis nested pcr cox1 gene |
| title | DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS |
| title_full | DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS |
| title_fullStr | DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS |
| title_full_unstemmed | DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS |
| title_short | DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS |
| title_sort | development and application of a method for genetic detection of enterobius vermicularis in samples of patients with enterobiаsis |
| topic | enterobius vermicularis enterobiasis nested pcr cox1 gene |
| url | https://www.journal-imab-bg.org/issues-2024/issue1/2024vol30-issue1-5295-5300.pdf |
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