Regulation of soybean drought response by mepiquat chloride pretreatment
IntroductionSoybean is the world’s most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) can potentially alleviate the damage caused by drought stress in plants; however, the mechanism of MC regulation of soybean dro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1149114/full |
| _version_ | 1827950402502393856 |
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| author | Xiyue Wang Xinyu Zhou Zhipeng Qu Chao Yan Chunmei Ma Jun Liu Shoukun Dong |
| author_facet | Xiyue Wang Xinyu Zhou Zhipeng Qu Chao Yan Chunmei Ma Jun Liu Shoukun Dong |
| author_sort | Xiyue Wang |
| collection | DOAJ |
| description | IntroductionSoybean is the world’s most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) can potentially alleviate the damage caused by drought stress in plants; however, the mechanism of MC regulation of soybean drought response has not been studied.MethodsThis study investigated the mechanism of soybean drought response regulation by mepiquat chloride in two varieties of soybean, sensitive Heinong 65 (HN65) and drought-tolerant Heinong44 (HN44), under three treatment scenarios, normal, drought stress, and drought stress + MC conditions.Results and discussionMC promoted dry matter accumulation under drought stress, reduced plant height, decreased antioxidant enzyme activity, and significantly decreased malondialdehyde content. The light capture processes, photosystems I and II, were inhibited; however, accumulation and upregulation of several amino acids and flavonoids by MC was observed. Multi-omics joint analysis indicated 2-oxocarboxylic acid metabolism and isoflavone biosynthetic pathways to be the core pathways by which MC regulated soybean drought response. Candidate genes such as LOC100816177, SOMT-2, LOC100784120, LOC100797504, LOC100794610, and LOC100819853 were identified to be crucial for the drought resistance of soybeans. Finally, a model was constructed to systematically describe the regulatory mechanism of MC application in soybean under drought stress. This study fills the research gap of MC in the field of soybean resistance. |
| first_indexed | 2024-04-09T13:25:30Z |
| format | Article |
| id | doaj.art-9adc6bc564c54a99b64e76bd44897856 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:25:30Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9adc6bc564c54a99b64e76bd448978562023-05-10T13:05:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11491141149114Regulation of soybean drought response by mepiquat chloride pretreatmentXiyue Wang0Xinyu Zhou1Zhipeng Qu2Chao Yan3Chunmei Ma4Jun Liu5Shoukun Dong6College of Agriculture, Northeast Agricultural University, Harbin, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaLab of Functional Genomics and Bioinformatics, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaIntroductionSoybean is the world’s most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) can potentially alleviate the damage caused by drought stress in plants; however, the mechanism of MC regulation of soybean drought response has not been studied.MethodsThis study investigated the mechanism of soybean drought response regulation by mepiquat chloride in two varieties of soybean, sensitive Heinong 65 (HN65) and drought-tolerant Heinong44 (HN44), under three treatment scenarios, normal, drought stress, and drought stress + MC conditions.Results and discussionMC promoted dry matter accumulation under drought stress, reduced plant height, decreased antioxidant enzyme activity, and significantly decreased malondialdehyde content. The light capture processes, photosystems I and II, were inhibited; however, accumulation and upregulation of several amino acids and flavonoids by MC was observed. Multi-omics joint analysis indicated 2-oxocarboxylic acid metabolism and isoflavone biosynthetic pathways to be the core pathways by which MC regulated soybean drought response. Candidate genes such as LOC100816177, SOMT-2, LOC100784120, LOC100797504, LOC100794610, and LOC100819853 were identified to be crucial for the drought resistance of soybeans. Finally, a model was constructed to systematically describe the regulatory mechanism of MC application in soybean under drought stress. This study fills the research gap of MC in the field of soybean resistance.https://www.frontiersin.org/articles/10.3389/fpls.2023.1149114/fulldrought stressmepiquat chloridesoybeanmolecular mechanismphysiologygrowth characteristic |
| spellingShingle | Xiyue Wang Xinyu Zhou Zhipeng Qu Chao Yan Chunmei Ma Jun Liu Shoukun Dong Regulation of soybean drought response by mepiquat chloride pretreatment Frontiers in Plant Science drought stress mepiquat chloride soybean molecular mechanism physiology growth characteristic |
| title | Regulation of soybean drought response by mepiquat chloride pretreatment |
| title_full | Regulation of soybean drought response by mepiquat chloride pretreatment |
| title_fullStr | Regulation of soybean drought response by mepiquat chloride pretreatment |
| title_full_unstemmed | Regulation of soybean drought response by mepiquat chloride pretreatment |
| title_short | Regulation of soybean drought response by mepiquat chloride pretreatment |
| title_sort | regulation of soybean drought response by mepiquat chloride pretreatment |
| topic | drought stress mepiquat chloride soybean molecular mechanism physiology growth characteristic |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1149114/full |
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