Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations]
Apolipoprotein E is a secreted protein involved in mediating lipid distribution and metabolism among cells of specific tissues. The dysregulation of Apolipoprotein E can disturb cholesterol homeostasis, resulting in several diseases, including cardiovascular disease and Alzheimer’s disease. The ther...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2023-09-01
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| Series: | F1000Research |
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| Online Access: | https://f1000research.com/articles/12-810/v3 |
| _version_ | 1797373083619688448 |
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| author | Carl Laflamme Kathleen Southern Riham Ayoubi |
| author_facet | Carl Laflamme Kathleen Southern Riham Ayoubi |
| author_sort | Carl Laflamme |
| collection | DOAJ |
| description | Apolipoprotein E is a secreted protein involved in mediating lipid distribution and metabolism among cells of specific tissues. The dysregulation of Apolipoprotein E can disturb cholesterol homeostasis, resulting in several diseases, including cardiovascular disease and Alzheimer’s disease. The therapeutic potential of Apolipoprotein E against these diseases demonstrates the importance of providing high-quality antibodies for this protein to the scientific community. In this study, we characterized fourteen Apolipoprotein E commercial antibodies for Western Blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs. |
| first_indexed | 2024-03-08T18:44:11Z |
| format | Article |
| id | doaj.art-9ae3b077012d49299377ad408b31fd21 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-03-08T18:44:11Z |
| publishDate | 2023-09-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-9ae3b077012d49299377ad408b31fd212023-12-29T01:00:03ZengF1000 Research LtdF1000Research2046-14022023-09-0112156691Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations]Carl Laflamme0https://orcid.org/0000-0001-5906-025XKathleen Southern1https://orcid.org/0000-0002-4125-3608Riham Ayoubi2Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaApolipoprotein E is a secreted protein involved in mediating lipid distribution and metabolism among cells of specific tissues. The dysregulation of Apolipoprotein E can disturb cholesterol homeostasis, resulting in several diseases, including cardiovascular disease and Alzheimer’s disease. The therapeutic potential of Apolipoprotein E against these diseases demonstrates the importance of providing high-quality antibodies for this protein to the scientific community. In this study, we characterized fourteen Apolipoprotein E commercial antibodies for Western Blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.https://f1000research.com/articles/12-810/v3Uniprot ID P02649 APOE Apolipoprotein E antibody characterization antibody validation Western Bloteng |
| spellingShingle | Carl Laflamme Kathleen Southern Riham Ayoubi Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] F1000Research Uniprot ID P02649 APOE Apolipoprotein E antibody characterization antibody validation Western Blot eng |
| title | Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] |
| title_full | Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] |
| title_fullStr | Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] |
| title_full_unstemmed | Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] |
| title_short | Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations] |
| title_sort | identification of high performing antibodies for apolipoprotein e for use in western blot and immunoprecipitation version 3 peer review 1 approved 2 approved with reservations |
| topic | Uniprot ID P02649 APOE Apolipoprotein E antibody characterization antibody validation Western Blot eng |
| url | https://f1000research.com/articles/12-810/v3 |
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