Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study
Introduction: Smear layer removal from the root canal wall involves the use of 17% EDTA and 5.25% sodium hypochlorite, which thereby improves the adhesion of obturating materials to root dentin. But these chemical irrigants have shown to decrease micro hardness, increase roughness, cause erosion...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-08-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8272/20819_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1818923211891408896 |
|---|---|
| author | Bhavana Gandhi Swetha Bollineni Ravi Kumar Janga Disha Saraswati Mandava Ramesh Babu |
| author_facet | Bhavana Gandhi Swetha Bollineni Ravi Kumar Janga Disha Saraswati Mandava Ramesh Babu |
| author_sort | Bhavana Gandhi |
| collection | DOAJ |
| description | Introduction: Smear layer removal from the root canal wall
involves the use of 17% EDTA and 5.25% sodium hypochlorite,
which thereby improves the adhesion of obturating materials to
root dentin. But these chemical irrigants have shown to decrease
micro hardness, increase roughness, cause erosion and reduce
the root dentin fracture toughness. To combat these adverse
effects, studies can be focussed on the remineralisation of the
erosive root dentin and this novel idea has been utilized in the
present study.
Aim: To evaluate the micro hardness of erosive root dentin
when Casein Phosphopeptide–Amorphous Calcium Phosphate
(CPP-ACP) was used as a final irrigant and its influence on resin
sealer bonding tested by push-out bond strength method.
Materials and Methods: Sixty extracted maxillary incisors were
divided into three groups based on the final irrigation protocol.
Group 1-normal saline, Group 2-17% EDTA (Ethylene Diamine
Tetraacetic Acid) + 5.25% NaOCl (Sodium Hypochlorite),
Group 3 - 17% EDTA + 5.25% NaOCl + CPP-ACP; each group
was divided into two subgroups. Half the specimens of each
group were evaluated for Vicker’s micro hardness test after
the treatment. In continuation with the above methodology the
remaining specimens were tested for push-out bond strength
after obturation of the specimens with self etch adhesive resin
sealer and conventional 6% gutta percha cones.
Results: Micro hardness was statistically analysed using
Kruskal Wallis test and push-out bond strength was evaluated
using Mann Whitney test and paired t-test. CPP-ACP treated
group showed increased micro hardness (p<0.05). There was
no statistically significant difference between the push-out
bond strength values between group EDTA + NaOCl group and
EDTA + NaOCl + CPP-ACP group.
Conclusion: Within the limitations of this study it can be
concluded that, CPP-ACP improved the micro hardness
of erosive root dentin and is not affecting its bond strength.
Therefore, CPP-ACP may be used before bonding procedures
for promoting remineralization of root dentin. |
| first_indexed | 2024-12-20T02:05:50Z |
| format | Article |
| id | doaj.art-9ae7c40e09de4642a3a38ffe221db20d |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-20T02:05:50Z |
| publishDate | 2016-08-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-9ae7c40e09de4642a3a38ffe221db20d2022-12-21T19:57:12ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-08-01108ZC53ZC5610.7860/JCDR/2016/20819.8272Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro StudyBhavana Gandhi0Swetha Bollineni1Ravi Kumar Janga2Disha Saraswati3Mandava Ramesh Babu4Assistant Professor, Department of Conservative Dentistry and Endodontics, Drs. Sudha and Nageswara Rao Institute of Dental Sciences, Gannavaram, Andhra Pradesh, India.Assistant Professor, Department of Conservative Dentistry and Endodontics, Drs. Sudha and Nageswara Rao Institute of Dental Sciences, Gannavaram, Andhra Pradesh, India.Associate Professor, Department of Conservative Dentistry and Endodontics, Drs. Sudha and Nageswara Rao Institute of Dental Sciences, Gannavaram, Andhra Pradesh, India.Professor, Department of Conservative Dentistry and Endodontics, Drs. Sudha and Nageswara Rao Institute of Dental Sciences, Gannavaram, Andhra Pradesh, India.Professor, Department of Conservative Dentistry and Endodontics, Drs. Sudha and Nageswara Rao Institute of Dental Sciences, Gannavaram, Andhra Pradesh, India.Introduction: Smear layer removal from the root canal wall involves the use of 17% EDTA and 5.25% sodium hypochlorite, which thereby improves the adhesion of obturating materials to root dentin. But these chemical irrigants have shown to decrease micro hardness, increase roughness, cause erosion and reduce the root dentin fracture toughness. To combat these adverse effects, studies can be focussed on the remineralisation of the erosive root dentin and this novel idea has been utilized in the present study. Aim: To evaluate the micro hardness of erosive root dentin when Casein Phosphopeptide–Amorphous Calcium Phosphate (CPP-ACP) was used as a final irrigant and its influence on resin sealer bonding tested by push-out bond strength method. Materials and Methods: Sixty extracted maxillary incisors were divided into three groups based on the final irrigation protocol. Group 1-normal saline, Group 2-17% EDTA (Ethylene Diamine Tetraacetic Acid) + 5.25% NaOCl (Sodium Hypochlorite), Group 3 - 17% EDTA + 5.25% NaOCl + CPP-ACP; each group was divided into two subgroups. Half the specimens of each group were evaluated for Vicker’s micro hardness test after the treatment. In continuation with the above methodology the remaining specimens were tested for push-out bond strength after obturation of the specimens with self etch adhesive resin sealer and conventional 6% gutta percha cones. Results: Micro hardness was statistically analysed using Kruskal Wallis test and push-out bond strength was evaluated using Mann Whitney test and paired t-test. CPP-ACP treated group showed increased micro hardness (p<0.05). There was no statistically significant difference between the push-out bond strength values between group EDTA + NaOCl group and EDTA + NaOCl + CPP-ACP group. Conclusion: Within the limitations of this study it can be concluded that, CPP-ACP improved the micro hardness of erosive root dentin and is not affecting its bond strength. Therefore, CPP-ACP may be used before bonding procedures for promoting remineralization of root dentin.https://jcdr.net/articles/PDF/8272/20819_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdfdentinethylenediaminetetraacetic acidresin cementsodium hypochlorite |
| spellingShingle | Bhavana Gandhi Swetha Bollineni Ravi Kumar Janga Disha Saraswati Mandava Ramesh Babu Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study Journal of Clinical and Diagnostic Research dentin ethylenediaminetetraacetic acid resin cement sodium hypochlorite |
| title | Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study |
| title_full | Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study |
| title_fullStr | Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study |
| title_full_unstemmed | Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study |
| title_short | Evaluating the Effect of CPP-ACP as a Final Irrigant in Improving the Micro-Hardness of Erosive Root Dentin and its Influence on the Bond Strength of Self Etch Resin Sealer – An In-vitro Study |
| title_sort | evaluating the effect of cpp acp as a final irrigant in improving the micro hardness of erosive root dentin and its influence on the bond strength of self etch resin sealer an in vitro study |
| topic | dentin ethylenediaminetetraacetic acid resin cement sodium hypochlorite |
| url | https://jcdr.net/articles/PDF/8272/20819_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
| work_keys_str_mv | AT bhavanagandhi evaluatingtheeffectofcppacpasafinalirrigantinimprovingthemicrohardnessoferosiverootdentinanditsinfluenceonthebondstrengthofselfetchresinsealeraninvitrostudy AT swethabollineni evaluatingtheeffectofcppacpasafinalirrigantinimprovingthemicrohardnessoferosiverootdentinanditsinfluenceonthebondstrengthofselfetchresinsealeraninvitrostudy AT ravikumarjanga evaluatingtheeffectofcppacpasafinalirrigantinimprovingthemicrohardnessoferosiverootdentinanditsinfluenceonthebondstrengthofselfetchresinsealeraninvitrostudy AT dishasaraswati evaluatingtheeffectofcppacpasafinalirrigantinimprovingthemicrohardnessoferosiverootdentinanditsinfluenceonthebondstrengthofselfetchresinsealeraninvitrostudy AT mandavarameshbabu evaluatingtheeffectofcppacpasafinalirrigantinimprovingthemicrohardnessoferosiverootdentinanditsinfluenceonthebondstrengthofselfetchresinsealeraninvitrostudy |