Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells
Objective: This study was performed to determine whether melatonin atphysiological concentrations (0.01-10nM) could affect the proliferation andosteogenic differentiation of Rat ADSCs in vitro.Materials and Methods: ADSCs were isolated from the fat of adult rats. Aftercell expansion in culture media...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2008-01-01
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| Series: | Cell Journal |
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| Online Access: | http://celljournal.org/library/upload/article/Zaminy.pdf |
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| author | Arash Zaminy, Iraj Ragerdi Kashani Mohammad Barbarestani Azim Hedayatpour Reza Mahmoudi Safoura Vardasbi Mohammad Ali Shokrgozar |
| author_facet | Arash Zaminy, Iraj Ragerdi Kashani Mohammad Barbarestani Azim Hedayatpour Reza Mahmoudi Safoura Vardasbi Mohammad Ali Shokrgozar |
| author_sort | Arash Zaminy, |
| collection | DOAJ |
| description | Objective: This study was performed to determine whether melatonin atphysiological concentrations (0.01-10nM) could affect the proliferation andosteogenic differentiation of Rat ADSCs in vitro.Materials and Methods: ADSCs were isolated from the fat of adult rats. Aftercell expansion in culture media and through three passages, osteogenesiswas induced on a monolayer culture with osteogenic medium with or withoutmelatonin at physiological concentrations (0.01-10nM). After 4 weeks cultureswere examined for mineralization by Alizarin Red S and von Kossa staining andfor alkaline phosphatase (ALP) activity by ALP kit. Cell viability and apoptosiswere also assayed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-ulfophenyl)-2H-tetrazolium assay and flowcytometry, respectively. Allassays were performed in triplicate.Results: The results indicated that at physiological concentrations, melatoninsuppressed proliferation and differentiation of ADSCs. These data indicate thatADSCs exposed to melatonin, had a lower ALP activity in contrast to the cellsexposed to the osteogenic medium alone. Similarly, the mineral deposition(calcium level) also decreased. The flow cytometry proved that the cell growthdecreased and the apoptotic cells increased.Conclusion: These results suggest that physiological concentration ofmelatonin has a negative effect on ADSCs osteogenesis. |
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| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-12T06:18:20Z |
| publishDate | 2008-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-9ae8e257e3d7482ab25ec264b84ee2842022-12-22T03:44:23ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142008-01-011012532Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem CellsArash Zaminy,Iraj Ragerdi KashaniMohammad BarbarestaniAzim HedayatpourReza MahmoudiSafoura VardasbiMohammad Ali ShokrgozarObjective: This study was performed to determine whether melatonin atphysiological concentrations (0.01-10nM) could affect the proliferation andosteogenic differentiation of Rat ADSCs in vitro.Materials and Methods: ADSCs were isolated from the fat of adult rats. Aftercell expansion in culture media and through three passages, osteogenesiswas induced on a monolayer culture with osteogenic medium with or withoutmelatonin at physiological concentrations (0.01-10nM). After 4 weeks cultureswere examined for mineralization by Alizarin Red S and von Kossa staining andfor alkaline phosphatase (ALP) activity by ALP kit. Cell viability and apoptosiswere also assayed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-ulfophenyl)-2H-tetrazolium assay and flowcytometry, respectively. Allassays were performed in triplicate.Results: The results indicated that at physiological concentrations, melatoninsuppressed proliferation and differentiation of ADSCs. These data indicate thatADSCs exposed to melatonin, had a lower ALP activity in contrast to the cellsexposed to the osteogenic medium alone. Similarly, the mineral deposition(calcium level) also decreased. The flow cytometry proved that the cell growthdecreased and the apoptotic cells increased.Conclusion: These results suggest that physiological concentration ofmelatonin has a negative effect on ADSCs osteogenesis.http://celljournal.org/library/upload/article/Zaminy.pdfAdipose TissueStem CellsMelatoninOsteogenic Differentiation |
| spellingShingle | Arash Zaminy, Iraj Ragerdi Kashani Mohammad Barbarestani Azim Hedayatpour Reza Mahmoudi Safoura Vardasbi Mohammad Ali Shokrgozar Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells Cell Journal Adipose Tissue Stem Cells Melatonin Osteogenic Differentiation |
| title | Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells |
| title_full | Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells |
| title_fullStr | Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells |
| title_full_unstemmed | Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells |
| title_short | Melatonin Influences the Proliferative and Differentiative Activity of Rat Adipose-Derived Stem Cells |
| title_sort | melatonin influences the proliferative and differentiative activity of rat adipose derived stem cells |
| topic | Adipose Tissue Stem Cells Melatonin Osteogenic Differentiation |
| url | http://celljournal.org/library/upload/article/Zaminy.pdf |
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