The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study
Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2021-03-01
|
| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v19i3.8575 |
| _version_ | 1831539918830043136 |
|---|---|
| author | Samira Khani Maasoume Abdollahi Azam Khalaj Hamid Heidari Somaye Zohali |
| author_facet | Samira Khani Maasoume Abdollahi Azam Khalaj Hamid Heidari Somaye Zohali |
| author_sort | Samira Khani |
| collection | DOAJ |
| description | Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women.
Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats.
Materials and Methods: This experimental study was carried out on 36 Wistar female rats (3 wk, 60
±
10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study.
Results: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p
≤
0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p
≤
0.001).
Conclusion: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective. |
| first_indexed | 2024-12-16T23:49:33Z |
| format | Article |
| id | doaj.art-9ae962442a1c4dafa9d1492875547b8e |
| institution | Directory Open Access Journal |
| issn | 2476-3772 |
| language | English |
| last_indexed | 2024-12-16T23:49:33Z |
| publishDate | 2021-03-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-9ae962442a1c4dafa9d1492875547b8e2022-12-21T22:11:23ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-37722021-03-011927128210.18502/ijrm.v19i3.8575ijrm.v19i3.8575The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental studySamira Khani0Maasoume Abdollahi1Azam Khalaj2Hamid Heidari3Somaye Zohali4 Student Research Committee, Qom University of Medical Sciences, Qom, Iran. Student Research Committee, Qom University of Medical Sciences, Qom, Iran. Student Research Committee, Qom University of Medical Sciences, Qom, Iran. Student Research Committee, Qom University of Medical Sciences, Qom, Iran. Student Research Committee, Qom University of Medical Sciences, Qom, Iran.Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats. Materials and Methods: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 ± 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study. Results: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p ≤ 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p ≤ 0.001). Conclusion: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective.https://doi.org/10.18502/ijrm.v19i3.8575nigella sativa seed, oxidative stress, insulin resistance, polycystic ovary syndrome, rat. |
| spellingShingle | Samira Khani Maasoume Abdollahi Azam Khalaj Hamid Heidari Somaye Zohali The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study International Journal of Reproductive BioMedicine nigella sativa seed, oxidative stress, insulin resistance, polycystic ovary syndrome, rat. |
| title | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
| title_full | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
| title_fullStr | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
| title_full_unstemmed | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
| title_short | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
| title_sort | effect of hydroalcoholic extract of nigella sativa seed on dehydroepiandrosterone induced polycystic ovarian syndrome in rats an experimental study |
| topic | nigella sativa seed, oxidative stress, insulin resistance, polycystic ovary syndrome, rat. |
| url | https://doi.org/10.18502/ijrm.v19i3.8575 |
| work_keys_str_mv | AT samirakhani theeffectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT maasoumeabdollahi theeffectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT azamkhalaj theeffectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT hamidheidari theeffectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT somayezohali theeffectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT samirakhani effectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT maasoumeabdollahi effectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT azamkhalaj effectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT hamidheidari effectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy AT somayezohali effectofhydroalcoholicextractofnigellasativaseedondehydroepiandrosteroneinducedpolycysticovariansyndromeinratsanexperimentalstudy |