Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts

Abstract Background: Prenatal stress may increase risk of developing cardiovascular disorders in adulthood. The cardiotoxic effects of catecholamines are mediated via prolonged adrenergic receptor stimulation and increased oxidative stress upon their degradation by monoamine oxidase A (MAO-A). Obj...

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Main Authors: Tanja Jevjdovic, Tamara Dakic, Sonja Kopanja, Iva Lakic, Predrag Vujovic, Nebojsa Jasnic, Jelena Djordjevic
Format: Article
Language:English
Published: Sociedade Brasileira de Cardiologia (SBC) 2018-12-01
Series:Arquivos Brasileiros de Cardiologia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018005018102&lng=en&tlng=en
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author Tanja Jevjdovic
Tamara Dakic
Sonja Kopanja
Iva Lakic
Predrag Vujovic
Nebojsa Jasnic
Jelena Djordjevic
author_facet Tanja Jevjdovic
Tamara Dakic
Sonja Kopanja
Iva Lakic
Predrag Vujovic
Nebojsa Jasnic
Jelena Djordjevic
author_sort Tanja Jevjdovic
collection DOAJ
description Abstract Background: Prenatal stress may increase risk of developing cardiovascular disorders in adulthood. The cardiotoxic effects of catecholamines are mediated via prolonged adrenergic receptor stimulation and increased oxidative stress upon their degradation by monoamine oxidase A (MAO-A). Objectives: We investigated long-term effects of prenatal stress on β (1, 2, 3) adrenergic receptors and MAO-A gene expression in the hearts of adult rat offspring. Methods: Pregnant rats were exposed to unpredictable mild stress during the third week of gestation. RNA was isolated from left ventricular apex and base of adult offspring. Quantitative PCR was used to measure gene expression in collected ventricular tissue samples. The level of significance was set to p < 0.05. Results: β3 adrenergic receptor mRNA was undetectable in rat left ventricle. β1 adrenergic receptor was the predominantly expressed subtype at the apical and basal left ventricular myocardium in the control females. Male offspring from unstressed mothers displayed higher apical cardiac β1 than β2 adrenergic receptor mRNA levels. However, β1 and β2 adrenergic receptor mRNAs were similarly expressed at the ventricular basal myocardium in males. Unlike males, prenatally stressed females exhibited decreased β1 adrenergic receptor mRNA expression at the apical myocardium. Prenatal stress did not affect cardiac MAO-A gene expression. Conclusions: Collectively, our results show that prenatal stress may have exerted region- and sex-specific β1 and β2 adrenergic receptor expression patterns within the left ventricle.
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spelling doaj.art-9af04ff4f70c44368a19772c3f4ac79d2022-12-22T02:02:08ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia1678-41702018-12-01010.5935/abc.20190001S0066-782X2018005018102Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat HeartsTanja JevjdovicTamara DakicSonja KopanjaIva LakicPredrag VujovicNebojsa JasnicJelena DjordjevicAbstract Background: Prenatal stress may increase risk of developing cardiovascular disorders in adulthood. The cardiotoxic effects of catecholamines are mediated via prolonged adrenergic receptor stimulation and increased oxidative stress upon their degradation by monoamine oxidase A (MAO-A). Objectives: We investigated long-term effects of prenatal stress on β (1, 2, 3) adrenergic receptors and MAO-A gene expression in the hearts of adult rat offspring. Methods: Pregnant rats were exposed to unpredictable mild stress during the third week of gestation. RNA was isolated from left ventricular apex and base of adult offspring. Quantitative PCR was used to measure gene expression in collected ventricular tissue samples. The level of significance was set to p < 0.05. Results: β3 adrenergic receptor mRNA was undetectable in rat left ventricle. β1 adrenergic receptor was the predominantly expressed subtype at the apical and basal left ventricular myocardium in the control females. Male offspring from unstressed mothers displayed higher apical cardiac β1 than β2 adrenergic receptor mRNA levels. However, β1 and β2 adrenergic receptor mRNAs were similarly expressed at the ventricular basal myocardium in males. Unlike males, prenatally stressed females exhibited decreased β1 adrenergic receptor mRNA expression at the apical myocardium. Prenatal stress did not affect cardiac MAO-A gene expression. Conclusions: Collectively, our results show that prenatal stress may have exerted region- and sex-specific β1 and β2 adrenergic receptor expression patterns within the left ventricle.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018005018102&lng=en&tlng=enPregnancyStress, PhysiologicalOxidative StressHeartCatecholaminesRatsSexFemaleCardiotoxicityAdrenergic beta1 beta2 Receptor Antagonists
spellingShingle Tanja Jevjdovic
Tamara Dakic
Sonja Kopanja
Iva Lakic
Predrag Vujovic
Nebojsa Jasnic
Jelena Djordjevic
Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
Arquivos Brasileiros de Cardiologia
Pregnancy
Stress, Physiological
Oxidative Stress
Heart
Catecholamines
Rats
Sex
Female
Cardiotoxicity
Adrenergic beta1 beta2 Receptor Antagonists
title Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
title_full Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
title_fullStr Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
title_full_unstemmed Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
title_short Sex-Related Effects of Prenatal Stress on Region-Specific Expression of Monoamine Oxidase A and β Adrenergic Receptors in Rat Hearts
title_sort sex related effects of prenatal stress on region specific expression of monoamine oxidase a and β adrenergic receptors in rat hearts
topic Pregnancy
Stress, Physiological
Oxidative Stress
Heart
Catecholamines
Rats
Sex
Female
Cardiotoxicity
Adrenergic beta1 beta2 Receptor Antagonists
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018005018102&lng=en&tlng=en
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