Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds
Lectins are proteins that have the ability to bind specifically and reversibly to carbohydrates and glycoconjugates, without altering the structure of the glycosyl ligand. They are found in organisms such as viruses, plants and humans, and they have been shown to possess important biological activit...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/3/3277/ |
| _version_ | 1819030323228311552 |
|---|---|
| author | Tatiane Santi-Gadelha Benildo Sousa Cavada Plinio Delatorre Hilzeth de Luna Freire Pessôa Carlos Alberto de Almeida Gadelha Vinícius de Morais Gomes João Batista Cajazeiras Kyria Santiago do Nascimento Rodrigo Rodrigues e Lacerda Mário Rogério Lima Mota Joana Filomena Magalhães Leite Pedro Henrique de Souza Ferreira Bringel Ana Maria Sampaio Assreuy |
| author_facet | Tatiane Santi-Gadelha Benildo Sousa Cavada Plinio Delatorre Hilzeth de Luna Freire Pessôa Carlos Alberto de Almeida Gadelha Vinícius de Morais Gomes João Batista Cajazeiras Kyria Santiago do Nascimento Rodrigo Rodrigues e Lacerda Mário Rogério Lima Mota Joana Filomena Magalhães Leite Pedro Henrique de Souza Ferreira Bringel Ana Maria Sampaio Assreuy |
| author_sort | Tatiane Santi-Gadelha |
| collection | DOAJ |
| description | Lectins are proteins that have the ability to bind specifically and reversibly to carbohydrates and glycoconjugates, without altering the structure of the glycosyl ligand. They are found in organisms such as viruses, plants and humans, and they have been shown to possess important biological activities. The objective of this study was to purify and characterize lectins in the seeds of Clitoria fairchildiana, as well as to verify their biological activities. The results indicated the presence of a lectin (CFAL) in the glutelin acid protein fraction, which agglutinated native rabbit erythrocytes. CFAL was purified by column chromatography ion-exchange, DEAE-Sephacel, which was obtained from a peak of protein retained in the matrix by applying 0.5 M NaCl using the step-wise method. Electrophoretic analysis of this lectin in SDS-PAGE indicated a two band pattern protein molecular mass of approximately 100 and 116 kDa. CFAL proved to be unspecific to all carbohydrates/glycoconjugates in common use for the sugar inhibition test. This lectin showed no significant cytotoxicity to human red blood cells. It was observed that CFAL has anti-inflammatory activity in the paw edema induced by carrageenan model, in which a 64% diminution in edema was observed. Antinociceptive effects were observed for CFAL in the abdominal writhing test (induced by acetic acid), in which increasing doses of the lectin caused reduction in the number of contortions by up to 72%. It was concluded that the purified and characterized lectin from the seeds of Clitoria fairchildiana has anti-inflammatory and antinociceptive activity, and is not cytotoxic to human erythrocytes. |
| first_indexed | 2024-12-21T06:28:20Z |
| format | Article |
| id | doaj.art-9af182b4f890425d8be692011bdcccd3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T06:28:20Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9af182b4f890425d8be692011bdcccd32022-12-21T19:13:04ZengMDPI AGMolecules1420-30492012-03-011733277329010.3390/molecules17033277Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard SeedsTatiane Santi-GadelhaBenildo Sousa CavadaPlinio DelatorreHilzeth de Luna Freire PessôaCarlos Alberto de Almeida GadelhaVinícius de Morais GomesJoão Batista CajazeirasKyria Santiago do NascimentoRodrigo Rodrigues e LacerdaMário Rogério Lima MotaJoana Filomena Magalhães LeitePedro Henrique de Souza Ferreira BringelAna Maria Sampaio AssreuyLectins are proteins that have the ability to bind specifically and reversibly to carbohydrates and glycoconjugates, without altering the structure of the glycosyl ligand. They are found in organisms such as viruses, plants and humans, and they have been shown to possess important biological activities. The objective of this study was to purify and characterize lectins in the seeds of Clitoria fairchildiana, as well as to verify their biological activities. The results indicated the presence of a lectin (CFAL) in the glutelin acid protein fraction, which agglutinated native rabbit erythrocytes. CFAL was purified by column chromatography ion-exchange, DEAE-Sephacel, which was obtained from a peak of protein retained in the matrix by applying 0.5 M NaCl using the step-wise method. Electrophoretic analysis of this lectin in SDS-PAGE indicated a two band pattern protein molecular mass of approximately 100 and 116 kDa. CFAL proved to be unspecific to all carbohydrates/glycoconjugates in common use for the sugar inhibition test. This lectin showed no significant cytotoxicity to human red blood cells. It was observed that CFAL has anti-inflammatory activity in the paw edema induced by carrageenan model, in which a 64% diminution in edema was observed. Antinociceptive effects were observed for CFAL in the abdominal writhing test (induced by acetic acid), in which increasing doses of the lectin caused reduction in the number of contortions by up to 72%. It was concluded that the purified and characterized lectin from the seeds of Clitoria fairchildiana has anti-inflammatory and antinociceptive activity, and is not cytotoxic to human erythrocytes.http://www.mdpi.com/1420-3049/17/3/3277/Clitoriapurificationglycoproteintoxicitybiological activity |
| spellingShingle | Tatiane Santi-Gadelha Benildo Sousa Cavada Plinio Delatorre Hilzeth de Luna Freire Pessôa Carlos Alberto de Almeida Gadelha Vinícius de Morais Gomes João Batista Cajazeiras Kyria Santiago do Nascimento Rodrigo Rodrigues e Lacerda Mário Rogério Lima Mota Joana Filomena Magalhães Leite Pedro Henrique de Souza Ferreira Bringel Ana Maria Sampaio Assreuy Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds Molecules Clitoria purification glycoprotein toxicity biological activity |
| title | Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds |
| title_full | Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds |
| title_fullStr | Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds |
| title_full_unstemmed | Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds |
| title_short | Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds |
| title_sort | antinociceptive and anti inflammatory effects of a lectin like substance from clitoria fairchildiana r howard seeds |
| topic | Clitoria purification glycoprotein toxicity biological activity |
| url | http://www.mdpi.com/1420-3049/17/3/3277/ |
| work_keys_str_mv | AT tatianesantigadelha antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT benildosousacavada antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT pliniodelatorre antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT hilzethdelunafreirepessoa antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT carlosalbertodealmeidagadelha antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT viniciusdemoraisgomes antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT joaobatistacajazeiras antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT kyriasantiagodonascimento antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT rodrigorodrigueselacerda antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT mariorogeriolimamota antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT joanafilomenamagalhaesleite antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT pedrohenriquedesouzaferreirabringel antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds AT anamariasampaioassreuy antinociceptiveandantiinflammatoryeffectsofalectinlikesubstancefromclitoriafairchildianarhowardseeds |