Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product
Lyophilization is one of the most used methods for bacterial preservation. In this process, the cryoprotectant not only largely decreases cellular damage but also plays an important part in the conservation of viability during freeze-drying. This study investigated using cryoprotectant and a mixture...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/19/3113 |
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| author | Zin Myo Swe Thapakorn Chumphon Marutpong Panya Kanjana Pangjit Saran Promsai |
| author_facet | Zin Myo Swe Thapakorn Chumphon Marutpong Panya Kanjana Pangjit Saran Promsai |
| author_sort | Zin Myo Swe |
| collection | DOAJ |
| description | Lyophilization is one of the most used methods for bacterial preservation. In this process, the cryoprotectant not only largely decreases cellular damage but also plays an important part in the conservation of viability during freeze-drying. This study investigated using cryoprotectant and a mixture of the cryoprotectant to maintain probiotic activity. Seven probiotic strains were considered: (<i>Limosilactobacillus reuteri</i> KUKPS6103; <i>Lacticaseibacillus rhamnosus</i> KUKPS6007; <i>Lacticaseibacillus paracasei</i> KUKPS6201; <i>Lactobacillus acidophilus</i> KUKPS6107; <i>Ligilactobacillus salivarius</i> KUKPS6202; <i>Bacillus coagulans</i> KPSTF02; <i>Saccharomyces cerevisiae</i> subsp. <i>boulardii</i> KUKPS6005) for the production of a multi-strain probiotic and the complex medium for the lyophilized synbiotic production. Cholesterol removal, antioxidant activity, biofilm formation and gamma aminobutyric acid (GABA) production of the probiotic strains were analyzed. The most biofilm formation occurred in <i>L. reuteri</i> KUKPS6103 and the least in <i>B. coagulans</i> KPSTF02. The multi-strain probiotic had the highest cholesterol removal. All the probiotic strains had GABA production that matched the standard of γ-aminobutyric acid. The lyophilized synbiotic product containing complex medium as a cryoprotectant and wall material retained a high viability of 7.53 × 10<sup>8</sup> CFU/g (8.89 log CFU/g) after 8 weeks of storage. We found that the survival rate of the multi-strain probiotic after freeze-drying was 15.37% in the presence of complex medium that was used as high performing wall material. Our findings provided a new type of wall material that is safer and more effective and, can be extensively applied in relevant food applications. |
| first_indexed | 2024-03-09T21:44:14Z |
| format | Article |
| id | doaj.art-9af21e886a3948b9acf4ab71e8da3f21 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:44:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-9af21e886a3948b9acf4ab71e8da3f212023-11-23T20:22:20ZengMDPI AGFoods2304-81582022-10-011119311310.3390/foods11193113Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic ProductZin Myo Swe0Thapakorn Chumphon1Marutpong Panya2Kanjana Pangjit3Saran Promsai4Bioproduct Science Program, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandBioproduct Science Program, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandBioproduct Science Program, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandLyophilization is one of the most used methods for bacterial preservation. In this process, the cryoprotectant not only largely decreases cellular damage but also plays an important part in the conservation of viability during freeze-drying. This study investigated using cryoprotectant and a mixture of the cryoprotectant to maintain probiotic activity. Seven probiotic strains were considered: (<i>Limosilactobacillus reuteri</i> KUKPS6103; <i>Lacticaseibacillus rhamnosus</i> KUKPS6007; <i>Lacticaseibacillus paracasei</i> KUKPS6201; <i>Lactobacillus acidophilus</i> KUKPS6107; <i>Ligilactobacillus salivarius</i> KUKPS6202; <i>Bacillus coagulans</i> KPSTF02; <i>Saccharomyces cerevisiae</i> subsp. <i>boulardii</i> KUKPS6005) for the production of a multi-strain probiotic and the complex medium for the lyophilized synbiotic production. Cholesterol removal, antioxidant activity, biofilm formation and gamma aminobutyric acid (GABA) production of the probiotic strains were analyzed. The most biofilm formation occurred in <i>L. reuteri</i> KUKPS6103 and the least in <i>B. coagulans</i> KPSTF02. The multi-strain probiotic had the highest cholesterol removal. All the probiotic strains had GABA production that matched the standard of γ-aminobutyric acid. The lyophilized synbiotic product containing complex medium as a cryoprotectant and wall material retained a high viability of 7.53 × 10<sup>8</sup> CFU/g (8.89 log CFU/g) after 8 weeks of storage. We found that the survival rate of the multi-strain probiotic after freeze-drying was 15.37% in the presence of complex medium that was used as high performing wall material. Our findings provided a new type of wall material that is safer and more effective and, can be extensively applied in relevant food applications.https://www.mdpi.com/2304-8158/11/19/3113gum arabiccryoprotectantfreeze-dryingprebioticprobioticsynbiotic |
| spellingShingle | Zin Myo Swe Thapakorn Chumphon Marutpong Panya Kanjana Pangjit Saran Promsai Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product Foods gum arabic cryoprotectant freeze-drying prebiotic probiotic synbiotic |
| title | Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product |
| title_full | Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product |
| title_fullStr | Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product |
| title_full_unstemmed | Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product |
| title_short | Evaluation of Nano-Wall Material for Production of Novel Lyophilized-Probiotic Product |
| title_sort | evaluation of nano wall material for production of novel lyophilized probiotic product |
| topic | gum arabic cryoprotectant freeze-drying prebiotic probiotic synbiotic |
| url | https://www.mdpi.com/2304-8158/11/19/3113 |
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