Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes
Expression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express <i>Tas2r108</i>, <i>Tas2r126</i>, <i>Tas2r135</i>, <i>Tas2r137</i&...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/15/8120 |
| Summary: | Expression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express <i>Tas2r108</i>, <i>Tas2r126</i>, <i>Tas2r135</i>, <i>Tas2r137</i>, and <i>Tas2r143</i>, but the function of T2Rs in adipocytes remains unclear. Here, we show that fasting and stimulation by bitter compounds both increased <i>Tas2r</i> expression in mouse white adipose tissue, and serum starvation and stimulation by bitter compounds both increased the expression of <i>Tas2r</i> genes in 3T3-L1 adipocytes, suggesting that T2Rs have functional roles in adipocytes. RNA sequencing analysis of 3T3-L1 adipocytes stimulated by epicatechin, the ligand of Tas2r126, suggested that this receptor may play a role in the differentiation of adipocytes. Overexpression of <i>Tas2r126</i> in 3T3-L1 preadipocytes decreases fat accumulation after induction of differentiation and reduces the expression of adipogenic genes. Together, these results indicate that Tas2r126 may be involved in adipocyte differentiation. |
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| ISSN: | 1661-6596 1422-0067 |