Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes
Expression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express <i>Tas2r108</i>, <i>Tas2r126</i>, <i>Tas2r135</i>, <i>Tas2r137</i&...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8120 |
| _version_ | 1797413823547703296 |
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| author | Shunsuke Kimura Ai Tsuruma Eisuke Kato |
| author_facet | Shunsuke Kimura Ai Tsuruma Eisuke Kato |
| author_sort | Shunsuke Kimura |
| collection | DOAJ |
| description | Expression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express <i>Tas2r108</i>, <i>Tas2r126</i>, <i>Tas2r135</i>, <i>Tas2r137</i>, and <i>Tas2r143</i>, but the function of T2Rs in adipocytes remains unclear. Here, we show that fasting and stimulation by bitter compounds both increased <i>Tas2r</i> expression in mouse white adipose tissue, and serum starvation and stimulation by bitter compounds both increased the expression of <i>Tas2r</i> genes in 3T3-L1 adipocytes, suggesting that T2Rs have functional roles in adipocytes. RNA sequencing analysis of 3T3-L1 adipocytes stimulated by epicatechin, the ligand of Tas2r126, suggested that this receptor may play a role in the differentiation of adipocytes. Overexpression of <i>Tas2r126</i> in 3T3-L1 preadipocytes decreases fat accumulation after induction of differentiation and reduces the expression of adipogenic genes. Together, these results indicate that Tas2r126 may be involved in adipocyte differentiation. |
| first_indexed | 2024-03-09T05:24:30Z |
| format | Article |
| id | doaj.art-9af27f92b23240ddb9381529f8455988 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:24:30Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9af27f92b23240ddb9381529f84559882023-12-03T12:38:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315812010.3390/ijms23158120Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 PreadipocytesShunsuke Kimura0Ai Tsuruma1Eisuke Kato2Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanGraduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanDivision of Fundamental AgriScience and Research, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanExpression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express <i>Tas2r108</i>, <i>Tas2r126</i>, <i>Tas2r135</i>, <i>Tas2r137</i>, and <i>Tas2r143</i>, but the function of T2Rs in adipocytes remains unclear. Here, we show that fasting and stimulation by bitter compounds both increased <i>Tas2r</i> expression in mouse white adipose tissue, and serum starvation and stimulation by bitter compounds both increased the expression of <i>Tas2r</i> genes in 3T3-L1 adipocytes, suggesting that T2Rs have functional roles in adipocytes. RNA sequencing analysis of 3T3-L1 adipocytes stimulated by epicatechin, the ligand of Tas2r126, suggested that this receptor may play a role in the differentiation of adipocytes. Overexpression of <i>Tas2r126</i> in 3T3-L1 preadipocytes decreases fat accumulation after induction of differentiation and reduces the expression of adipogenic genes. Together, these results indicate that Tas2r126 may be involved in adipocyte differentiation.https://www.mdpi.com/1422-0067/23/15/8120taste 2 receptorbitter taste receptorepicatechin3T3-L1 adipocytedifferentiationlipid accumulation |
| spellingShingle | Shunsuke Kimura Ai Tsuruma Eisuke Kato Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes International Journal of Molecular Sciences taste 2 receptor bitter taste receptor epicatechin 3T3-L1 adipocyte differentiation lipid accumulation |
| title | Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes |
| title_full | Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes |
| title_fullStr | Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes |
| title_full_unstemmed | Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes |
| title_short | Taste 2 Receptor Is Involved in Differentiation of 3T3-L1 Preadipocytes |
| title_sort | taste 2 receptor is involved in differentiation of 3t3 l1 preadipocytes |
| topic | taste 2 receptor bitter taste receptor epicatechin 3T3-L1 adipocyte differentiation lipid accumulation |
| url | https://www.mdpi.com/1422-0067/23/15/8120 |
| work_keys_str_mv | AT shunsukekimura taste2receptorisinvolvedindifferentiationof3t3l1preadipocytes AT aitsuruma taste2receptorisinvolvedindifferentiationof3t3l1preadipocytes AT eisukekato taste2receptorisinvolvedindifferentiationof3t3l1preadipocytes |