Behavior of dicentric chromosomes in budding yeast.
DNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two centromeres attached to opposite spindle pole bodies. Repair of the DSBs generates phenotypic diversity due to the range of monocentric derivative chromosomes that...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-03-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009442 |
| _version_ | 1818667053670727680 |
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| author | Diana Cook Sarah Long John Stanton Patrick Cusick Colleen Lawrimore Elaine Yeh Sarah Grant Kerry Bloom |
| author_facet | Diana Cook Sarah Long John Stanton Patrick Cusick Colleen Lawrimore Elaine Yeh Sarah Grant Kerry Bloom |
| author_sort | Diana Cook |
| collection | DOAJ |
| description | DNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two centromeres attached to opposite spindle pole bodies. Repair of the DSBs generates phenotypic diversity due to the range of monocentric derivative chromosomes that arise. To explore whether DSBs may be differentially repaired as a function of their spatial position in the chromosome, we have examined the structure of monocentric derivative chromosomes from cells containing a suite of dicentric chromosomes in which the distance between the two centromeres ranges from 6.5 kb to 57.7 kb. Two major classes of repair products, homology-based (homologous recombination (HR) and single-strand annealing (SSA)) and end-joining (non-homologous (NHEJ) and micro-homology mediated (MMEJ)) were identified. The distribution of repair products varies as a function of distance between the two centromeres. Genetic dependencies on double strand break repair (Rad52), DNA ligase (Lif1), and S phase checkpoint (Mrc1) are indicative of distinct repair pathway choices for DNA breaks in the pericentromeric chromatin versus the arms. |
| first_indexed | 2024-12-17T06:14:19Z |
| format | Article |
| id | doaj.art-9af94107f64741619738cdc9fb8c86fd |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-17T06:14:19Z |
| publishDate | 2021-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-9af94107f64741619738cdc9fb8c86fd2022-12-21T22:00:33ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-03-01173e100944210.1371/journal.pgen.1009442Behavior of dicentric chromosomes in budding yeast.Diana CookSarah LongJohn StantonPatrick CusickColleen LawrimoreElaine YehSarah GrantKerry BloomDNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two centromeres attached to opposite spindle pole bodies. Repair of the DSBs generates phenotypic diversity due to the range of monocentric derivative chromosomes that arise. To explore whether DSBs may be differentially repaired as a function of their spatial position in the chromosome, we have examined the structure of monocentric derivative chromosomes from cells containing a suite of dicentric chromosomes in which the distance between the two centromeres ranges from 6.5 kb to 57.7 kb. Two major classes of repair products, homology-based (homologous recombination (HR) and single-strand annealing (SSA)) and end-joining (non-homologous (NHEJ) and micro-homology mediated (MMEJ)) were identified. The distribution of repair products varies as a function of distance between the two centromeres. Genetic dependencies on double strand break repair (Rad52), DNA ligase (Lif1), and S phase checkpoint (Mrc1) are indicative of distinct repair pathway choices for DNA breaks in the pericentromeric chromatin versus the arms.https://doi.org/10.1371/journal.pgen.1009442 |
| spellingShingle | Diana Cook Sarah Long John Stanton Patrick Cusick Colleen Lawrimore Elaine Yeh Sarah Grant Kerry Bloom Behavior of dicentric chromosomes in budding yeast. PLoS Genetics |
| title | Behavior of dicentric chromosomes in budding yeast. |
| title_full | Behavior of dicentric chromosomes in budding yeast. |
| title_fullStr | Behavior of dicentric chromosomes in budding yeast. |
| title_full_unstemmed | Behavior of dicentric chromosomes in budding yeast. |
| title_short | Behavior of dicentric chromosomes in budding yeast. |
| title_sort | behavior of dicentric chromosomes in budding yeast |
| url | https://doi.org/10.1371/journal.pgen.1009442 |
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