Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses
The local X-ray-induced dynamics that occur in protein crystals during serial femtosecond crystallography (SFX) measurements at XFELs are not well understood. Here the authors performed a time-resolved X-ray pump X-ray probe SFX experiment, and they observe distinct structural changes in the disulfi...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-04-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-15610-4 |
| _version_ | 1819195123089539072 |
|---|---|
| author | Karol Nass Alexander Gorel Malik M. Abdullah Andrew V. Martin Marco Kloos Agostino Marinelli Andrew Aquila Thomas R. M. Barends Franz-Josef Decker R. Bruce Doak Lutz Foucar Elisabeth Hartmann Mario Hilpert Mark S. Hunter Zoltan Jurek Jason E. Koglin Alexander Kozlov Alberto A. Lutman Gabriela Nass Kovacs Christopher M. Roome Robert L. Shoeman Robin Santra Harry M. Quiney Beata Ziaja Sébastien Boutet Ilme Schlichting |
| author_facet | Karol Nass Alexander Gorel Malik M. Abdullah Andrew V. Martin Marco Kloos Agostino Marinelli Andrew Aquila Thomas R. M. Barends Franz-Josef Decker R. Bruce Doak Lutz Foucar Elisabeth Hartmann Mario Hilpert Mark S. Hunter Zoltan Jurek Jason E. Koglin Alexander Kozlov Alberto A. Lutman Gabriela Nass Kovacs Christopher M. Roome Robert L. Shoeman Robin Santra Harry M. Quiney Beata Ziaja Sébastien Boutet Ilme Schlichting |
| author_sort | Karol Nass |
| collection | DOAJ |
| description | The local X-ray-induced dynamics that occur in protein crystals during serial femtosecond crystallography (SFX) measurements at XFELs are not well understood. Here the authors performed a time-resolved X-ray pump X-ray probe SFX experiment, and they observe distinct structural changes in the disulfide bridges and peptide backbone of proteins; complementing theoretical approaches allow them to further characterize the details of the X-ray induced ionization and local structural dynamics. |
| first_indexed | 2024-12-23T02:07:45Z |
| format | Article |
| id | doaj.art-9afb811b5338411fb4f4557becfc7546 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-23T02:07:45Z |
| publishDate | 2020-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-9afb811b5338411fb4f4557becfc75462022-12-21T18:03:51ZengNature PortfolioNature Communications2041-17232020-04-011111910.1038/s41467-020-15610-4Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulsesKarol Nass0Alexander Gorel1Malik M. Abdullah2Andrew V. Martin3Marco Kloos4Agostino Marinelli5Andrew Aquila6Thomas R. M. Barends7Franz-Josef Decker8R. Bruce Doak9Lutz Foucar10Elisabeth Hartmann11Mario Hilpert12Mark S. Hunter13Zoltan Jurek14Jason E. Koglin15Alexander Kozlov16Alberto A. Lutman17Gabriela Nass Kovacs18Christopher M. Roome19Robert L. Shoeman20Robin Santra21Harry M. Quiney22Beata Ziaja23Sébastien Boutet24Ilme Schlichting25Max-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESYSchool of Science, RMIT UniversityMax-Planck-Institut für Medizinische ForschungSLAC National Accelerator LaboratorySLAC National Accelerator LaboratoryMax-Planck-Institut für Medizinische ForschungSLAC National Accelerator LaboratoryMax-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungSLAC National Accelerator LaboratoryCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESYSLAC National Accelerator LaboratoryARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of MelbourneSLAC National Accelerator LaboratoryMax-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungMax-Planck-Institut für Medizinische ForschungCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESYARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of MelbourneCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESYSLAC National Accelerator LaboratoryMax-Planck-Institut für Medizinische ForschungThe local X-ray-induced dynamics that occur in protein crystals during serial femtosecond crystallography (SFX) measurements at XFELs are not well understood. Here the authors performed a time-resolved X-ray pump X-ray probe SFX experiment, and they observe distinct structural changes in the disulfide bridges and peptide backbone of proteins; complementing theoretical approaches allow them to further characterize the details of the X-ray induced ionization and local structural dynamics.https://doi.org/10.1038/s41467-020-15610-4 |
| spellingShingle | Karol Nass Alexander Gorel Malik M. Abdullah Andrew V. Martin Marco Kloos Agostino Marinelli Andrew Aquila Thomas R. M. Barends Franz-Josef Decker R. Bruce Doak Lutz Foucar Elisabeth Hartmann Mario Hilpert Mark S. Hunter Zoltan Jurek Jason E. Koglin Alexander Kozlov Alberto A. Lutman Gabriela Nass Kovacs Christopher M. Roome Robert L. Shoeman Robin Santra Harry M. Quiney Beata Ziaja Sébastien Boutet Ilme Schlichting Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses Nature Communications |
| title | Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses |
| title_full | Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses |
| title_fullStr | Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses |
| title_full_unstemmed | Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses |
| title_short | Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses |
| title_sort | structural dynamics in proteins induced by and probed with x ray free electron laser pulses |
| url | https://doi.org/10.1038/s41467-020-15610-4 |
| work_keys_str_mv | AT karolnass structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT alexandergorel structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT malikmabdullah structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT andrewvmartin structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT marcokloos structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT agostinomarinelli structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT andrewaquila structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT thomasrmbarends structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT franzjosefdecker structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT rbrucedoak structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT lutzfoucar structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT elisabethhartmann structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT mariohilpert structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT markshunter structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT zoltanjurek structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT jasonekoglin structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT alexanderkozlov structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT albertoalutman structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT gabrielanasskovacs structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT christophermroome structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT robertlshoeman structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT robinsantra structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT harrymquiney structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT beataziaja structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT sebastienboutet structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses AT ilmeschlichting structuraldynamicsinproteinsinducedbyandprobedwithxrayfreeelectronlaserpulses |