NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer
ABSTRACTAirborne particulate matter (PM) is a major health hazard worldwide and is a key factor in lung cancer, which remains the most common type of malignancy and the leading cause of cancer-related deaths. DNA methylation is a critical mechanism underlying the detrimental effects of PM, however,...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2023.2189934 |
| _version_ | 1797361651244072960 |
|---|---|
| author | Ji Yun Lee Su Man Lee Won Kee Lee Jae Yong Park Dong Sun Kim |
| author_facet | Ji Yun Lee Su Man Lee Won Kee Lee Jae Yong Park Dong Sun Kim |
| author_sort | Ji Yun Lee |
| collection | DOAJ |
| description | ABSTRACTAirborne particulate matter (PM) is a major health hazard worldwide and is a key factor in lung cancer, which remains the most common type of malignancy and the leading cause of cancer-related deaths. DNA methylation is a critical mechanism underlying the detrimental effects of PM, however, the molecular link between PM exposure and lung cancer remains to be elucidated. N-α-acetyltransferase 10 (NAA10) is involved in the cell cycle, migration, apoptosis, differentiation, and proliferation. In order to investigate the role of NAA10 in PM-induced pathogenesis processes leading to lung cancer, we determined the expression and methylation of NAA10 in normal human bronchial epithelial (NHBE) cells treated with PM10, PM10-polycyclic aromatic hydrocarbons (PAH), and PM2.5 and evaluated the prognostic value of the NAA10 methylation status in lung cancer patients. Exposure to all PM types significantly increased the expression of NAA10 mRNA and decreased the methylation of the NAA10 promoter in NHBE cells compared with the mock-treated control. NAA10 hypomethylation was observed in 9.3% (13/140) of lung cancer tissue samples and correlated with NAA10 transcriptional upregulation. Univariate and multivariate analyses revealed that NAA10 hypomethylation was associated with decreased survival of patients with lung cancer. Therefore, these results suggest that PM-induced hypomethylation of the NAA10 may play an important role in the pathogenesis of lung cancer and may be used as a potential prognostic biomarker for lung cancer progression. Further studies with large numbers of patients are warranted to confirm our findings. |
| first_indexed | 2024-03-08T15:57:45Z |
| format | Article |
| id | doaj.art-9afbe577f77e439fbfb9beffd02d2ba8 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-03-08T15:57:45Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-9afbe577f77e439fbfb9beffd02d2ba82024-01-08T17:12:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852023-12-01271728210.1080/19768354.2023.2189934NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancerJi Yun Lee0Su Man Lee1Won Kee Lee2Jae Yong Park3Dong Sun Kim4Department of Anatomy, BK21 Plus Biomedical Convergence Program, Daegu, Republic of KoreaDepartment of Graduate School for Biomedical Science & Engineering, Hanyang University, Seoul, Republic of KoreaDepartment of Preventive Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Anatomy, BK21 Plus Biomedical Convergence Program, Daegu, Republic of KoreaABSTRACTAirborne particulate matter (PM) is a major health hazard worldwide and is a key factor in lung cancer, which remains the most common type of malignancy and the leading cause of cancer-related deaths. DNA methylation is a critical mechanism underlying the detrimental effects of PM, however, the molecular link between PM exposure and lung cancer remains to be elucidated. N-α-acetyltransferase 10 (NAA10) is involved in the cell cycle, migration, apoptosis, differentiation, and proliferation. In order to investigate the role of NAA10 in PM-induced pathogenesis processes leading to lung cancer, we determined the expression and methylation of NAA10 in normal human bronchial epithelial (NHBE) cells treated with PM10, PM10-polycyclic aromatic hydrocarbons (PAH), and PM2.5 and evaluated the prognostic value of the NAA10 methylation status in lung cancer patients. Exposure to all PM types significantly increased the expression of NAA10 mRNA and decreased the methylation of the NAA10 promoter in NHBE cells compared with the mock-treated control. NAA10 hypomethylation was observed in 9.3% (13/140) of lung cancer tissue samples and correlated with NAA10 transcriptional upregulation. Univariate and multivariate analyses revealed that NAA10 hypomethylation was associated with decreased survival of patients with lung cancer. Therefore, these results suggest that PM-induced hypomethylation of the NAA10 may play an important role in the pathogenesis of lung cancer and may be used as a potential prognostic biomarker for lung cancer progression. Further studies with large numbers of patients are warranted to confirm our findings.https://www.tandfonline.com/doi/10.1080/19768354.2023.2189934Particulate matterhuman bronchial epithelial cellslung cancerDNA methylationN-α-acetyltransferase 10 |
| spellingShingle | Ji Yun Lee Su Man Lee Won Kee Lee Jae Yong Park Dong Sun Kim NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer Animal Cells and Systems Particulate matter human bronchial epithelial cells lung cancer DNA methylation N-α-acetyltransferase 10 |
| title | NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer |
| title_full | NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer |
| title_fullStr | NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer |
| title_full_unstemmed | NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer |
| title_short | NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer |
| title_sort | naa10 hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non small cell lung cancer |
| topic | Particulate matter human bronchial epithelial cells lung cancer DNA methylation N-α-acetyltransferase 10 |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2023.2189934 |
| work_keys_str_mv | AT jiyunlee naa10hypomethylationisassociatedwithparticulatematterexposureandworseprognosisforpatientswithnonsmallcelllungcancer AT sumanlee naa10hypomethylationisassociatedwithparticulatematterexposureandworseprognosisforpatientswithnonsmallcelllungcancer AT wonkeelee naa10hypomethylationisassociatedwithparticulatematterexposureandworseprognosisforpatientswithnonsmallcelllungcancer AT jaeyongpark naa10hypomethylationisassociatedwithparticulatematterexposureandworseprognosisforpatientswithnonsmallcelllungcancer AT dongsunkim naa10hypomethylationisassociatedwithparticulatematterexposureandworseprognosisforpatientswithnonsmallcelllungcancer |