Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome
Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biological and medical research, cP-con...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00562/full |
| _version_ | 1819241405955964928 |
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| author | Megumi Shigematsu Takuya Kawamura Yohei Kirino |
| author_facet | Megumi Shigematsu Takuya Kawamura Yohei Kirino |
| author_sort | Megumi Shigematsu |
| collection | DOAJ |
| description | Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biological and medical research, cP-containing RNAs (cP-RNAs) form a hidden transcriptome layer, which is infrequently recognized and characterized, because standard RNA-seq is unable to capture them. Despite cP-RNAs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. This review summarizes our current knowledge of cP-RNA biogenesis pathways and their catalytic enzymatic activities, discusses how the cP-RNA generation affects biological processes, and explores future directions to further investigate cP-RNA biology. |
| first_indexed | 2024-12-23T14:23:24Z |
| format | Article |
| id | doaj.art-9b008fdec8614a9faa462cf829dbbb19 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-23T14:23:24Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9b008fdec8614a9faa462cf829dbbb192022-12-21T17:43:44ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-11-01910.3389/fgene.2018.00562427768Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the TranscriptomeMegumi ShigematsuTakuya KawamuraYohei KirinoCellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biological and medical research, cP-containing RNAs (cP-RNAs) form a hidden transcriptome layer, which is infrequently recognized and characterized, because standard RNA-seq is unable to capture them. Despite cP-RNAs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. This review summarizes our current knowledge of cP-RNA biogenesis pathways and their catalytic enzymatic activities, discusses how the cP-RNA generation affects biological processes, and explores future directions to further investigate cP-RNA biology.https://www.frontiersin.org/article/10.3389/fgene.2018.00562/full2′3′-cyclic phosphate (cP)cP-containing RNA (cP-RNA)cP-RNA-seqribonucleasetRNA half |
| spellingShingle | Megumi Shigematsu Takuya Kawamura Yohei Kirino Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome Frontiers in Genetics 2′ 3′-cyclic phosphate (cP) cP-containing RNA (cP-RNA) cP-RNA-seq ribonuclease tRNA half |
| title | Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome |
| title_full | Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome |
| title_fullStr | Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome |
| title_full_unstemmed | Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome |
| title_short | Generation of 2′,3′-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome |
| title_sort | generation of 2 3 cyclic phosphate containing rnas as a hidden layer of the transcriptome |
| topic | 2′ 3′-cyclic phosphate (cP) cP-containing RNA (cP-RNA) cP-RNA-seq ribonuclease tRNA half |
| url | https://www.frontiersin.org/article/10.3389/fgene.2018.00562/full |
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