Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis
Abstract Background Acetoin (AC) is a vital platform chemical widely used in food, pharmaceutical and chemical industries. With increasing concern over non-renewable resources and environmental issues, using low-cost biomass for acetoin production by microbial fermentation is undoubtedly a promising...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02185-z |
| _version_ | 1811281851775451136 |
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| author | Qiang Wang Xian Zhang Kexin Ren Rumeng Han Ruiqi Lu Teng Bao Xuewei Pan Taowei Yang Meijuan Xu Zhiming Rao |
| author_facet | Qiang Wang Xian Zhang Kexin Ren Rumeng Han Ruiqi Lu Teng Bao Xuewei Pan Taowei Yang Meijuan Xu Zhiming Rao |
| author_sort | Qiang Wang |
| collection | DOAJ |
| description | Abstract Background Acetoin (AC) is a vital platform chemical widely used in food, pharmaceutical and chemical industries. With increasing concern over non-renewable resources and environmental issues, using low-cost biomass for acetoin production by microbial fermentation is undoubtedly a promising strategy. Results This work reduces the disadvantages of Bacillus subtilis during fermentation by regulating genes involved in spore formation and autolysis. Then, optimizing intracellular redox homeostasis through Rex protein mitigated the detrimental effects of NADH produced by the glycolytic metabolic pathway on the process of AC production. Subsequently, multiple pathways that compete with AC production are blocked to optimize carbon flux allocation. Finally, the population cell density-induced promoter was used to enhance the AC synthesis pathway. Fermentation was carried out in a 5-L bioreactor using bagasse lignocellulosic hydrolysate, resulting in a final titer of 64.3 g/L, which was 89.5% of the theoretical yield. Conclusions The recombinant strain BSMAY-4-P srfA provides an economical and efficient strategy for large-scale industrial production of acetoin. |
| first_indexed | 2024-04-13T01:41:02Z |
| format | Article |
| id | doaj.art-9b01ddb37c1a4b3eb6f2af9d11f5e696 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-13T01:41:02Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-9b01ddb37c1a4b3eb6f2af9d11f5e6962022-12-22T03:08:10ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-08-0115111110.1186/s13068-022-02185-zAcetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilisQiang Wang0Xian Zhang1Kexin Ren2Rumeng Han3Ruiqi Lu4Teng Bao5Xuewei Pan6Taowei Yang7Meijuan Xu8Zhiming Rao9Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityDepartment of Bioengineering, The University of Illinois at Urbana-ChampaignKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan UniversityAbstract Background Acetoin (AC) is a vital platform chemical widely used in food, pharmaceutical and chemical industries. With increasing concern over non-renewable resources and environmental issues, using low-cost biomass for acetoin production by microbial fermentation is undoubtedly a promising strategy. Results This work reduces the disadvantages of Bacillus subtilis during fermentation by regulating genes involved in spore formation and autolysis. Then, optimizing intracellular redox homeostasis through Rex protein mitigated the detrimental effects of NADH produced by the glycolytic metabolic pathway on the process of AC production. Subsequently, multiple pathways that compete with AC production are blocked to optimize carbon flux allocation. Finally, the population cell density-induced promoter was used to enhance the AC synthesis pathway. Fermentation was carried out in a 5-L bioreactor using bagasse lignocellulosic hydrolysate, resulting in a final titer of 64.3 g/L, which was 89.5% of the theoretical yield. Conclusions The recombinant strain BSMAY-4-P srfA provides an economical and efficient strategy for large-scale industrial production of acetoin.https://doi.org/10.1186/s13068-022-02185-zAcetoinLow-cost biomassBacillus subtilisCarbon fluxPopulation cell density-induced promoter |
| spellingShingle | Qiang Wang Xian Zhang Kexin Ren Rumeng Han Ruiqi Lu Teng Bao Xuewei Pan Taowei Yang Meijuan Xu Zhiming Rao Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis Biotechnology for Biofuels and Bioproducts Acetoin Low-cost biomass Bacillus subtilis Carbon flux Population cell density-induced promoter |
| title | Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis |
| title_full | Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis |
| title_fullStr | Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis |
| title_full_unstemmed | Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis |
| title_short | Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis |
| title_sort | acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in bacillus subtilis |
| topic | Acetoin Low-cost biomass Bacillus subtilis Carbon flux Population cell density-induced promoter |
| url | https://doi.org/10.1186/s13068-022-02185-z |
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