Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels
Zinc homeostasis is achieved after intake variation by changes in the expression levels of zinc transporters. The aim of this study was to evaluate dietary intake (by 24-h recall), absorption, plasma zinc (by absorption spectrophotometry) and the expression levels (by quantitative PCR), of the trans...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-06-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6643/6/6/2229 |
| _version_ | 1811327191489708032 |
|---|---|
| author | Rosa O. Méndez Alejandra Santiago Gloria Yepiz-Plascencia Alma B. Peregrino-Uriarte Ana M. Calderón de la Barca Hugo S. García |
| author_facet | Rosa O. Méndez Alejandra Santiago Gloria Yepiz-Plascencia Alma B. Peregrino-Uriarte Ana M. Calderón de la Barca Hugo S. García |
| author_sort | Rosa O. Méndez |
| collection | DOAJ |
| description | Zinc homeostasis is achieved after intake variation by changes in the expression levels of zinc transporters. The aim of this study was to evaluate dietary intake (by 24-h recall), absorption, plasma zinc (by absorption spectrophotometry) and the expression levels (by quantitative PCR), of the transporters ZIP1 (zinc importer) and ZnT1 (zinc exporter) in peripheral white blood cells from 24 adolescent girls before and after drinking zinc-fortified milk for 27 day. Zinc intake increased (p < 0.001) from 10.5 ± 3.9 mg/day to 17.6 ± 4.4 mg/day, and its estimated absorption from 3.1 ± 1.2 to 5.3 ± 1.3 mg/day. Mean plasma zinc concentration remained unchanged (p > 0.05) near 150 µg/dL, but increased by 31 µg/dL (p < 0.05) for 6/24 adolescents (group A) and decreased by 25 µg/dL (p < 0.05) for other 6/24 adolescents (group B). Expression of ZIP1 in blood leukocytes was reduced 1.4-fold (p < 0.006) in group A, while for the expression of ZnT1 there was no difference after intervention (p = 0.39). An increase of dietary zinc after 27-days consumption of fortified-milk did not increase (p > 0.05) the plasma level of adolescent girls but for 6/24 participants from group A in spite of the formerly appropriation, which cellular zinc uptake decreased as assessed by reduction of the expression of ZIP1. |
| first_indexed | 2024-04-13T15:03:29Z |
| format | Article |
| id | doaj.art-9b045f2af57b47c18f840dff9ef4421a |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-13T15:03:29Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-9b045f2af57b47c18f840dff9ef4421a2022-12-22T02:42:14ZengMDPI AGNutrients2072-66432014-06-01662229223910.3390/nu6062229nu6062229Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc LevelsRosa O. Méndez0Alejandra Santiago1Gloria Yepiz-Plascencia2Alma B. Peregrino-Uriarte3Ana M. Calderón de la Barca4Hugo S. García5Research Center for Food and Development (CIAD), Km 0.6 a La Victoria, Hermosillo, Sonora 83304, MexicoResearch Center for Food and Development (CIAD), Km 0.6 a La Victoria, Hermosillo, Sonora 83304, MexicoResearch Center for Food and Development (CIAD), Km 0.6 a La Victoria, Hermosillo, Sonora 83304, MexicoResearch Center for Food and Development (CIAD), Km 0.6 a La Victoria, Hermosillo, Sonora 83304, MexicoResearch Center for Food and Development (CIAD), Km 0.6 a La Victoria, Hermosillo, Sonora 83304, MexicoTechnological Institute of Veracruz, UNIDA, Calzada Miguel Ángel de Quevedo 2779, Veracruz, Veracruz 91897, MexicoZinc homeostasis is achieved after intake variation by changes in the expression levels of zinc transporters. The aim of this study was to evaluate dietary intake (by 24-h recall), absorption, plasma zinc (by absorption spectrophotometry) and the expression levels (by quantitative PCR), of the transporters ZIP1 (zinc importer) and ZnT1 (zinc exporter) in peripheral white blood cells from 24 adolescent girls before and after drinking zinc-fortified milk for 27 day. Zinc intake increased (p < 0.001) from 10.5 ± 3.9 mg/day to 17.6 ± 4.4 mg/day, and its estimated absorption from 3.1 ± 1.2 to 5.3 ± 1.3 mg/day. Mean plasma zinc concentration remained unchanged (p > 0.05) near 150 µg/dL, but increased by 31 µg/dL (p < 0.05) for 6/24 adolescents (group A) and decreased by 25 µg/dL (p < 0.05) for other 6/24 adolescents (group B). Expression of ZIP1 in blood leukocytes was reduced 1.4-fold (p < 0.006) in group A, while for the expression of ZnT1 there was no difference after intervention (p = 0.39). An increase of dietary zinc after 27-days consumption of fortified-milk did not increase (p > 0.05) the plasma level of adolescent girls but for 6/24 participants from group A in spite of the formerly appropriation, which cellular zinc uptake decreased as assessed by reduction of the expression of ZIP1.http://www.mdpi.com/2072-6643/6/6/2229adolescent girlszinc fortified milkzinc intakeplasma zinczinc transporter expression |
| spellingShingle | Rosa O. Méndez Alejandra Santiago Gloria Yepiz-Plascencia Alma B. Peregrino-Uriarte Ana M. Calderón de la Barca Hugo S. García Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels Nutrients adolescent girls zinc fortified milk zinc intake plasma zinc zinc transporter expression |
| title | Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels |
| title_full | Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels |
| title_fullStr | Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels |
| title_full_unstemmed | Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels |
| title_short | Zinc Fortification Decreases ZIP1 Gene Expression of Some Adolescent Females with Appropriate Plasma Zinc Levels |
| title_sort | zinc fortification decreases zip1 gene expression of some adolescent females with appropriate plasma zinc levels |
| topic | adolescent girls zinc fortified milk zinc intake plasma zinc zinc transporter expression |
| url | http://www.mdpi.com/2072-6643/6/6/2229 |
| work_keys_str_mv | AT rosaomendez zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels AT alejandrasantiago zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels AT gloriayepizplascencia zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels AT almabperegrinouriarte zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels AT anamcalderondelabarca zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels AT hugosgarcia zincfortificationdecreaseszip1geneexpressionofsomeadolescentfemaleswithappropriateplasmazinclevels |