Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few report...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/22/12515 |
| Summary: | The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from <i>Glycine max</i> were conducted. The results indicated that the <i>GmLOR</i> gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated <i>GmLORs</i> and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of <i>GmLOR</i> genes. The expansion patterns of the <i>GmLOR</i> family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 <i>GmLOR</i> genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of <i>GmLORs</i> include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of <i>GmLOR</i> genes, and most of them were expressed in the leaf or root except that <i>GmLOR6</i> was induced by osmotic and salt stresses. Moreover, <i>GmLOR4/10/14/19</i> were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of <i>GmLOR</i> gene functions in soybean. |
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| ISSN: | 1661-6596 1422-0067 |