Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few report...
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2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12515 |
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| author | Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou |
| author_facet | Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou |
| author_sort | Yisheng Fang |
| collection | DOAJ |
| description | The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from <i>Glycine max</i> were conducted. The results indicated that the <i>GmLOR</i> gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated <i>GmLORs</i> and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of <i>GmLOR</i> genes. The expansion patterns of the <i>GmLOR</i> family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 <i>GmLOR</i> genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of <i>GmLORs</i> include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of <i>GmLOR</i> genes, and most of them were expressed in the leaf or root except that <i>GmLOR6</i> was induced by osmotic and salt stresses. Moreover, <i>GmLOR4/10/14/19</i> were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of <i>GmLOR</i> gene functions in soybean. |
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| issn | 1661-6596 1422-0067 |
| language | English |
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| publishDate | 2021-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9b04fe7e54a94d6796a1fe8c338e85152023-11-22T23:43:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221251510.3390/ijms222212515Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic StressesYisheng Fang0Dong Cao1Hongli Yang2Wei Guo3Wenqi Ouyang4Haifeng Chen5Zhihui Shan6Zhonglu Yang7Shuilian Chen8Xia Li9Limiao Chen10Xinan Zhou11Key laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Hongshan District, Wuhan 430070, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaThe LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from <i>Glycine max</i> were conducted. The results indicated that the <i>GmLOR</i> gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated <i>GmLORs</i> and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of <i>GmLOR</i> genes. The expansion patterns of the <i>GmLOR</i> family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 <i>GmLOR</i> genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of <i>GmLORs</i> include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of <i>GmLOR</i> genes, and most of them were expressed in the leaf or root except that <i>GmLOR6</i> was induced by osmotic and salt stresses. Moreover, <i>GmLOR4/10/14/19</i> were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of <i>GmLOR</i> gene functions in soybean.https://www.mdpi.com/1422-0067/22/22/12515soybeanLOR gene familyphylogenetic analysisgene expressionosmotic stresssalt stress |
| spellingShingle | Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses International Journal of Molecular Sciences soybean LOR gene family phylogenetic analysis gene expression osmotic stress salt stress |
| title | Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses |
| title_full | Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses |
| title_fullStr | Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses |
| title_full_unstemmed | Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses |
| title_short | Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses |
| title_sort | genome wide identification and characterization of soybean i gmlor i gene family and expression analysis in response to abiotic stresses |
| topic | soybean LOR gene family phylogenetic analysis gene expression osmotic stress salt stress |
| url | https://www.mdpi.com/1422-0067/22/22/12515 |
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