Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors
The objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these flocks an...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/10/1016 |
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| author | Daphne T. Lianou Efthymia Petinaki Peter J. Cripps Dimitris A. Gougoulis Charalambia K. Michael Katerina Tsilipounidaki Anargyros Skoulakis Angeliki I. Katsafadou Natalia G. C. Vasileiou Themis Giannoulis Chrysoula Voidarou Vasia S. Mavrogianni Mariangela Caroprese George C. Fthenakis |
| author_facet | Daphne T. Lianou Efthymia Petinaki Peter J. Cripps Dimitris A. Gougoulis Charalambia K. Michael Katerina Tsilipounidaki Anargyros Skoulakis Angeliki I. Katsafadou Natalia G. C. Vasileiou Themis Giannoulis Chrysoula Voidarou Vasia S. Mavrogianni Mariangela Caroprese George C. Fthenakis |
| author_sort | Daphne T. Lianou |
| collection | DOAJ |
| description | The objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these flocks and to evaluate flock-related factors potentially associated with antibiotic resistance among these isolates. A cross-sectional study was performed in 325 sheep flocks in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant staphylococcal isolates, isolates resistant to any antibiotic, and multi-resistant isolates were recovered from 8.0%, 30.5%, and 12.0% of flocks, respectively. Of 232 isolates, 11.6% were resistant to oxacillin, 46.1% were resistant to at least one antibiotic, and 16.4% were multi-resistant. Resistance was seen more frequently among coagulase-negative (50.6%) than among <i>Staphylococcus aureus</i> (31.5%) isolates. Resistance was more frequent against penicillin and ampicillin (34.1% of isolates), clindamycin (17.7%), and fosfomycin (14.2%). An association was found between biofilm formation by staphylococci and resistance to fosfomycin. For recovery of oxacillin-resistant isolates, the lack of experience by farmers emerged as a significant factor; respective factors for the isolation of staphylococci resistant to any antibiotic or multi-resistant isolates were the early stage of the lactation period (0th–1st month) and the intensive management system applied in the flocks, respectively. |
| first_indexed | 2024-03-10T06:43:37Z |
| format | Article |
| id | doaj.art-9b0bda8bc0104ce59a5abfdde2d5684a |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T06:43:37Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-9b0bda8bc0104ce59a5abfdde2d5684a2023-11-22T17:28:28ZengMDPI AGBiology2079-77372021-10-011010101610.3390/biology10101016Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk FactorsDaphne T. Lianou0Efthymia Petinaki1Peter J. Cripps2Dimitris A. Gougoulis3Charalambia K. Michael4Katerina Tsilipounidaki5Anargyros Skoulakis6Angeliki I. Katsafadou7Natalia G. C. Vasileiou8Themis Giannoulis9Chrysoula Voidarou10Vasia S. Mavrogianni11Mariangela Caroprese12George C. Fthenakis13Veterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceFaculty of Public and One Health, University of Thessaly, 43100 Karditsa, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larissa, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larissa, GreeceDepartment of Agriculture, University of Ioannina, 47132 Arta, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceDepartment of Agriculture, Food, Natural Resources and Engineering (DAFNE), University of Foggia, 71122 Foggia, ItalyVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceThe objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these flocks and to evaluate flock-related factors potentially associated with antibiotic resistance among these isolates. A cross-sectional study was performed in 325 sheep flocks in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant staphylococcal isolates, isolates resistant to any antibiotic, and multi-resistant isolates were recovered from 8.0%, 30.5%, and 12.0% of flocks, respectively. Of 232 isolates, 11.6% were resistant to oxacillin, 46.1% were resistant to at least one antibiotic, and 16.4% were multi-resistant. Resistance was seen more frequently among coagulase-negative (50.6%) than among <i>Staphylococcus aureus</i> (31.5%) isolates. Resistance was more frequent against penicillin and ampicillin (34.1% of isolates), clindamycin (17.7%), and fosfomycin (14.2%). An association was found between biofilm formation by staphylococci and resistance to fosfomycin. For recovery of oxacillin-resistant isolates, the lack of experience by farmers emerged as a significant factor; respective factors for the isolation of staphylococci resistant to any antibiotic or multi-resistant isolates were the early stage of the lactation period (0th–1st month) and the intensive management system applied in the flocks, respectively.https://www.mdpi.com/2079-7737/10/10/1016bulk-tank milkmastitismethicillinmilksheepsomatic cell counts |
| spellingShingle | Daphne T. Lianou Efthymia Petinaki Peter J. Cripps Dimitris A. Gougoulis Charalambia K. Michael Katerina Tsilipounidaki Anargyros Skoulakis Angeliki I. Katsafadou Natalia G. C. Vasileiou Themis Giannoulis Chrysoula Voidarou Vasia S. Mavrogianni Mariangela Caroprese George C. Fthenakis Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors Biology bulk-tank milk mastitis methicillin milk sheep somatic cell counts |
| title | Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors |
| title_full | Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors |
| title_fullStr | Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors |
| title_full_unstemmed | Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors |
| title_short | Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors |
| title_sort | antibiotic resistance of staphylococci from bulk tank milk of sheep flocks prevalence patterns association with biofilm formation effects on milk quality and risk factors |
| topic | bulk-tank milk mastitis methicillin milk sheep somatic cell counts |
| url | https://www.mdpi.com/2079-7737/10/10/1016 |
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