Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation
Rheumatoid arthritis is a chronic inflammatory autoimmune disease, causing articular and extra-articular dysfunctions among patients, and it could result in irreversible joint damages or disability if untreated. A traditional Chinese medicine formula, Huayu-Qiangshen-Tongbi (HT) formula, has been ob...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.01065/full |
| _version_ | 1818179552671694848 |
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| author | Zihao Wang Ke-Gang Linghu Yuanjia Hu Huali Zuo Hao Yi Shi-Hang Xiong Jinjian Lu Ging Chan Hua Yu Hua Yu Hua Yu Run-Yue Huang Run-Yue Huang |
| author_facet | Zihao Wang Ke-Gang Linghu Yuanjia Hu Huali Zuo Hao Yi Shi-Hang Xiong Jinjian Lu Ging Chan Hua Yu Hua Yu Hua Yu Run-Yue Huang Run-Yue Huang |
| author_sort | Zihao Wang |
| collection | DOAJ |
| description | Rheumatoid arthritis is a chronic inflammatory autoimmune disease, causing articular and extra-articular dysfunctions among patients, and it could result in irreversible joint damages or disability if untreated. A traditional Chinese medicine formula, Huayu-Qiangshen-Tongbi (HT) formula, has been observed successful in controlling rheumatoid arthritis progression in traditional Chinese medicine clinics. In this study, we conducted a systematic analysis of the HT formula with a purpose of proposing for its potential mechanism of action using network pharmacological methods. The potential targets of the formula were collected and screened according to the topological features of their protein–protein interaction network, and we subsequently validated our prediction results through in vitro experiments. We proposed that the HT formula could interfere with the bone metabolism and the inflammatory pathways of the body. The experimental validation results indicated that HT formula could exhibit anti-inflammatory effects by regulating several signaling pathways specifically the Toll-like receptor signaling pathway, phosphoinositide-3-kinase–Akt signaling pathway, hypoxia-inducible factor 1 signaling pathway, mitogen-activated protein kinase signaling pathway and activator protein 1 signaling pathway. |
| first_indexed | 2024-12-11T21:05:41Z |
| format | Article |
| id | doaj.art-9b0de782e4474098919d6899d7d0414d |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T21:05:41Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-9b0de782e4474098919d6899d7d0414d2022-12-22T00:50:52ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-09-011010.3389/fphar.2019.01065460863Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological InvestigationZihao Wang0Ke-Gang Linghu1Yuanjia Hu2Huali Zuo3Hao Yi4Shi-Hang Xiong5Jinjian Lu6Ging Chan7Hua Yu8Hua Yu9Hua Yu10Run-Yue Huang11Run-Yue Huang12State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaDepartment of Rheumatology and Immunology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaHKBU Shenzhen Research Center, Shenzhen, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong, Hong KongDepartment of Rheumatology and Immunology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou, ChinaRheumatoid arthritis is a chronic inflammatory autoimmune disease, causing articular and extra-articular dysfunctions among patients, and it could result in irreversible joint damages or disability if untreated. A traditional Chinese medicine formula, Huayu-Qiangshen-Tongbi (HT) formula, has been observed successful in controlling rheumatoid arthritis progression in traditional Chinese medicine clinics. In this study, we conducted a systematic analysis of the HT formula with a purpose of proposing for its potential mechanism of action using network pharmacological methods. The potential targets of the formula were collected and screened according to the topological features of their protein–protein interaction network, and we subsequently validated our prediction results through in vitro experiments. We proposed that the HT formula could interfere with the bone metabolism and the inflammatory pathways of the body. The experimental validation results indicated that HT formula could exhibit anti-inflammatory effects by regulating several signaling pathways specifically the Toll-like receptor signaling pathway, phosphoinositide-3-kinase–Akt signaling pathway, hypoxia-inducible factor 1 signaling pathway, mitogen-activated protein kinase signaling pathway and activator protein 1 signaling pathway.https://www.frontiersin.org/article/10.3389/fphar.2019.01065/fullrheumatoid arthritisnetwork pharmacologytraditional Chinese medicineHuayu-Qiangshen-Tongbi formulain vitro validation |
| spellingShingle | Zihao Wang Ke-Gang Linghu Yuanjia Hu Huali Zuo Hao Yi Shi-Hang Xiong Jinjian Lu Ging Chan Hua Yu Hua Yu Hua Yu Run-Yue Huang Run-Yue Huang Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation Frontiers in Pharmacology rheumatoid arthritis network pharmacology traditional Chinese medicine Huayu-Qiangshen-Tongbi formula in vitro validation |
| title | Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation |
| title_full | Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation |
| title_fullStr | Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation |
| title_full_unstemmed | Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation |
| title_short | Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation |
| title_sort | deciphering the pharmacological mechanisms of the huayu qiangshen tongbi formula through integrating network pharmacology and in vitro pharmacological investigation |
| topic | rheumatoid arthritis network pharmacology traditional Chinese medicine Huayu-Qiangshen-Tongbi formula in vitro validation |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.01065/full |
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