Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs
Abstract In dogs, Porphyromonas gulae is a major periodontal pathogen with 41-kDa proteins polymerizing to form a filamentous structure called fimbriae or pili, termed FimA. FimA is classified into three genotypes: A, B, and C, and there are combinations of types A, B, C, A/B, A/C, B/C, and A/B/C. P...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-55842-8 |
| _version_ | 1797274946360049664 |
|---|---|
| author | Junya Yasuda Hidemi Yasuda Ryota Nomura Saaya Matayoshi Hiroaki Inaba Enrique Gongora Naoki Iwashita So Shirahata Noriyuki Kaji Tatsuya Akitomo Chieko Mitsuhata Jumpei Uchiyama Tomoki Fukuyama Michiyo Matsumoto-Nakano Kazuhiko Nakano Masaru Murakami |
| author_facet | Junya Yasuda Hidemi Yasuda Ryota Nomura Saaya Matayoshi Hiroaki Inaba Enrique Gongora Naoki Iwashita So Shirahata Noriyuki Kaji Tatsuya Akitomo Chieko Mitsuhata Jumpei Uchiyama Tomoki Fukuyama Michiyo Matsumoto-Nakano Kazuhiko Nakano Masaru Murakami |
| author_sort | Junya Yasuda |
| collection | DOAJ |
| description | Abstract In dogs, Porphyromonas gulae is a major periodontal pathogen with 41-kDa proteins polymerizing to form a filamentous structure called fimbriae or pili, termed FimA. FimA is classified into three genotypes: A, B, and C, and there are combinations of types A, B, C, A/B, A/C, B/C, and A/B/C. Periodontal disease is the most common oral disease in small dogs, but the periodontal disease status and P. gulae colonization at each dog age and breed remain unclear. In this study, we stratified 665 small dogs and analyzed the periodontal status and distribution of P. gulae with each FimA genotype. Dogs with periodontal disease and FimA genotype tended to increase with age. The dogs with at least one FimA genotype had significantly more severe periodontal disease compared with P. gulae-negative dogs (P < 0.01). Additionally, periodontal status was significantly associated with specific FimA genotype distribution in Toy Poodles and Chihuahuas (P < 0.05), whereas there was no such association in Dachshunds. These results suggest that the onset of periodontal disease and P. gulae colonization are related and progress with age. The relationship between periodontal disease and FimA genotype may differ depending on the dog breeds. |
| first_indexed | 2024-03-07T15:06:22Z |
| format | Article |
| id | doaj.art-9b0f4fd54c394509b4ccde8be7865ae5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:06:22Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9b0f4fd54c394509b4ccde8be7865ae52024-03-05T18:54:03ZengNature PortfolioScientific Reports2045-23222024-03-0114111010.1038/s41598-024-55842-8Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogsJunya Yasuda0Hidemi Yasuda1Ryota Nomura2Saaya Matayoshi3Hiroaki Inaba4Enrique Gongora5Naoki Iwashita6So Shirahata7Noriyuki Kaji8Tatsuya Akitomo9Chieko Mitsuhata10Jumpei Uchiyama11Tomoki Fukuyama12Michiyo Matsumoto-Nakano13Kazuhiko Nakano14Masaru Murakami15Department of Molecular Biology, School of Veterinary Medicine, Azabu UniversityYasuda Veterinary ClinicDepartment of Pediatric Dentistry, Osaka University Graduate School of DentistryDepartment of Pediatric Dentistry, Osaka University Graduate School of DentistryDepartment of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesYasuda Veterinary ClinicDepartment of Pharmacology, School of Veterinary Medicine, Azabu UniversityDepartment of Pharmacology, School of Veterinary Medicine, Azabu UniversityDepartment of Pharmacology, School of Veterinary Medicine, Azabu UniversityDepartment of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Pharmacology, School of Veterinary Medicine, Azabu UniversityDepartment of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Pediatric Dentistry, Osaka University Graduate School of DentistryDepartment of Molecular Biology, School of Veterinary Medicine, Azabu UniversityAbstract In dogs, Porphyromonas gulae is a major periodontal pathogen with 41-kDa proteins polymerizing to form a filamentous structure called fimbriae or pili, termed FimA. FimA is classified into three genotypes: A, B, and C, and there are combinations of types A, B, C, A/B, A/C, B/C, and A/B/C. Periodontal disease is the most common oral disease in small dogs, but the periodontal disease status and P. gulae colonization at each dog age and breed remain unclear. In this study, we stratified 665 small dogs and analyzed the periodontal status and distribution of P. gulae with each FimA genotype. Dogs with periodontal disease and FimA genotype tended to increase with age. The dogs with at least one FimA genotype had significantly more severe periodontal disease compared with P. gulae-negative dogs (P < 0.01). Additionally, periodontal status was significantly associated with specific FimA genotype distribution in Toy Poodles and Chihuahuas (P < 0.05), whereas there was no such association in Dachshunds. These results suggest that the onset of periodontal disease and P. gulae colonization are related and progress with age. The relationship between periodontal disease and FimA genotype may differ depending on the dog breeds.https://doi.org/10.1038/s41598-024-55842-8 |
| spellingShingle | Junya Yasuda Hidemi Yasuda Ryota Nomura Saaya Matayoshi Hiroaki Inaba Enrique Gongora Naoki Iwashita So Shirahata Noriyuki Kaji Tatsuya Akitomo Chieko Mitsuhata Jumpei Uchiyama Tomoki Fukuyama Michiyo Matsumoto-Nakano Kazuhiko Nakano Masaru Murakami Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs Scientific Reports |
| title | Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs |
| title_full | Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs |
| title_fullStr | Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs |
| title_full_unstemmed | Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs |
| title_short | Investigation of periodontal disease development and Porphyromonas gulae FimA genotype distribution in small dogs |
| title_sort | investigation of periodontal disease development and porphyromonas gulae fima genotype distribution in small dogs |
| url | https://doi.org/10.1038/s41598-024-55842-8 |
| work_keys_str_mv | AT junyayasuda investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT hidemiyasuda investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT ryotanomura investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT saayamatayoshi investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT hiroakiinaba investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT enriquegongora investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT naokiiwashita investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT soshirahata investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT noriyukikaji investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT tatsuyaakitomo investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT chiekomitsuhata investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT jumpeiuchiyama investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT tomokifukuyama investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT michiyomatsumotonakano investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT kazuhikonakano investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs AT masarumurakami investigationofperiodontaldiseasedevelopmentandporphyromonasgulaefimagenotypedistributioninsmalldogs |