Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China
In October 2017, a small outbreak of echovirus 30 (E30) associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region. That year, we observed a significant increase in E30 levels in an acute flaccid paralysis (AFP) case surveillance system. To inv...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biosafety and Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590053623001258 |
| _version_ | 1797378617928318976 |
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| author | Fang Yuan Xinfeng Wei Xueping Ma Jiangtao Ma Xuemin Ma Xiaoqiang Sun Min Cao Juan Zhou Wei Zhang Hui Chen Rui Wang Jichen Li Qiang Sun |
| author_facet | Fang Yuan Xinfeng Wei Xueping Ma Jiangtao Ma Xuemin Ma Xiaoqiang Sun Min Cao Juan Zhou Wei Zhang Hui Chen Rui Wang Jichen Li Qiang Sun |
| author_sort | Fang Yuan |
| collection | DOAJ |
| description | In October 2017, a small outbreak of echovirus 30 (E30) associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region. That year, we observed a significant increase in E30 levels in an acute flaccid paralysis (AFP) case surveillance system. To investigate their phylogenetic relationships, we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases, AFP cases, and healthy children. We found that the E30 strains circulating in Ningxia belong to two lineages (H and J). The strains isolated in 2010, 2012, and 2016 belonged to the H lineage. In 2017, a new lineage, J, emerged as the dominant lineage. Phylogenetic trees were constructed based on the whole genome and P1, P2, and P3 regions; clustering with other types of enterovirus species B was found, suggesting that recombination events had occurred. The recombination sites were mainly in regions 2B, 2C, and 3D. This study confirmed that the E30 strains in Ningxia in 2010, 2012, and 2016 had different recombination patterns and were recombined with different enteroviruses. The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia. |
| first_indexed | 2024-03-08T20:10:17Z |
| format | Article |
| id | doaj.art-9b1192019cfe44fcadb0691cba69d50e |
| institution | Directory Open Access Journal |
| issn | 2590-0536 |
| language | English |
| last_indexed | 2024-03-08T20:10:17Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosafety and Health |
| spelling | doaj.art-9b1192019cfe44fcadb0691cba69d50e2023-12-23T05:22:32ZengElsevierBiosafety and Health2590-05362023-12-0156346354Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, ChinaFang Yuan0Xinfeng Wei1Xueping Ma2Jiangtao Ma3Xuemin Ma4Xiaoqiang Sun5Min Cao6Juan Zhou7Wei Zhang8Hui Chen9Rui Wang10Jichen Li11Qiang Sun12Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region People's Hospital, Yinchuan 750021, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, ChinaNational Laboratory for Poliomyelitis, WHO WPRO Regional Polio Reference Laboratory, National Key Laboratory for Traceability, Early Warning and Intelligent Decision of Infectious Diseases, National Health Commission Key Laboratory for Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Laboratory for Poliomyelitis, WHO WPRO Regional Polio Reference Laboratory, National Key Laboratory for Traceability, Early Warning and Intelligent Decision of Infectious Diseases, National Health Commission Key Laboratory for Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Laboratory for Poliomyelitis, WHO WPRO Regional Polio Reference Laboratory, National Key Laboratory for Traceability, Early Warning and Intelligent Decision of Infectious Diseases, National Health Commission Key Laboratory for Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Corresponding author: National Laboratory for Poliomyelitis, WHO WPRO Regional Polio Reference Laboratory, National Key Laboratory for Traceability, Early Warning and Intelligent Decision of Infectious Diseases, National Health Commission Key Laboratory for Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.In October 2017, a small outbreak of echovirus 30 (E30) associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region. That year, we observed a significant increase in E30 levels in an acute flaccid paralysis (AFP) case surveillance system. To investigate their phylogenetic relationships, we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases, AFP cases, and healthy children. We found that the E30 strains circulating in Ningxia belong to two lineages (H and J). The strains isolated in 2010, 2012, and 2016 belonged to the H lineage. In 2017, a new lineage, J, emerged as the dominant lineage. Phylogenetic trees were constructed based on the whole genome and P1, P2, and P3 regions; clustering with other types of enterovirus species B was found, suggesting that recombination events had occurred. The recombination sites were mainly in regions 2B, 2C, and 3D. This study confirmed that the E30 strains in Ningxia in 2010, 2012, and 2016 had different recombination patterns and were recombined with different enteroviruses. The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia.http://www.sciencedirect.com/science/article/pii/S2590053623001258Echovirus 30 (E30)Complete genome sequenceMolecular epidemiology |
| spellingShingle | Fang Yuan Xinfeng Wei Xueping Ma Jiangtao Ma Xuemin Ma Xiaoqiang Sun Min Cao Juan Zhou Wei Zhang Hui Chen Rui Wang Jichen Li Qiang Sun Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China Biosafety and Health Echovirus 30 (E30) Complete genome sequence Molecular epidemiology |
| title | Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China |
| title_full | Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China |
| title_fullStr | Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China |
| title_full_unstemmed | Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China |
| title_short | Genetic characterizations and molecular epidemiology of human echovirus 30 isolated from Ningxia, China |
| title_sort | genetic characterizations and molecular epidemiology of human echovirus 30 isolated from ningxia china |
| topic | Echovirus 30 (E30) Complete genome sequence Molecular epidemiology |
| url | http://www.sciencedirect.com/science/article/pii/S2590053623001258 |
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