Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex
This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme ac...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2015-12-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/24200 |
| _version_ | 1818084701227712512 |
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| author | Sri Wahyuni R. Susanti Retno Sri Iswari |
| author_facet | Sri Wahyuni R. Susanti Retno Sri Iswari |
| author_sort | Sri Wahyuni |
| collection | DOAJ |
| description | This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8. |
| first_indexed | 2024-12-10T19:58:04Z |
| format | Article |
| id | doaj.art-9b125ec9a2534f6db8d6de39c4190bbc |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-10T19:58:04Z |
| publishDate | 2015-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-9b125ec9a2534f6db8d6de39c4190bbc2022-12-22T01:35:37ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-12-0120216116610.22146/ijbiotech.2420016168Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latexSri Wahyuni0R. Susanti1Retno Sri Iswari2Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, IndonesiaBiology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, IndonesiaBiology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, IndonesiaThis research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.https://jurnal.ugm.ac.id/ijbiotech/article/view/24200Ficus septica Burm Fficin enzymetemperaturepH |
| spellingShingle | Sri Wahyuni R. Susanti Retno Sri Iswari Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex Indonesian Journal of Biotechnology Ficus septica Burm F ficin enzyme temperature pH |
| title | Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex |
| title_full | Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex |
| title_fullStr | Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex |
| title_full_unstemmed | Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex |
| title_short | Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex |
| title_sort | isolation and caracterization of ficin enzyme from ficus septica burm f stem latex |
| topic | Ficus septica Burm F ficin enzyme temperature pH |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/24200 |
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