Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats
Background: Tamarindus indica (TI) extract is known to possess important phytochemical composition. The study aimed to assess the effects of ethyl acetate fraction of TI leaves (EATI) on memory and learning, traced element and brain sialic acid levels, and hippocampal Nissl substance expression foll...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Trace Elements and Minerals |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773050623000022 |
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| author | Ibe Michael Usman, PhD Abel Nosereme Agbon, PhD Andrew Ekpeyong Ivang, PhD Akwu Bala Peter Adam Moyosore Afodun, PhD Akeem Ayodeji Okesina, PhD Victor Fischer, PhD Bot Yakubu Sunday, PhD Eric Osamu Aigbogun, Jr., PhD Ismail Onanuga, PhD Caroline Asekenye, MSc Ann Monima Lemuel, MSc Keneth Iceland Kasozi, PhD |
| author_facet | Ibe Michael Usman, PhD Abel Nosereme Agbon, PhD Andrew Ekpeyong Ivang, PhD Akwu Bala Peter Adam Moyosore Afodun, PhD Akeem Ayodeji Okesina, PhD Victor Fischer, PhD Bot Yakubu Sunday, PhD Eric Osamu Aigbogun, Jr., PhD Ismail Onanuga, PhD Caroline Asekenye, MSc Ann Monima Lemuel, MSc Keneth Iceland Kasozi, PhD |
| author_sort | Ibe Michael Usman, PhD |
| collection | DOAJ |
| description | Background: Tamarindus indica (TI) extract is known to possess important phytochemical composition. The study aimed to assess the effects of ethyl acetate fraction of TI leaves (EATI) on memory and learning, traced element and brain sialic acid levels, and hippocampal Nissl substance expression following prenatal (PN) AlCl3 exposure in Wistar rat pups. Methods: Pregnant rats were divided into 5 groups (n = 4); Group I received distilled water (negative control), Group II-V were experimental groups treated with 200 mg/kg of AlCl3. Group II (positive control), Groups III, and IV received 400 mg kg−1 and 800 mg kg−1 of EATI, respectively, and Group V received 300 mg kg−1 of Vitamin E (comparatively control) for 2 weeks (PN day 7–21). On postnatal (PoN) day 19, male pups were subjected to novel object recognition test (n = 5). Some pups were euthanized on PoN days 7 and 21; and samples were collected for quantification of trace element (n = 5), brain sialic acid concentration (n = 5), and Nissl staining using cresyl fast (n = 3). Results: The results of the trace elements quantification on PoN days 7 and 21 revealed significantly lower mean copper, zinc, and iron, and significantly higher calcium level in the group treated with 400 and 800 mg kg−1 of EATI when compared with the positive control group. EATI at a dose of 800 mg kg−1 showed improvement in memory and increased brain sialic acid. EATI 400 mg kg−1 increased only free sialic acid and had no effect on memory. Improvement in Nissl staining was observed in the EATI treated group on PoN day 7 and 21. Conclusion: The administration of EATI ameliorated AlCl3-induced neural toxicity. |
| first_indexed | 2024-04-10T15:47:38Z |
| format | Article |
| id | doaj.art-9b1411d78d2d4a9ab74e3a16c0ed30d2 |
| institution | Directory Open Access Journal |
| issn | 2773-0506 |
| language | English |
| last_indexed | 2024-04-10T15:47:38Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
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| series | Journal of Trace Elements and Minerals |
| spelling | doaj.art-9b1411d78d2d4a9ab74e3a16c0ed30d22023-02-12T04:15:56ZengElsevierJournal of Trace Elements and Minerals2773-05062023-03-013100047Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar ratsIbe Michael Usman, PhD0Abel Nosereme Agbon, PhD1Andrew Ekpeyong Ivang, PhD2Akwu Bala Peter3Adam Moyosore Afodun, PhD4Akeem Ayodeji Okesina, PhD5Victor Fischer, PhD6Bot Yakubu Sunday, PhD7Eric Osamu Aigbogun, Jr., PhD8Ismail Onanuga, PhD9Caroline Asekenye, MSc10Ann Monima Lemuel, MSc11Keneth Iceland Kasozi, PhD12Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Uganda; Corresponding author.Department of Human Anatomy, Ahmadu Bello University, Zaria, NigeriaDepartment of Human Anatomy, University of Rwanda, Kigali, RwandaDepartment of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Uganda; Department of Human Anatomy, Ahmadu Bello University, Zaria, Nigeria; Department of Human Anatomy, University of Rwanda, Kigali, Rwanda; Department of Medical Laboratory Sciences, Kampala International University, Uganda; Department of Human Anatomy, Enugu State University of Science and Technology, Enugu, Nigeria; Department of Human Anatomy, Kampala International University, Tanzania; Department of Pharmacognosy and Natural Substances, Kampala International University, Uganda; School of Medicine, Kabale University, Kabale, Uganda; Department of Anatomy & Cell Biology, Faculty of Health Sciences, Busitema University, Uganda; Department of Clinical Medicine and Community Health, University of Rwanda.Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Uganda; Department of Human Anatomy, Ahmadu Bello University, Zaria, Nigeria; Department of Human Anatomy, University of Rwanda, Kigali, Rwanda; Department of Medical Laboratory Sciences, Kampala International University, Uganda; Department of Human Anatomy, Enugu State University of Science and Technology, Enugu, Nigeria; Department of Human Anatomy, Kampala International University, Tanzania; Department of Pharmacognosy and Natural Substances, Kampala International University, Uganda; School of Medicine, Kabale University, Kabale, Uganda; Department of Anatomy & Cell Biology, Faculty of Health Sciences, Busitema University, Uganda; Department of Clinical Medicine and Community Health, University of Rwanda.Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Medical Laboratory Sciences, Kampala International University, UgandaDepartment of Human Anatomy, Enugu State University of Science and Technology, Enugu, NigeriaDepartment of Human Anatomy, Kampala International University, TanzaniaDepartment of Pharmacognosy and Natural Substances, Kampala International University, UgandaDepartment of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, UgandaSchool of Medicine, Kabale University, Kabale, UgandaBackground: Tamarindus indica (TI) extract is known to possess important phytochemical composition. The study aimed to assess the effects of ethyl acetate fraction of TI leaves (EATI) on memory and learning, traced element and brain sialic acid levels, and hippocampal Nissl substance expression following prenatal (PN) AlCl3 exposure in Wistar rat pups. Methods: Pregnant rats were divided into 5 groups (n = 4); Group I received distilled water (negative control), Group II-V were experimental groups treated with 200 mg/kg of AlCl3. Group II (positive control), Groups III, and IV received 400 mg kg−1 and 800 mg kg−1 of EATI, respectively, and Group V received 300 mg kg−1 of Vitamin E (comparatively control) for 2 weeks (PN day 7–21). On postnatal (PoN) day 19, male pups were subjected to novel object recognition test (n = 5). Some pups were euthanized on PoN days 7 and 21; and samples were collected for quantification of trace element (n = 5), brain sialic acid concentration (n = 5), and Nissl staining using cresyl fast (n = 3). Results: The results of the trace elements quantification on PoN days 7 and 21 revealed significantly lower mean copper, zinc, and iron, and significantly higher calcium level in the group treated with 400 and 800 mg kg−1 of EATI when compared with the positive control group. EATI at a dose of 800 mg kg−1 showed improvement in memory and increased brain sialic acid. EATI 400 mg kg−1 increased only free sialic acid and had no effect on memory. Improvement in Nissl staining was observed in the EATI treated group on PoN day 7 and 21. Conclusion: The administration of EATI ameliorated AlCl3-induced neural toxicity.http://www.sciencedirect.com/science/article/pii/S2773050623000022PhytochemicalsNovel object recognitionTraced elementsBrain sialic acidNissl substanceAluminum toxicity |
| spellingShingle | Ibe Michael Usman, PhD Abel Nosereme Agbon, PhD Andrew Ekpeyong Ivang, PhD Akwu Bala Peter Adam Moyosore Afodun, PhD Akeem Ayodeji Okesina, PhD Victor Fischer, PhD Bot Yakubu Sunday, PhD Eric Osamu Aigbogun, Jr., PhD Ismail Onanuga, PhD Caroline Asekenye, MSc Ann Monima Lemuel, MSc Keneth Iceland Kasozi, PhD Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats Journal of Trace Elements and Minerals Phytochemicals Novel object recognition Traced elements Brain sialic acid Nissl substance Aluminum toxicity |
| title | Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats |
| title_full | Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats |
| title_fullStr | Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats |
| title_full_unstemmed | Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats |
| title_short | Ethyl acetate fraction of Tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal Wistar rats |
| title_sort | ethyl acetate fraction of tamarindus indica leaf ameliorates aluminium chloride induced neural damage in neonatal wistar rats |
| topic | Phytochemicals Novel object recognition Traced elements Brain sialic acid Nissl substance Aluminum toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2773050623000022 |
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