Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning.
Silk proteins are synthesized in the middle and posterior silk glands of silkworms, then transit into the anterior of the silk gland, where the silk fibers are produced, stored and processed. The mechanism of formation and spinning of the silk fibers has not been fully elucidated, and transcriptome...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4587926?pdf=render |
| _version_ | 1818996608261423104 |
|---|---|
| author | Huaipu Chang Tingcai Cheng Yuqian Wu Wenbo Hu Renwen Long Chun Liu Ping Zhao Qingyou Xia |
| author_facet | Huaipu Chang Tingcai Cheng Yuqian Wu Wenbo Hu Renwen Long Chun Liu Ping Zhao Qingyou Xia |
| author_sort | Huaipu Chang |
| collection | DOAJ |
| description | Silk proteins are synthesized in the middle and posterior silk glands of silkworms, then transit into the anterior of the silk gland, where the silk fibers are produced, stored and processed. The mechanism of formation and spinning of the silk fibers has not been fully elucidated, and transcriptome analyses specific to the anterior silk gland have not been reported. In the present study, we explored gene expression profiles in five regions of silk gland samples using the RNA-Seq method. As a result, there were 959,979,570 raw reads obtained, of which 583,068,172 reads were mapped to the silkworm genome. A total of 7419 genes were found to be expressed in terms of reads per kilobase of exon model per million mapped reads ≥ 5 in at least one sample. The gene numbers and expression levels of the expressed genes differed between these regions. The differentially expressed genes were analyzed, and 282 genes were detected as up-regulated in the anterior silk gland, compared with the other parts. Functions of these genes were addressed using the gene ontology and Kyoto Encyclopedia of Genes and Genomes databases, and seven key pathways were enriched. It suggested that the ion transportation, energy metabolism, protease inhibitors and cuticle proteins played essential roles in the process of silk formation and spinning in the anterior silk gland. In addition, 210 genes were found differently expressed between males and females, which should help to elucidate the mechanism of the quality difference in silk fibers from male and female silkworms. |
| first_indexed | 2024-12-20T21:32:26Z |
| format | Article |
| id | doaj.art-9b159a7675864e5f81fb9d46b63ae3bb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T21:32:26Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9b159a7675864e5f81fb9d46b63ae3bb2022-12-21T19:26:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013942410.1371/journal.pone.0139424Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning.Huaipu ChangTingcai ChengYuqian WuWenbo HuRenwen LongChun LiuPing ZhaoQingyou XiaSilk proteins are synthesized in the middle and posterior silk glands of silkworms, then transit into the anterior of the silk gland, where the silk fibers are produced, stored and processed. The mechanism of formation and spinning of the silk fibers has not been fully elucidated, and transcriptome analyses specific to the anterior silk gland have not been reported. In the present study, we explored gene expression profiles in five regions of silk gland samples using the RNA-Seq method. As a result, there were 959,979,570 raw reads obtained, of which 583,068,172 reads were mapped to the silkworm genome. A total of 7419 genes were found to be expressed in terms of reads per kilobase of exon model per million mapped reads ≥ 5 in at least one sample. The gene numbers and expression levels of the expressed genes differed between these regions. The differentially expressed genes were analyzed, and 282 genes were detected as up-regulated in the anterior silk gland, compared with the other parts. Functions of these genes were addressed using the gene ontology and Kyoto Encyclopedia of Genes and Genomes databases, and seven key pathways were enriched. It suggested that the ion transportation, energy metabolism, protease inhibitors and cuticle proteins played essential roles in the process of silk formation and spinning in the anterior silk gland. In addition, 210 genes were found differently expressed between males and females, which should help to elucidate the mechanism of the quality difference in silk fibers from male and female silkworms.http://europepmc.org/articles/PMC4587926?pdf=render |
| spellingShingle | Huaipu Chang Tingcai Cheng Yuqian Wu Wenbo Hu Renwen Long Chun Liu Ping Zhao Qingyou Xia Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. PLoS ONE |
| title | Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. |
| title_full | Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. |
| title_fullStr | Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. |
| title_full_unstemmed | Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. |
| title_short | Transcriptomic Analysis of the Anterior Silk Gland in the Domestic Silkworm (Bombyx mori) - Insight into the Mechanism of Silk Formation and Spinning. |
| title_sort | transcriptomic analysis of the anterior silk gland in the domestic silkworm bombyx mori insight into the mechanism of silk formation and spinning |
| url | http://europepmc.org/articles/PMC4587926?pdf=render |
| work_keys_str_mv | AT huaipuchang transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT tingcaicheng transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT yuqianwu transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT wenbohu transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT renwenlong transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT chunliu transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT pingzhao transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning AT qingyouxia transcriptomicanalysisoftheanteriorsilkglandinthedomesticsilkwormbombyxmoriinsightintothemechanismofsilkformationandspinning |