Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
Abstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, t...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-23887-1 |
| _version_ | 1818753944995758080 |
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| author | Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer |
| author_facet | Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer |
| author_sort | Ehsan Ghasemian |
| collection | DOAJ |
| description | Abstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, the frequency of Ct and CLOs was examined in the eyes of healthy Sudanese (control) participants and those with trachoma (case). We tested 96 children (54 cases and 42 controls) and 93 adults (51 cases and 42 controls) using broad-range Chlamydiae and Ct-specific (omcB) real-time PCR. Samples positive by broad-range Chlamydiae testing were subjected to DNA sequencing. Overall Chlamydiae prevalence was 36%. Sequences corresponded to unclassified and classified Chlamydiae. Ct infection rate was significantly higher in children (31.5%) compared to adults (0%) with trachoma (p < 0.0001). In general, 21.5% of adults and 4.2% of children tested positive for CLOs (p = 0.0003). Our findings are consistent with previous investigations describing the central role of Ct in trachoma among children. This is the first study examining human eyes for the presence of CLOs. We found an age-dependent distribution of CLO DNA in human eyes with significantly higher positivity in adults. Further studies are needed to understand the impact of CLOs in trachoma pathogenicity and/or protection. |
| first_indexed | 2024-12-18T05:15:25Z |
| format | Article |
| id | doaj.art-9b173ff4c2f144639dcfd7be29c0a66c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T05:15:25Z |
| publishDate | 2018-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9b173ff4c2f144639dcfd7be29c0a66c2022-12-21T21:19:47ZengNature PortfolioScientific Reports2045-23222018-05-018111610.1038/s41598-018-23887-1Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic regionEhsan Ghasemian0Aleksandra Inic-Kanada1Astrid Collingro2Florian Tagini3Elisabeth Stein4Hadeel Alchalabi5Nadine Schuerer6Darja Keše7Balgesa Elkheir Babiker8Nicole Borel9Gilbert Greub10Talin Barisani-Asenbauer11OCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaOCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaDepartment of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of ViennaInstitute of Microbiology, University of Lausanne and University HospitalOCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaOCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaOCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaInstitute of Microbiology and Immunology, Faculty of Medicine, University of LjubljanaFederal Ministry of HealthInstitute of Veterinary Pathology, Department of Pathobiology, Vetsuisse Faculty, University of ZurichInstitute of Microbiology, University of Lausanne and University HospitalOCUVAC – Center of Ocular Inflammation and Infection, Laura Bassi Centres of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of ViennaAbstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, the frequency of Ct and CLOs was examined in the eyes of healthy Sudanese (control) participants and those with trachoma (case). We tested 96 children (54 cases and 42 controls) and 93 adults (51 cases and 42 controls) using broad-range Chlamydiae and Ct-specific (omcB) real-time PCR. Samples positive by broad-range Chlamydiae testing were subjected to DNA sequencing. Overall Chlamydiae prevalence was 36%. Sequences corresponded to unclassified and classified Chlamydiae. Ct infection rate was significantly higher in children (31.5%) compared to adults (0%) with trachoma (p < 0.0001). In general, 21.5% of adults and 4.2% of children tested positive for CLOs (p = 0.0003). Our findings are consistent with previous investigations describing the central role of Ct in trachoma among children. This is the first study examining human eyes for the presence of CLOs. We found an age-dependent distribution of CLO DNA in human eyes with significantly higher positivity in adults. Further studies are needed to understand the impact of CLOs in trachoma pathogenicity and/or protection.https://doi.org/10.1038/s41598-018-23887-1 |
| spellingShingle | Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region Scientific Reports |
| title | Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region |
| title_full | Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region |
| title_fullStr | Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region |
| title_full_unstemmed | Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region |
| title_short | Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region |
| title_sort | detection of chlamydiaceae and chlamydia like organisms on the ocular surface of children and adults from a trachoma endemic region |
| url | https://doi.org/10.1038/s41598-018-23887-1 |
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