Sox2 Level Is a Determinant of Cellular Reprogramming Potential.
Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the rep...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3688988?pdf=render |
| _version_ | 1818847157557395456 |
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| author | Dong Wook Han Natalia Tapia Marcos J Araúzo-Bravo Kyung Tae Lim Kee Pyo Kim Kinarm Ko Hoon Taek Lee Hans R Schöler |
| author_facet | Dong Wook Han Natalia Tapia Marcos J Araúzo-Bravo Kyung Tae Lim Kee Pyo Kim Kinarm Ko Hoon Taek Lee Hans R Schöler |
| author_sort | Dong Wook Han |
| collection | DOAJ |
| description | Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate that the epiblast-derived pluripotent stem cells EpiSCs and P19 embryonal carcinoma cells (ECCs) exhibit a lower reprogramming potential than ESCs and F9 ECCs. In addition, we show that the low reprogramming ability is due to the lower levels of Sox2 in epiblast-derived stem cells. Consistent with this observation, overexpression of Sox2 enhances reprogramming efficiency. In summary, these findings suggest that a low reprogramming potential is a general feature of epiblast-derived stem cells and that the Sox2 level is a determinant of the cellular reprogramming potential. |
| first_indexed | 2024-12-19T05:56:59Z |
| format | Article |
| id | doaj.art-9b19138599fb498eb1d590d94f5e7fa5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T05:56:59Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9b19138599fb498eb1d590d94f5e7fa52022-12-21T20:33:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6759410.1371/journal.pone.0067594Sox2 Level Is a Determinant of Cellular Reprogramming Potential.Dong Wook HanNatalia TapiaMarcos J Araúzo-BravoKyung Tae LimKee Pyo KimKinarm KoHoon Taek LeeHans R SchölerEpiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate that the epiblast-derived pluripotent stem cells EpiSCs and P19 embryonal carcinoma cells (ECCs) exhibit a lower reprogramming potential than ESCs and F9 ECCs. In addition, we show that the low reprogramming ability is due to the lower levels of Sox2 in epiblast-derived stem cells. Consistent with this observation, overexpression of Sox2 enhances reprogramming efficiency. In summary, these findings suggest that a low reprogramming potential is a general feature of epiblast-derived stem cells and that the Sox2 level is a determinant of the cellular reprogramming potential.http://europepmc.org/articles/PMC3688988?pdf=render |
| spellingShingle | Dong Wook Han Natalia Tapia Marcos J Araúzo-Bravo Kyung Tae Lim Kee Pyo Kim Kinarm Ko Hoon Taek Lee Hans R Schöler Sox2 Level Is a Determinant of Cellular Reprogramming Potential. PLoS ONE |
| title | Sox2 Level Is a Determinant of Cellular Reprogramming Potential. |
| title_full | Sox2 Level Is a Determinant of Cellular Reprogramming Potential. |
| title_fullStr | Sox2 Level Is a Determinant of Cellular Reprogramming Potential. |
| title_full_unstemmed | Sox2 Level Is a Determinant of Cellular Reprogramming Potential. |
| title_short | Sox2 Level Is a Determinant of Cellular Reprogramming Potential. |
| title_sort | sox2 level is a determinant of cellular reprogramming potential |
| url | http://europepmc.org/articles/PMC3688988?pdf=render |
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