Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing
By directly converting solar energy and carbon dioxide into biobased products, cyanobacteria are promising chassis for photosynthetic biosynthesis. To make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales, exploring and engineering cyanobacterial chass...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-12-01
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| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X23000881 |
| _version_ | 1797372945453023232 |
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| author | Jinyu Cui Rongze Chen Huili Sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang Chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu |
| author_facet | Jinyu Cui Rongze Chen Huili Sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang Chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu |
| author_sort | Jinyu Cui |
| collection | DOAJ |
| description | By directly converting solar energy and carbon dioxide into biobased products, cyanobacteria are promising chassis for photosynthetic biosynthesis. To make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales, exploring and engineering cyanobacterial chassis and cell factories with fast growth rates and carbon fixation activities facing environmental stresses are of great significance. To simplify and accelerate the screening for fast-growing cyanobacteria strains, a method called Individual Cyanobacteria Vitality Tests and Screening (iCyanVS) was established. We show that the 13C incorporation ratio of carotenoids can be used to measure differences in cell growth and carbon fixation rates in individual cyanobacterial cells of distinct genotypes that differ in growth rates in bulk cultivations, thus greatly accelerating the process screening for fastest-growing cells. The feasibility of this approach is further demonstrated by phenotypically and then genotypically identifying individual cyanobacterial cells with higher salt tolerance from an artificial mutant library via Raman-activated gravity-driven encapsulation and sequencing. Therefore, this method should find broad applications in growth rate or carbon intake rate based screening of cyanobacteria and other photosynthetic cell factories. |
| first_indexed | 2024-03-08T18:43:09Z |
| format | Article |
| id | doaj.art-9b19b54273df49aba20a36a19eef07e1 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-03-08T18:43:09Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-9b19b54273df49aba20a36a19eef07e12023-12-29T04:45:39ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2023-12-0184708715Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencingJinyu Cui0Rongze Chen1Huili Sun2Yingyi Xue3Zhidian Diao4Jingyun Song5Xiaohang Wang6Jia Zhang7Chen Wang8Bo Ma9Jian Xu10Guodong Luan11Xuefeng Lu12Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, China; Dalian National Laboratory for Clean Energy, Dalian, 116023, China; Corresponding author. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Energy Institute, Qingdao, 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, China; Dalian National Laboratory for Clean Energy, Dalian, 116023, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.By directly converting solar energy and carbon dioxide into biobased products, cyanobacteria are promising chassis for photosynthetic biosynthesis. To make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales, exploring and engineering cyanobacterial chassis and cell factories with fast growth rates and carbon fixation activities facing environmental stresses are of great significance. To simplify and accelerate the screening for fast-growing cyanobacteria strains, a method called Individual Cyanobacteria Vitality Tests and Screening (iCyanVS) was established. We show that the 13C incorporation ratio of carotenoids can be used to measure differences in cell growth and carbon fixation rates in individual cyanobacterial cells of distinct genotypes that differ in growth rates in bulk cultivations, thus greatly accelerating the process screening for fastest-growing cells. The feasibility of this approach is further demonstrated by phenotypically and then genotypically identifying individual cyanobacterial cells with higher salt tolerance from an artificial mutant library via Raman-activated gravity-driven encapsulation and sequencing. Therefore, this method should find broad applications in growth rate or carbon intake rate based screening of cyanobacteria and other photosynthetic cell factories.http://www.sciencedirect.com/science/article/pii/S2405805X23000881CyanobacteriaSingle-cell Raman microspectroscopyStable isotope probingCarotenoidsIdentification |
| spellingShingle | Jinyu Cui Rongze Chen Huili Sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang Chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing Synthetic and Systems Biotechnology Cyanobacteria Single-cell Raman microspectroscopy Stable isotope probing Carotenoids Identification |
| title | Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing |
| title_full | Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing |
| title_fullStr | Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing |
| title_full_unstemmed | Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing |
| title_short | Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing |
| title_sort | culture free identification of fast growing cyanobacteria cells by raman activated gravity driven encapsulation and sequencing |
| topic | Cyanobacteria Single-cell Raman microspectroscopy Stable isotope probing Carotenoids Identification |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X23000881 |
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