Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage
Phytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from <i>Obesumbacterium proteus<...
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MDPI AG
2022-11-01
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| author | Maria A. Danilova Ekaterina Yu. Epova Elena V. Trubnikova Niyaz V. Badrutdinov Anastasya S. Kokoreva Maxim S. Pusev Yulia I. Deryabina Elena P. Isakova |
| author_facet | Maria A. Danilova Ekaterina Yu. Epova Elena V. Trubnikova Niyaz V. Badrutdinov Anastasya S. Kokoreva Maxim S. Pusev Yulia I. Deryabina Elena P. Isakova |
| author_sort | Maria A. Danilova |
| collection | DOAJ |
| description | Phytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from <i>Obesumbacterium proteus</i> (OPP) in yeast <i>Yarrowia lipolytica</i> as cytosolic protein (encapsulated OPP), where the capsule (yeast cell) protects the enzyme from unfavourable factors (acid medium and active proteolysis in stomachs) and releases it along with the substrate in the duodenum only. Here we report results of testing the encapsulated OPP on the model of a broiler chicken in comparison to a conventional phytase from <i>Aspergillus ficuum</i>. The encapsulated OPP at a dosage of 30 FYT/kg provided the maximum body weight of the chicken in the end of experiment equal or somewhat higher than in the control group, where the available phosphorus deficit was complemented with a mineral phosphorus supply. In contrast, the conventional soluble phytase at a dosage of 100 or 1000 FYT/kg was not able to compensate for the phosphorus deficit in the diet, although chemical analysis demonstrated much phosphorus in the diet in a non-accessible form. The encapsulated OPP decreased the residual P<sub>i</sub> in the chicken faeces by 2.1 times in comparison to the control when added to the diet, whereas the conventional phytases negligibly affected this parameter regardless of the dosage. |
| first_indexed | 2024-03-09T17:54:23Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:54:23Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9b1ca7d597b7427eb526781925b7d7672023-11-24T10:29:17ZengMDPI AGApplied Sciences2076-34172022-11-0112231199910.3390/app122311999Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low DosageMaria A. Danilova0Ekaterina Yu. Epova1Elena V. Trubnikova2Niyaz V. Badrutdinov3Anastasya S. Kokoreva4Maxim S. Pusev5Yulia I. Deryabina6Elena P. Isakova7Laboratory of Biotechnology and Genome Editing Methods, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, RussiaLaboratory of Chemical Physics of Bioanalytical Processes, Emanuel Institute of Biochemical Physics of RAS, Moscow 119334, RussiaLaboratory of Biotechnology and Genome Editing Methods, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, RussiaLLC Organic Park, Kazan 420095, RussiaLaboratory of Ecological and Evolutionary Biochemistry, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaLaboratory of Ecological and Evolutionary Biochemistry, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaLaboratory of Ecological and Evolutionary Biochemistry, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaLaboratory of Ecological and Evolutionary Biochemistry, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaPhytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from <i>Obesumbacterium proteus</i> (OPP) in yeast <i>Yarrowia lipolytica</i> as cytosolic protein (encapsulated OPP), where the capsule (yeast cell) protects the enzyme from unfavourable factors (acid medium and active proteolysis in stomachs) and releases it along with the substrate in the duodenum only. Here we report results of testing the encapsulated OPP on the model of a broiler chicken in comparison to a conventional phytase from <i>Aspergillus ficuum</i>. The encapsulated OPP at a dosage of 30 FYT/kg provided the maximum body weight of the chicken in the end of experiment equal or somewhat higher than in the control group, where the available phosphorus deficit was complemented with a mineral phosphorus supply. In contrast, the conventional soluble phytase at a dosage of 100 or 1000 FYT/kg was not able to compensate for the phosphorus deficit in the diet, although chemical analysis demonstrated much phosphorus in the diet in a non-accessible form. The encapsulated OPP decreased the residual P<sub>i</sub> in the chicken faeces by 2.1 times in comparison to the control when added to the diet, whereas the conventional phytases negligibly affected this parameter regardless of the dosage.https://www.mdpi.com/2076-3417/12/23/11999phytasemicroencapsulationbroilersanti-nutritional<i>Yarrowia lipolytica</i><i>Obesumbacterium proteus</i> |
| spellingShingle | Maria A. Danilova Ekaterina Yu. Epova Elena V. Trubnikova Niyaz V. Badrutdinov Anastasya S. Kokoreva Maxim S. Pusev Yulia I. Deryabina Elena P. Isakova Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage Applied Sciences phytase microencapsulation broilers anti-nutritional <i>Yarrowia lipolytica</i> <i>Obesumbacterium proteus</i> |
| title | Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage |
| title_full | Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage |
| title_fullStr | Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage |
| title_full_unstemmed | Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage |
| title_short | Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage |
| title_sort | encapsulated phytase produced by recombinant i yarrowia lipolytica i exhibits high efficiency on broiler chickens in low dosage |
| topic | phytase microencapsulation broilers anti-nutritional <i>Yarrowia lipolytica</i> <i>Obesumbacterium proteus</i> |
| url | https://www.mdpi.com/2076-3417/12/23/11999 |
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