Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase
In this study, magnetic nanoparticles Fe3O4synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AIDIC Servizi S.r.l.
2022-09-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | https://www.cetjournal.it/index.php/cet/article/view/12669 |
| _version_ | 1828112838052282368 |
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| author | Olga V. Grebennikova Aleksandrina M. Sulman Valentina G. Matveeva |
| author_facet | Olga V. Grebennikova Aleksandrina M. Sulman Valentina G. Matveeva |
| author_sort | Olga V. Grebennikova |
| collection | DOAJ |
| description | In this study, magnetic nanoparticles Fe3O4synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were obtained Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP. The activity of the synthesized biocatalysts was estimated spectrophotometrically in the oxidation reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The optimal conditions for this reaction were selected (pH 6.0, temperature 45 °C). It has been determined that biocatalysts Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP slightly decreased their activity during 5 consecutive experiments, by 19 and 15 %. |
| first_indexed | 2024-04-11T11:55:19Z |
| format | Article |
| id | doaj.art-9b20acb559f9428c92eec1d8c7a83d86 |
| institution | Directory Open Access Journal |
| issn | 2283-9216 |
| language | English |
| last_indexed | 2024-04-11T11:55:19Z |
| publishDate | 2022-09-01 |
| publisher | AIDIC Servizi S.r.l. |
| record_format | Article |
| series | Chemical Engineering Transactions |
| spelling | doaj.art-9b20acb559f9428c92eec1d8c7a83d862022-12-22T04:25:10ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162022-09-019410.3303/CET2294112Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root PeroxidaseOlga V. GrebennikovaAleksandrina M. SulmanValentina G. MatveevaIn this study, magnetic nanoparticles Fe3O4synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were obtained Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP. The activity of the synthesized biocatalysts was estimated spectrophotometrically in the oxidation reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The optimal conditions for this reaction were selected (pH 6.0, temperature 45 °C). It has been determined that biocatalysts Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP slightly decreased their activity during 5 consecutive experiments, by 19 and 15 %.https://www.cetjournal.it/index.php/cet/article/view/12669 |
| spellingShingle | Olga V. Grebennikova Aleksandrina M. Sulman Valentina G. Matveeva Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase Chemical Engineering Transactions |
| title | Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase |
| title_full | Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase |
| title_fullStr | Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase |
| title_full_unstemmed | Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase |
| title_short | Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase |
| title_sort | use of magnetic nanoparticles fe3o4 in the synthesis of biocatalysts based on horseradish root peroxidase |
| url | https://www.cetjournal.it/index.php/cet/article/view/12669 |
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